Abstract
Parental strain T lymphocyte injected into F1 mice respond to allogeneic MHC antigens and so induce the symptoms of a graft-versus-host reaction (GVHR). We have measured the local GVHR by the popliteal lymph node assay, and showed the suppression of the local GVHR in mice by treatment with the monoclonal antibody (MoAb) AMT-13 which is specific against the interleukin 2 (IL-2) receptor on activated mouse lymphocytes. The inhibitory effect of the AMT-13 administration was comparable with the suppression of the local GVHR by treatment with L3T4, an MoAb directed against the T helper subset. The L3T4 administration caused a dramatic decrease in the proportion of the cells with the L3T4 phenotype in the circulation and a marginal reduction of these cells in the lymph nodes. In contrast, the AMT-13 treated mice showed no changes in the distribution of the T lymphocyte subsets besides those in the GVHR-stimulated lymph nodes. Obviously, only the small subset of antigen-activated IL-2 receptor-bearing lymphocytes was influenced by treatment with AMT-13. MoAb directed against antigens whose expression is restricted to activated lymphocytes, such as the IL-2 receptor, might become useful for a short term immunosuppression with limited side effects.
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- Diamantstein T., Osawa H. Studies on the interleukin-2 receptor, its generation and dynamics using monoclonal anti-interleukin-2 receptor antibodies. Mol Immunol. 1984 Dec;21(12):1229–1236. doi: 10.1016/0161-5890(84)90015-4. [DOI] [PubMed] [Google Scholar]
- Gratama J. W., Jansen J., Lipovich R. A., Tanke H. J., Goldstein G., Zwaan F. E. Treatment of acute graft-versus-host disease with monoclonal antibody OKT3. Clinical results and effect on circulating T lymphocytes. Transplantation. 1984 Nov;38(5):469–474. doi: 10.1097/00007890-198411000-00005. [DOI] [PubMed] [Google Scholar]
- Jonker M., Goldstein G., Balner H. Effects of in vivo administration of monoclonal antibodies specific for human T cell subpopulations on the immune system in a rhesus monkey model. Transplantation. 1983 Jun;35(6):521–526. doi: 10.1097/00007890-198306000-00001. [DOI] [PubMed] [Google Scholar]
- Kupiec-Weglinski J. W., Diamantstein T., Tilney N. L., Strom T. B. Therapy with monoclonal antibody to interleukin 2 receptor spares suppressor T cells and prevents or reverses acute allograft rejection in rats. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2624–2627. doi: 10.1073/pnas.83.8.2624. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin P. J., Shulman H. M., Schubach W. H., Hansen J. A., Fefer A., Miller G., Thomas E. D. Fatal Epstein-Barr-virus-associated proliferation of donor B cells after treatment of acute graft-versus-host disease with a murine anti-T-cell antibody. Ann Intern Med. 1984 Sep;101(3):310–315. doi: 10.7326/0003-4819-101-3-310. [DOI] [PubMed] [Google Scholar]
- Michaelides M., Hogarth P. M., McKenzie I. F. The immunosuppressive effect of monoclonal anti-Lyt-1.1 antibodies in vivo. Eur J Immunol. 1981 Dec;11(12):1005–1012. doi: 10.1002/eji.1830111211. [DOI] [PubMed] [Google Scholar]
- Nakayama E., Uenaka A. Effect of in vivo administration of Lyt antibodies. Lyt phenotype of T cells in lymphoid tissues and blocking of tumor rejection. J Exp Med. 1985 Feb 1;161(2):345–355. doi: 10.1084/jem.161.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osawa H., Diamantstein T. A rat monoclonal antibody that binds specifically to mouse T lymphoblasts and inhibits IL 2 receptor functions: a putative anti-IL 2 receptor antibody. J Immunol. 1984 May;132(5):2445–2450. [PubMed] [Google Scholar]
- Remlinger K., Martin P. J., Hansen J. A., Doney K. C., Smith A., Deeg H. J., Sullivan K., Storb R., Thomas E. D. Murine monoclonal anti-T cell antibodies for treatment of steroid-resistant acute graft-versus-host disease. Hum Immunol. 1984 Jan;9(1):21–35. doi: 10.1016/0198-8859(84)90004-1. [DOI] [PubMed] [Google Scholar]