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. 1987 May;68(2):298–304.

HLA-DR antigen expression on T cells from cerebrospinal fluid in multiple sclerosis and aseptic meningo-encephalitis.

S Fredrikson 1, A Karlsson-Parra 1, T Olsson 1, H Link 1
PMCID: PMC1542708  PMID: 3308214

Abstract

HLA-DR expression on T lymphocytes in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and acute aseptic meningo-encephalitis (AM), and from blood only from healthy controls was examined by a new double-immunofluorescence labelling assay using species-specific second layers on prefixed cell samples. Thirteen of 16 patients with AM (81%) had an elevated percentage of DR positive T cells in CSF against only two of 20 patients with MS (10%). Our data indicate that AM, an acute infection of the central nervous system (CNS), is accompanied by accumulation in CSF of activated, DR positive T cells as a reflection of actively involved cellular immunity within the CNS, while this accumulation of DR positive T cells is not seen in MS, a chronic inflammatory CNS disease, despite some of the patients being examined during clinical exacerbations.

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Selected References

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  1. Alviggi L., Johnston C., Hoskins P. J., Tee D. E., Pyke D. A., Leslie R. D., Vergani D. Pathogenesis of insulin-dependent diabetes: a role for activated T lymphocytes. Lancet. 1984 Jul 7;2(8393):4–6. doi: 10.1016/s0140-6736(84)91994-9. [DOI] [PubMed] [Google Scholar]
  2. Bellamy A. S., Calder V. L., Feldmann M., Davison A. N. The distribution of interleukin-2 receptor bearing lymphocytes in multiple sclerosis: evidence for a key role of activated lymphocytes. Clin Exp Immunol. 1985 Aug;61(2):248–256. [PMC free article] [PubMed] [Google Scholar]
  3. Cotner T., Williams J. M., Christenson L., Shapiro H. M., Strom T. B., Strominger J. Simultaneous flow cytometric analysis of human T cell activation antigen expression and DNA content. J Exp Med. 1983 Feb 1;157(2):461–472. doi: 10.1084/jem.157.2.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evans R. L., Faldetta T. J., Humphreys R. E., Pratt D. M., Yunis E. J., Schlossman S. F. Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens. J Exp Med. 1978 Nov 1;148(5):1440–1445. doi: 10.1084/jem.148.5.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fu S. M., Chiorazzi N., Wang C. Y., Montrazeri G., Kunkel H. G., Ko H. S., Gottlieb A. B. Ia-bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity. J Exp Med. 1978 Nov 1;148(5):1423–1428. doi: 10.1084/jem.148.5.1423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Golaz J., Steck A., Moretta L. Activated T lymphocytes in patients with multiple sclerosis. Neurology. 1983 Oct;33(10):1371–1373. doi: 10.1212/wnl.33.10.1371. [DOI] [PubMed] [Google Scholar]
  7. Greaves M. F., Verbi W., Festenstein H., Papasteriadis C., Jaraquemada D., Hayward A. "Ia-like" antigens on human T cells. Eur J Immunol. 1979 May;9(5):356–362. doi: 10.1002/eji.1830090504. [DOI] [PubMed] [Google Scholar]
  8. Hafler D. A., Fox D. A., Manning M. E., Schlossman S. F., Reinherz E. L., Weiner H. L. In vivo activated T lymphocytes in the peripheral blood and cerebrospinal fluid of patients with multiple sclerosis. N Engl J Med. 1985 May 30;312(22):1405–1411. doi: 10.1056/NEJM198505303122201. [DOI] [PubMed] [Google Scholar]
  9. Hafler D. A., Hemler M. E., Christenson L., Williams J. M., Shapiro H. M., Strom T. B., Strominger J. L., Weiner H. L. Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases. Clin Immunol Immunopathol. 1985 Nov;37(2):163–171. doi: 10.1016/0090-1229(85)90147-3. [DOI] [PubMed] [Google Scholar]
  10. Haynes B. F., Hemler M. E., Mann D. L., Eisenbarth G. S., Shelhamer J., Mostowski H. S., Thomas C. A., Strominger J. L., Fauci A. S. Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. J Immunol. 1981 Apr;126(4):1409–1414. [PubMed] [Google Scholar]
  11. Hemler M. E., Sanchez-Madrid F., Flotte T. J., Krensky A. M., Burakoff S. J., Bhan A. K., Springer T. A., Strominger J. L. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J Immunol. 1984 Jun;132(6):3011–3018. [PubMed] [Google Scholar]
  12. Hercend T., Ritz J., Schlossman S. F., Reinherz E. L. Comparative expression of T9, T10, and Ia antigens on activated human T cell subsets. Hum Immunol. 1981 Nov;3(3):247–259. doi: 10.1016/0198-8859(81)90021-5. [DOI] [PubMed] [Google Scholar]
  13. Jackson R. A., Haynes B. F., Burch W. M., Shimizu K., Bowring M. A., Eisenbarth G. S. Ia+ T cells in new onset Graves' disease. J Clin Endocrinol Metab. 1984 Aug;59(2):187–190. doi: 10.1210/jcem-59-2-187. [DOI] [PubMed] [Google Scholar]
  14. Jackson R. A., Morris M. A., Haynes B. F., Eisenbarth G. S. Increased circulating Ia-antigen-bearing T cells in type I diabetes mellitus. N Engl J Med. 1982 Apr 1;306(13):785–788. doi: 10.1056/NEJM198204013061305. [DOI] [PubMed] [Google Scholar]
  15. Karlsson-Parra A., Forsum U., Klareskog L., Sjöberg O. A simple immunoenzyme batch staining method for the enumeration of peripheral human T lymphocyte subsets. J Immunol Methods. 1983 Nov 11;64(1-2):85–90. doi: 10.1016/0022-1759(83)90386-1. [DOI] [PubMed] [Google Scholar]
  16. Klareskog L., Trägårdh L., Lindblom J. B., Peterson P. A. Reactivity of a rabbit antiserum against highly purified HLA-DR antigens. Scand J Immunol. 1978 Mar;7(3):199–208. doi: 10.1111/j.1365-3083.1978.tb00444.x. [DOI] [PubMed] [Google Scholar]
  17. Ko H. S., Fu S. M., Winchester R. J., Yu D. T., Kunkel H. G. Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells. J Exp Med. 1979 Aug 1;150(2):246–255. doi: 10.1084/jem.150.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Link H., Kostulas V. Utility of isoelectric focusing of cerebrospinal fluid and serum on agarose evaluated for neurological patients. Clin Chem. 1983 May;29(5):810–815. [PubMed] [Google Scholar]
  19. Link H., Tibbling G. Principles of albumin and IgG analyses in neurological disorders. III. Evaluation of IgG synthesis within the central nervous system in multiple sclerosis. Scand J Clin Lab Invest. 1977 Sep;37(5):397–401. doi: 10.1080/00365517709091498. [DOI] [PubMed] [Google Scholar]
  20. Mittler R. S., Rao P. E., Talle M. A., Look R., Goldstein G. Cell membrane perturbation of resting T cells and thymocytes causes display of activation antigens. J Exp Med. 1983 Jul 1;158(1):99–111. doi: 10.1084/jem.158.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Noronha A. B., Richman D. P., Arnason B. G. Detection of in vivo stimulated cerebrospinal-fluid lymphocytes by flow cytometry in patients with multiple sclerosis. N Engl J Med. 1980 Sep 25;303(13):713–717. doi: 10.1056/NEJM198009253031301. [DOI] [PubMed] [Google Scholar]
  22. Noronha A., Richman D. P., Arnason B. G. Multiple sclerosis: activated cells in cerebrospinal fluid in acute exacerbations. Ann Neurol. 1985 Dec;18(6):722–725. doi: 10.1002/ana.410180617. [DOI] [PubMed] [Google Scholar]
  23. Olsson T., Kostulas V., Link H. Improved detection of oligoclonal IgG in cerebrospinal fluid by isoelectric focusing in agarose, double-antibody peroxidase labeling, and avidin-biotin amplification. Clin Chem. 1984 Jul;30(7):1246–1249. [PubMed] [Google Scholar]
  24. Pontesilli O., Chase H. P., Carotenuto P., Herberger M. J., Hayward A. R. T-lymphocyte subpopulations in insulin-dependent (type I) diabetes mellitus. Clin Exp Immunol. 1986 Jan;63(1):68–72. [PMC free article] [PubMed] [Google Scholar]
  25. Reinherz E. L., Parkman R., Rappeport J., Rosen F. S., Schlossman S. F. Aberrations of suppressor T cells in human graft-versus-host disease. N Engl J Med. 1979 May 10;300(19):1061–1068. doi: 10.1056/NEJM197905103001901. [DOI] [PubMed] [Google Scholar]
  26. SCHUMACHER G. A., BEEBE G., KIBLER R. F., KURLAND L. T., KURTZKE J. F., MCDOWELL F., NAGLER B., SIBLEY W. A., TOURTELLOTTE W. W., WILLMON T. L. PROBLEMS OF EXPERIMENTAL TRIALS OF THERAPY IN MULTIPLE SCLEROSIS: REPORT BY THE PANEL ON THE EVALUATION OF EXPERIMENTAL TRIALS OF THERAPY IN MULTIPLE SCLEROSIS. Ann N Y Acad Sci. 1965 Mar 31;122:552–568. doi: 10.1111/j.1749-6632.1965.tb20235.x. [DOI] [PubMed] [Google Scholar]
  27. Tibbling G., Link H., Ohman S. Principles of albumin and IgG analyses in neurological disorders. I. Establishment of reference values. Scand J Clin Lab Invest. 1977 Sep;37(5):385–390. doi: 10.1080/00365517709091496. [DOI] [PubMed] [Google Scholar]
  28. Traugott U., Reinherz E. L., Raine C. S. Multiple sclerosis: distribution of T cell subsets within active chronic lesions. Science. 1983 Jan 21;219(4582):308–310. doi: 10.1126/science.6217550. [DOI] [PubMed] [Google Scholar]
  29. Tsudo M., Uchiyama T., Uchino H. Expression of Tac antigen on activated normal human B cells. J Exp Med. 1984 Aug 1;160(2):612–617. doi: 10.1084/jem.160.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Winchester R. J., Kunkel H. G. The human Ia system. Adv Immunol. 1979;28:221–292. [PubMed] [Google Scholar]
  31. Yu D. T., Winchester R. J., Fu S. M., Gibofsky A., Ko H. S., Kunkel H. G. Peripheral blood Ia-positive T cells. Increases in certain diseases and after immunization. J Exp Med. 1980 Jan 1;151(1):91–100. doi: 10.1084/jem.151.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]

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