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. 1987 Jun;68(3):648–654.

Cerebrospinal fluid interleukin 1 like activity during chronic relapsing experimental allergic encephalomyelitis.

J A Symons 1, R V Bundick 1, A J Suckling 1, M G Rumsby 1
PMCID: PMC1542740  PMID: 3498582

Abstract

Cerebrospinal fluid (CSF) and plasma samples were taken from strain 13 guinea pigs in various stages of chronic relapsing experimental allergic encephalomyelitis (CREAE). The samples were assayed for interleukin 1 (IL-1) in the C3H/Hej mouse thymocyte assay. After removal of inhibitors, IL-1 was detectable in low amounts in plasma (5 U/ml) throughout the course of the disease but was raised in the acute phase (12 U/ml). CSF IL-1 was, however, only present in low amounts (6 U/ml) during the acute phase but was elevated (18 U/ml) during the chronic stages of CREAE. During the relapse phase levels of IL-1 correlated with the total leucocyte count in the CSF. On gel filtration of CSF, IL-1 activity eluted at approximately 15 kD and could not be attributed to leakage of plasma IL-1 during CSF puncture or IL-2 activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bevilacqua M. P., Pober J. S., Wheeler M. E., Cotran R. S., Gimbrone M. A., Jr Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines. J Clin Invest. 1985 Nov;76(5):2003–2011. doi: 10.1172/JCI112200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De Vos C., Libert W. A simple rapid method for detection of IL-2 in a physiological medium. J Immunol Methods. 1984 Nov 30;74(2):375–384. doi: 10.1016/0022-1759(84)90306-5. [DOI] [PubMed] [Google Scholar]
  3. Degré M., Dahl H., Vandvik B. Interferon in the serum and cerebrospinal fluid in patients with multiple sclerosis and other neurological disorders. Acta Neurol Scand. 1976 Feb;53(2):152–160. doi: 10.1111/j.1600-0404.1976.tb04333.x. [DOI] [PubMed] [Google Scholar]
  4. Dinarello C. A. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51–95. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
  5. Estes J. E., Pledger W. J., Gillespie G. Y. Macrophage-derived growth factor for fibroblasts and Interleukin-1 are distinct entities. J Leukoc Biol. 1984 Jan;35(1):115–129. doi: 10.1002/jlb.35.1.115. [DOI] [PubMed] [Google Scholar]
  6. Farrar J. J., Fuller-Farrar J., Simon P. L., Hilfiker M. L., Stadler B. M., Farrar W. L. Thymoma production of T cell growth factor (Interleukin 2). J Immunol. 1980 Dec;125(6):2555–2558. [PubMed] [Google Scholar]
  7. Fontana A., Kristensen F., Dubs R., Gemsa D., Weber E. Production of prostaglandin E and an interleukin-1 like factor by cultured astrocytes and C6 glioma cells. J Immunol. 1982 Dec;129(6):2413–2419. [PubMed] [Google Scholar]
  8. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  9. Giulian D., Baker T. J., Shih L. C., Lachman L. B. Interleukin 1 of the central nervous system is produced by ameboid microglia. J Exp Med. 1986 Aug 1;164(2):594–604. doi: 10.1084/jem.164.2.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Giulian D., Lachman L. B. Interleukin-1 stimulation of astroglial proliferation after brain injury. Science. 1985 Apr 26;228(4698):497–499. doi: 10.1126/science.3872478. [DOI] [PubMed] [Google Scholar]
  11. Gorczynski R. M., Keystone E. J. Interleukin-1-like activity in human cerebrospinal fluid. Immunol Lett. 1986 Oct 15;13(5):231–235. doi: 10.1016/0165-2478(86)90107-0. [DOI] [PubMed] [Google Scholar]
  12. Keith A. B., McDermott J. R. Optimum conditions for inducing chronic relapsing experimental allergic encephalomyelitis in guinea pigs. J Neurol Sci. 1980 Jun;46(3):353–364. doi: 10.1016/0022-510x(80)90060-x. [DOI] [PubMed] [Google Scholar]
  13. Kirby J. A., Suckling A. J., Rumsby M. G. Chronic relapsing experimental allergic encephalomyelitis. The presence in the cerebrospinal fluid of factors chemotactic for monocytes. J Neuroimmunol. 1983 Dec;5(3):271–281. doi: 10.1016/0165-5728(83)90047-4. [DOI] [PubMed] [Google Scholar]
  14. Miossec P., Dinarello C. A., Ziff M. Interleukin-1 lymphocyte chemotactic activity in rheumatoid arthritis synovial fluid. Arthritis Rheum. 1986 Apr;29(4):461–470. doi: 10.1002/art.1780290402. [DOI] [PubMed] [Google Scholar]
  15. Miossec P., Yu C. L., Ziff M. Lymphocyte chemotactic activity of human interleukin 1. J Immunol. 1984 Oct;133(4):2007–2011. [PubMed] [Google Scholar]
  16. Postlethwaite A. E., Kang A. H. Characterization of fibroblast proliferation factors elaborated by antigen- and mitogen-stimulated guinea pig lymph node cells: differentiation from lymphocyte-derived chemotactic factor for fibroblasts, lymphocyte mitogenic factor, and interleukin 1. Cell Immunol. 1982 Oct;73(1):169–178. doi: 10.1016/0008-8749(82)90445-2. [DOI] [PubMed] [Google Scholar]
  17. Reiber H., Schunck O. Suboccipital puncture of guinea pigs. Lab Anim. 1983 Jan;17(1):25–27. doi: 10.1258/002367783781070876. [DOI] [PubMed] [Google Scholar]
  18. Suckling A. J., Wilson N. R., Kirby J. A., Rumsby M. G. Chronic relapsing experimental allergic encephalomyelitis: cerebrospinal fluid cytology and a comparison with meningeal and spinal cord pathology. Neuropathol Appl Neurobiol. 1983 May-Jun;9(3):237–249. doi: 10.1111/j.1365-2990.1983.tb00111.x. [DOI] [PubMed] [Google Scholar]
  19. Symons J. A., Suckling A. J., Bundick R. V., Rumsby M. G. Chronic relapsing experimental allergic encephalomyelitis. Inhibition of lymphocyte mitogenesis by disease-related serum factors. J Neuroimmunol. 1986 Aug;12(2):163–169. doi: 10.1016/0165-5728(86)90029-9. [DOI] [PubMed] [Google Scholar]
  20. Wiśniewski H. M., Keith A. B. Chronic relapsing experimental allergic encephalomyelitis: an experimental model of multiple sclerosis. Ann Neurol. 1977 Feb;1(2):144–148. doi: 10.1002/ana.410010207. [DOI] [PubMed] [Google Scholar]
  21. Woodroofe M. N., Bellamy A. S., Feldmann M., Davison A. N., Cuzner M. L. Immunocytochemical characterisation of the immune reaction in the central nervous system in multiple sclerosis. Possible role for microglia in lesion growth. J Neurol Sci. 1986 Jul;74(2-3):135–152. doi: 10.1016/0022-510x(86)90100-0. [DOI] [PubMed] [Google Scholar]

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