Skip to main content
PMC home page
British Medical Journal (Clinical Research Ed.) logoLink to British Medical Journal (Clinical Research Ed.)
. 1983 Sep 17;287(6395):784–788. doi: 10.1136/bmj.287.6395.784

Presence of human papillomavirus DNA sequences in cervical intraepithelial neoplasia.

D J McCance, P G Walker, J L Dyson, D V Coleman, A Singer
PMCID: PMC1549159  PMID: 6311316

Abstract

Twenty two patients referred to a district colposcopy clinic because of an abnormal cervical cytology report or a suspicious cervix and found to have a cervical epithelial abnormality were studied. The techniques of cytology, histology, immunohistochemistry, and DNA-DNA hybridisation were used to detect infection by human papillomavirus. Using an indirect immunoalkaline phosphatase technique human papillomavirus antigen was found in biopsy specimens from six of the 22 patients and DNA of papillomavirus type 6 in biopsy specimens from 13 of these women, including four out of six whose histological diagnosis was cervical intraepithelial neoplasia grade 3. In eight cases where cytological, colposcopical, and histological investigations all indicated the presence of wart virus infection, papillomavirus type 6 DNA was found in seven. Papillomavirus type 6 DNA was found in more than half of the proved cases of cervical intraepithelial neoplasia. The presence of this viral DNA in women with no cervical abnormality is to be studied.

Full text

PDF
784

Images in this article

786FIG 1
on p.786
786FIG 2
on p.786
786FIG 3
on p.786
787FIG 4 (a)
on p.787
787FIG 4 (b)
on p.787

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buckley C. H., Butler E. B., Fox H. Cervical intraepithelial neoplasia. J Clin Pathol. 1982 Jan;35(1):1–13. doi: 10.1136/jcp.35.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chesters P. M., Heritage J., McCance D. J. Persistence of DNA sequences of BK virus and JC virus in normal human tissues and in diseased tissues. J Infect Dis. 1983 Apr;147(4):676–684. doi: 10.1093/infdis/147.4.676. [DOI] [PubMed] [Google Scholar]
  3. Ferenczy A., Braun L., Shah K. V. Human papillomavirus (HPV) in condylomatous lesions of cervix. Am J Surg Pathol. 1981 Oct;5(7):661–670. doi: 10.1097/00000478-198110000-00008. [DOI] [PubMed] [Google Scholar]
  4. Gissmann L., Diehl V., Schultz-Coulon H. J., zur Hausen H. Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma. J Virol. 1982 Oct;44(1):393–400. doi: 10.1128/jvi.44.1.393-400.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gissmann L., Wolnik L., Ikenberg H., Koldovsky U., Schnürch H. G., zur Hausen H. Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proc Natl Acad Sci U S A. 1983 Jan;80(2):560–563. doi: 10.1073/pnas.80.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gissmann L., deVilliers E. M., zur Hausen H. Analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 DNA. Int J Cancer. 1982 Feb 15;29(2):143–146. doi: 10.1002/ijc.2910290205. [DOI] [PubMed] [Google Scholar]
  7. Green M., Brackmann K. H., Sanders P. R., Loewenstein P. M., Freel J. H., Eisinger M., Switlyk S. A. Isolation of a human papillomavirus from a patient with epidermodysplasia verruciformis: presence of related viral DNA genomes in human urogenital tumors. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4437–4441. doi: 10.1073/pnas.79.14.4437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heritage J., Chesters P. M., McCance D. J. The persistence of papovavirus BK DNA sequences in normal human renal tissue. J Med Virol. 1981;8(2):143–150. doi: 10.1002/jmv.1890080208. [DOI] [PubMed] [Google Scholar]
  9. Jarrett W. F., McNeil P. E., Grimshaw W. T., Selman I. E., McIntyre W. I. High incidence area of cattle cancer with a possible interaction between an environmental carcinogen and a papilloma virus. Nature. 1978 Jul 20;274(5668):215–217. doi: 10.1038/274215a0. [DOI] [PubMed] [Google Scholar]
  10. Jenson A. B., Rosenthal J. D., Olson C., Pass F., Lancaster W. D., Shah K. Immunologic relatedness of papillomaviruses from different species. J Natl Cancer Inst. 1980 Mar;64(3):495–500. [PubMed] [Google Scholar]
  11. KLEINSASSER O. Uber die gut- und bösartigen Formen der Kehlkopfpapillome und deren histologisches und klinisches Bild. Arch Ohren Nasen Kehlkopfheilkd. 1958;174(1):44–69. [PubMed] [Google Scholar]
  12. Kessler I. I. Human cervical cancer as a venereal disease. Cancer Res. 1976 Feb;36(2 Pt 2):783–791. [PubMed] [Google Scholar]
  13. Kurman R. J., Shah K. H., Lancaster W. D., Jenson A. B. Immunoperoxidase localization of papillomavirus antigens in cervical dysplasia and vulvar condylomas. Am J Obstet Gynecol. 1981 Aug 15;140(8):931–935. doi: 10.1016/0002-9378(81)90087-9. [DOI] [PubMed] [Google Scholar]
  14. Law M. F., Lowy D. R., Dvoretzky I., Howley P. M. Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc Natl Acad Sci U S A. 1981 May;78(5):2727–2731. doi: 10.1073/pnas.78.5.2727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MARSH M. R. Papilloma of the cervix. Am J Obstet Gynecol. 1952 Aug;64(2):281–291. doi: 10.1016/0002-9378(52)90298-6. [DOI] [PubMed] [Google Scholar]
  16. Meisels A., Fortin R. Condylomatous lesions of the cervix and vagina. I. Cytologic patterns. Acta Cytol. 1976 Nov-Dec;20(6):505–509. [PubMed] [Google Scholar]
  17. Meisels A., Fortin R., Roy M. Condylomatous lesions of the cervix. II. Cytologic, colposcopic and histopathologic study. Acta Cytol. 1977 May-Jun;21(3):379–390. [PubMed] [Google Scholar]
  18. Moar M. H., Campo M. S., Laird H., Jarrett W. F. Persistence of non-integrated viral DNA in bovine cells transformed in vitro by bovine papillomavirus type 2. Nature. 1981 Oct 29;293(5835):749–751. doi: 10.1038/293749a0. [DOI] [PubMed] [Google Scholar]
  19. Mounts P., Shah K. V., Kashima H. Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5425–5429. doi: 10.1073/pnas.79.17.5425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oriel J. D. Natural history of genital warts. Br J Vener Dis. 1971 Feb;47(1):1–13. doi: 10.1136/sti.47.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Orth G., Jablonska S., Favre M., Croissant O., Jarzabek-Chorzelska M., Rzesa G. Characterization of two types of human papillomaviruses in lesions of epidermodysplasia verruciformis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1537–1541. doi: 10.1073/pnas.75.3.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ostrow R. S., Bender M., Niimura M., Seki T., Kawashima M., Pass F., Faras A. J. Human papillomavirus DNA in cutaneous primary and metastasized squamous cell carcinomas from patients with epidermodysplasia verruciformis. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1634–1638. doi: 10.1073/pnas.79.5.1634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Purola E., Savia E. Cytology of gynecologic condyloma acuminatum. Acta Cytol. 1977 Jan-Feb;21(1):26–31. [PubMed] [Google Scholar]
  24. RAFTERY A., PAYNE W. S. Condyloma acuminata of the cervix. Obstet Gynecol. 1954 Nov;4(5):581–584. [PubMed] [Google Scholar]
  25. Reid R., Laverty C. R., Coppleson M., Isarangkul W., Hills E. Noncondylomatous cervical wart virus infection. Obstet Gynecol. 1980 Apr;55(4):476–483. [PubMed] [Google Scholar]
  26. Reid R., Stanhope C. R., Herschman B. R., Booth E., Phibbs G. D., Smith J. P. Genital warts and cervical cancer. I. Evidence of an association between subclinical papillomavirus infection and cervical malignancy. Cancer. 1982 Jul 15;50(2):377–387. doi: 10.1002/1097-0142(19820715)50:2<377::aid-cncr2820500236>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  27. Rotkin I. D. A comparison review of key epidemiological studies in cervical cancer related to current searches for transmissible agents. Cancer Res. 1973 Jun;33(6):1353–1367. [PubMed] [Google Scholar]
  28. Shafeek M. A., Osman M. I., Hussein M. A. Carcinoma of the vulva arising in condylomata acuminata. Obstet Gynecol. 1979 Jul;54(1):120–123. doi: 10.1097/00006250-197907000-00027. [DOI] [PubMed] [Google Scholar]
  29. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  30. Ulbright T. M., Stehman F. B., Roth L. M., Ehrlich C. E., Ransburg R. C. Bowenoid dysplasia of the vulva. Cancer. 1982 Dec 15;50(12):2910–2919. doi: 10.1002/1097-0142(19821215)50:12<2910::aid-cncr2820501234>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
  31. Woodruff J. D., Braun L., Cavalieri R., Gupta P., Pass F., Shah K. V. Immunologic identification of papillomavirus antigen in condyloma tissues from the female genital tract. Obstet Gynecol. 1980 Dec;56(6):727–732. [PubMed] [Google Scholar]
  32. zur Hausen H. Human genital cancer: synergism between two virus infections or synergism between a virus infection and initiating events? Lancet. 1982 Dec 18;2(8312):1370–1372. doi: 10.1016/s0140-6736(82)91273-9. [DOI] [PubMed] [Google Scholar]

Articles from British Medical Journal (Clinical research ed.) are provided here courtesy of BMJ Publishing Group

RESOURCES