Skip to main content
NCBI home page
Gut logoLink to Gut
. 1969 Aug;10(8):685–689. doi: 10.1136/gut.10.8.685

An isotope technique for measuring lactose absorption

P R Salmon, A E Read, C F McCarthy
PMCID: PMC1552918  PMID: 5810982

Abstract

Expired radiocarbon dioxide has been collected by a simple autotitration method following the ingestion of lactose-1-14C. With this method, which is suitable for clinical use, 12 subjects with alactasia have been readily separated from 24 normals, both groups being defined by strict criteria.

This test, which may be used to measure the absorption of other sugars, is especially suitable for population surveys and may be used to investigate the distribution of disaccharidase deficiency.

A further advantage is that false low readings resulting from rapid plasma clearance of absorbed sugar do not occur with this method although they may do so in up to one in three lactose tolerance tests, thereby overestimating the prevalence of alactasia.

Full text

PDF
685

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGESS E. A., LEVIN B., MAHALANABIS D., TONGE R. E. HEREDITARY SUCROSE INTOLERANCE: LEVELS OF SUCRASE ACTIVITY IN JEJUNAL MUCOSA. Arch Dis Child. 1964 Oct;39:431–443. doi: 10.1136/adc.39.207.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COZZETTO F. J. RADIOCARBON ESTIMATES OF INTESTINAL ABSORPTION. STUDIES OF BREATH EXCRETION OF C14O2 AFTER INGESTION OF LABELED FATTY ACIDS, GLUCOSE, AND LACTOSE. Am J Dis Child. 1964 Jun;107:605–611. [PubMed] [Google Scholar]
  3. GODFREY P., SNYDER F. A procedure for in vivo C-14-O2 collection and subsequent scintillation counting. Anal Biochem. 1962 Oct;4:310–315. doi: 10.1016/0003-2697(62)90092-1. [DOI] [PubMed] [Google Scholar]
  4. KINNEY J. M., MORGAN A. P., DOMINGUES F. J., GILDNER K. J. A METHOD FOR CONTINUOUS MEASUREMENT OF GAS EXCHANGE AND EXPIRED RADIOACTIVITY IN ACUTELY ILL PATIENTS. Metabolism. 1964 Mar;13:205–211. doi: 10.1016/0026-0495(64)90099-x. [DOI] [PubMed] [Google Scholar]
  5. Kaihara S., Wagner H. N., Jr Measurement of intestinal fat absorption with carbon-14 labeled tracers. J Lab Clin Med. 1968 Mar;71(3):400–411. [PubMed] [Google Scholar]
  6. LEROY G. V., OKITA G. T., TOCUS E. C., CHARLESTON D. Continuous measurement of specific activity of C14-labeled carbon dioxide in expired air. Int J Appl Radiat Isot. 1960 Feb;7:273–286. doi: 10.1016/0020-708x(60)90116-2. [DOI] [PubMed] [Google Scholar]
  7. MARKS V. An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels. Clin Chim Acta. 1959 May;4(3):395–400. doi: 10.1016/0009-8981(59)90110-x. [DOI] [PubMed] [Google Scholar]
  8. McGill D. B., Newcomer A. D. Comparison of venous and capillary blood samples in lactose tolerance testing. Gastroenterology. 1967 Sep;53(3):371–374. [PubMed] [Google Scholar]
  9. Newcomer A. D., McGill D. B. Lactose tolerance tests in adults with normal lactase activity. Gastroenterology. 1966 Mar;50(3):340–346. [PubMed] [Google Scholar]
  10. SCHWABE A. D., COZZETTO F. J., BENNETTLR, MELLINKOFF S. M. Estimation of fat absorption by monitoring of expired radio-active carbon dioxide after feeding a radioactive fat. Gastroenterology. 1962 Mar;42:285–291. [PubMed] [Google Scholar]
  11. Sammons H. G., Morgan D. B., Frazer A. C., Montgomery R. D., Philip W. M., Phillips M. J. Modification in the xylose absorption test as an index of intestinal function. Gut. 1967 Aug;8(4):348–353. doi: 10.1136/gut.8.4.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TOLBERT B. M., KIRK M., BAKER E. M. Continuous C14O2 and CO2 excretion studies in experimental animals. Am J Physiol. 1956 May;185(2):269–274. doi: 10.1152/ajplegacy.1956.185.2.269. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES