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. 1995 Oct;102(1):137–143. doi: 10.1111/j.1365-2249.1995.tb06647.x

Rabbit polymorphonuclear granulocyte function during ethanol administration--migration and oxidative responses in a joint with immune complex synovitis.

E Nilsson 1, P Thomsen 1, L Ericson 1, J Palmblad 1
PMCID: PMC1553331  PMID: 7554380

Abstract

Functional impairments of polymorphonuclear granulocytes (PMN) are believed to contribute to hampered inflammation and host defence in alcoholics. We studied effects of i.v. ethanol administration on PMN responses in rabbits during induction of a knee-joint synovitis. The synovitis conferred systemic effects, since chemiluminescent responses of peripheral blood PMN to opsonized zymosan and phorpbol myristate acetate (PMA) increased 6.4- and 17.9-fold, respectively. Chemiluminescent responses of synovial PMN were further amplified. This up-regulation was reduced to 33% in rabbits treated with ethanol when opsonized zymosan was used as the PMN stimulus; in contrast, PMA responses were unaffected. The appearance and migration of PMN to the synovitis joint were normal despite a blood ethanol concentration of 0.5%. Thus, ethanol impaired release of oxygen metabolites from PMN, but not the delivery of cells at an inflammatory site.

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Selected References

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  1. Adams H. G., Jordan C. Infections in the alcoholic. Med Clin North Am. 1984 Jan;68(1):179–200. doi: 10.1016/s0025-7125(16)31249-4. [DOI] [PubMed] [Google Scholar]
  2. Arm J. P., Walport M. J., Lee T. H. Expression of complement receptors type 1 (CR1) and type 3 (CR3) on circulating granulocytes in experimentally provoked asthma. J Allergy Clin Immunol. 1989 Mar;83(3):649–655. doi: 10.1016/0091-6749(89)90078-x. [DOI] [PubMed] [Google Scholar]
  3. Barbour A. G., Allred C. D., Solberg C. O., Hill H. R. Chemiluminescence by polymorphonuclear leukocytes from patients with active bacterial infection. J Infect Dis. 1980 Jan;141(1):14–26. doi: 10.1093/infdis/141.1.14. [DOI] [PubMed] [Google Scholar]
  4. Bass D. A., Olbrantz P., Szejda P., Seeds M. C., McCall C. E. Subpopulations of neutrophils with increased oxidative product formation in blood of patients with infection. J Immunol. 1986 Feb 1;136(3):860–866. [PubMed] [Google Scholar]
  5. Biemond P., Swaak A. J., Penders J. M., Beindorff C. M., Koster J. F. Superoxide production by polymorphonuclear leucocytes in rheumatoid arthritis and osteoarthritis: in vivo inhibition by the antirheumatic drug piroxicam due to interference with the activation of the NADPH-oxidase. Ann Rheum Dis. 1986 Mar;45(3):249–255. doi: 10.1136/ard.45.3.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bjursten L. M., Bagge U., Ahlstedt S. Joint inflammation in rabbits induced by preformed immune complexes. Int Arch Allergy Appl Immunol. 1981;64(1):67–71. doi: 10.1159/000232675. [DOI] [PubMed] [Google Scholar]
  7. Bjursten L. M., Thomsen P., Ahlstedt S., Bagge U. The kinetics of leucocyte migration into rabbit knee joints elicited by preformed immune complexes with different in vitro characteristics. Immunology. 1983 Jun;49(2):205–213. [PMC free article] [PubMed] [Google Scholar]
  8. Brayton R. G., Stokes P. E., Schwartz M. S., Louria D. B. Effect of alcohol and various diseases on leukocyte mobilization, phagocytosis and intracellular bacterial killing. N Engl J Med. 1970 Jan 15;282(3):123–128. doi: 10.1056/NEJM197001152820303. [DOI] [PubMed] [Google Scholar]
  9. Briheim G., Coble B., Stendahl O., Dahlgren C. Exudate polymorphonuclear leukocytes isolated from skin chambers are primed for enhanced response to subsequent stimulation with chemoattractant f-Met-Leu-Phe and C3-opsonized yeast particles. Inflammation. 1988 Apr;12(2):141–152. doi: 10.1007/BF00916397. [DOI] [PubMed] [Google Scholar]
  10. Buckley R. M., Ventura E. S., MacGregor R. R. Propranolol antagonizes the anti-inflammatory effect of alcohol and improves survival of infected intoxicated rabbits. J Clin Invest. 1978 Sep;62(3):554–559. doi: 10.1172/JCI109160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Follin P., Briheim G., Sandstedt S., Dahlgren C. Human neutrophil chemiluminescence and f-Meth-Leu-Phe receptor exposure in bacterial infections. APMIS. 1989 Jul;97(7):585–590. doi: 10.1111/j.1699-0463.1989.tb00447.x. [DOI] [PubMed] [Google Scholar]
  12. Gluckman S. J., MacGregor R. R. Effect of acute alcohol intoxication on granulocyte mobilization and kinetics. Blood. 1978 Sep;52(3):551–559. [PubMed] [Google Scholar]
  13. Greizerstein H. B. Indomethacin-ethanol interactions on acute inflammation. Subst Alcohol Actions Misuse. 1983;4(6):393–399. [PubMed] [Google Scholar]
  14. Hallengren B., Forsgren A. Effect of alcohol on chemotaxis, adherence and phagocytosis of human polymorphonuclear leucocytes. Acta Med Scand. 1978;204(1-2):43–48. doi: 10.1111/j.0954-6820.1978.tb08396.x. [DOI] [PubMed] [Google Scholar]
  15. Hed J., Berg O., Forslid J., Halldén G., Lärka-Rafner G. The expression of CR1 and CR3 on non-modulated and modulated granulocytes of healthy blood donors as measured by flow cytofluorometry. Scand J Immunol. 1988 Sep;28(3):339–344. doi: 10.1111/j.1365-3083.1988.tb01458.x. [DOI] [PubMed] [Google Scholar]
  16. Leibowitz H. M., Ryan W., Kupferman A., Vitale J. J. The effect of alcohol intoxication on inflammation of the cornea. Arch Ophthalmol. 1985 May;103(5):723–725. doi: 10.1001/archopht.1985.01050050115029. [DOI] [PubMed] [Google Scholar]
  17. MacGregor R. R., Safford M., Shalit M. Effect of ethanol on functions required for the delivery of neutrophils to sites of inflammation. J Infect Dis. 1988 Apr;157(4):682–689. doi: 10.1093/infdis/157.4.682. [DOI] [PubMed] [Google Scholar]
  18. MacGregor R. R., Spagnuolo P. J., Lentnek A. L. Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system. N Engl J Med. 1974 Sep 26;291(13):642–646. doi: 10.1056/NEJM197409262911302. [DOI] [PubMed] [Google Scholar]
  19. MacGregor R. R. The effect of anti-inflammatory agents and inflammation on granulocyte adherence. Evidence for regulation by plasma factors. Am J Med. 1976 Nov;61(5):597–607. doi: 10.1016/0002-9343(76)90137-6. [DOI] [PubMed] [Google Scholar]
  20. Nilsson E., Andersson T., Fällman M., Rosendahl K., Palmblad J. Effects of ethanol on the chemotactic peptide-induced second messenger generation and superoxide production in polymorphonuclear leukocytes. J Infect Dis. 1992 Oct;166(4):854–860. doi: 10.1093/infdis/166.4.854. [DOI] [PubMed] [Google Scholar]
  21. Nilsson E., Lindström P., Patarroyo M., Ringertz B., Lerner R., Rincon J., Palmblad J. Ethanol impairs certain aspects of neutrophil adhesion in vitro: comparisons with inhibition of expression of the CD18 antigen. J Infect Dis. 1991 Mar;163(3):591–597. doi: 10.1093/infdis/163.3.591. [DOI] [PubMed] [Google Scholar]
  22. Nilsson E., Palmblad J. Effects of ethanol on mechanisms for secretory and aggregatory responses of human granulocytes. Biochem Pharmacol. 1988 Sep 1;37(17):3237–3243. doi: 10.1016/0006-2952(88)90633-8. [DOI] [PubMed] [Google Scholar]
  23. Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984 Apr 19;308(5961):693–698. doi: 10.1038/308693a0. [DOI] [PubMed] [Google Scholar]
  24. Ozaki Y., Ohashi T., Niwa Y. Oxygen radical production by neutrophils from patients with bacterial infection and rheumatoid arthritis. Measurement of hydrogen peroxide may most accurately represent enhancement of oxygen radical production during infection. Inflammation. 1986 Jun;10(2):119–130. doi: 10.1007/BF00915994. [DOI] [PubMed] [Google Scholar]
  25. Palmblad J., Gyllenhammar H., Lindgren J. A., Malmsten C. L. Effects of leukotrienes and f-Met-Leu-Phe on oxidative metabolism of neutrophils and eosinophils. J Immunol. 1984 Jun;132(6):3041–3045. [PubMed] [Google Scholar]
  26. Phelps P., Stanislaw D. Polymorphonuclear leukocyte motility in vitro. I. Effect of pH, temperature, ethyl alcohol, and caffeine, using a modified Boyden chamber technic. Arthritis Rheum. 1969 Jun;12(3):181–188. doi: 10.1002/art.1780120304. [DOI] [PubMed] [Google Scholar]
  27. Schopf R. E., Trompeter M., Bork K., Morsches B. Effects of ethanol and acetaldehyde on phagocytic functions. Arch Dermatol Res. 1985;277(2):131–137. doi: 10.1007/BF00414111. [DOI] [PubMed] [Google Scholar]
  28. Schreiner A., Hopen G. Adhesion and locomotion of human leukocytes in vitro; importance of protein coating; effect of lidocain, ethanol and endotoxin. Acta Pathol Microbiol Scand C. 1979 Oct;87(5):333–340. [PubMed] [Google Scholar]
  29. Smith F. E., Palmer D. L. Alcoholism, infection and altered host defenses: a review of clinical and experimental observations. J Chronic Dis. 1976 Jan;29(1):35–49. doi: 10.1016/0021-9681(76)90066-7. [DOI] [PubMed] [Google Scholar]
  30. Spagnuolo P. J., MacGregor R. R. Acute thanol effect on chemotaxis and other components of host defense. J Lab Clin Med. 1975 Jul;86(1):24–31. [PubMed] [Google Scholar]
  31. Teisberg P. In vivo activation of C3 revealed by crossed immunoelectrophoresis as a parameter of immunological activity in disease. Clin Chim Acta. 1975 Jul 9;62(1):35–41. doi: 10.1016/0009-8981(75)90277-6. [DOI] [PubMed] [Google Scholar]
  32. Tennenberg S. D., Solomkin J. S. Neutrophil activation in sepsis. The relationship between fmet-leu-phe receptor mobilization and oxidative activity. Arch Surg. 1988 Feb;123(2):171–175. doi: 10.1001/archsurg.1988.01400260051005. [DOI] [PubMed] [Google Scholar]
  33. Thomsen P., Bjursten L. M., Ericson L. E. Joint fluid leukocyte activation by preformed immune complexes. Inflammation. 1986 Sep;10(3):243–256. doi: 10.1007/BF00916120. [DOI] [PubMed] [Google Scholar]
  34. Thomsen P., Bjursten L. M., Hansson H. A., Bagge U. Acute synovitis induced by preformed immune complexes. Scand J Rheumatol. 1986;15(2):134–142. doi: 10.3109/03009748609102079. [DOI] [PubMed] [Google Scholar]
  35. Thomsen P., Ericson L. E. Leukocyte migration in synovial tissue. Leukocyte distribution, orientation, and migratory pattern after immune complex deposition in rabbit knee joints. Am J Pathol. 1989 Jun;134(6):1213–1226. [PMC free article] [PubMed] [Google Scholar]
  36. Van Epps D. E., Garcia M. L. Enhancement of neutrophils function as a result of prior exposure to chemotactic factor. J Clin Invest. 1980 Aug;66(2):167–175. doi: 10.1172/JCI109841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wlodaver C. G., O'Neill K., MacGregor R. R. Ethanol inhibition of granulocyte adherence. Am J Med Sci. 1986 May;291(5):310–313. doi: 10.1097/00000441-198605000-00004. [DOI] [PubMed] [Google Scholar]
  38. Zimmerli W., Seligmann B., Gallin J. I. Exudation primes human and guinea pig neutrophils for subsequent responsiveness to the chemotactic peptide N-formylmethionylleucylphenylalanine and increases complement component C3bi receptor expression. J Clin Invest. 1986 Mar;77(3):925–933. doi: 10.1172/JCI112391. [DOI] [PMC free article] [PubMed] [Google Scholar]

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