Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1972 Nov;12(3):325–333.

Quantitative dinitrochlorobenzene contact sensitization in a normal population

W J Catalona, P T Taylor, P B Chretien
PMCID: PMC1553595  PMID: 4642908

Abstract

Using a quantitative method based on the sponteneous flare phenomenon, contact sensitization to DNCB was studied in 143 healthy volunteers between the ages of 20 and 80 yr. A spontaneous flare reaction occurred in 96·5% of subjects tested, regardless of age, and a correlation was demonstrated between the intensity of the primary response to DNCB and the threshold dose of DNCB required to elicit an anamnestic response. The results are in striking contrast to a 40% incidence of spontaneous flare reactions previously found in cancer patients using the same method. These findings show that this method of assaying reactivity to DNCB detects abnormalities of cell-mediated immunity not demonstrated by qualitative methods.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AISENBERG A. C. Studies on delayed hypersensitivity in Hodgkin's disease. J Clin Invest. 1962 Nov;41:1964–1970. doi: 10.1172/JCI104654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRENNER L. O., WAIFE S. O., WOHL M. G. The antibody response in old age. J Gerontol. 1951 Jul;6(3):229–232. doi: 10.1093/geronj/6.3.229. [DOI] [PubMed] [Google Scholar]
  3. Brown R. S., Haynes H. A., Foley H. T., Godwin H. A., Berard C. W., Carbone P. P. Hodgkin's disease. Immunologic, clinical, and histologic features of 50 untreated patients. Ann Intern Med. 1967 Aug;67(2):291–302. doi: 10.7326/0003-4819-67-2-291. [DOI] [PubMed] [Google Scholar]
  4. CONE L., UHR J. W. IMMUNOLOGICAL DEFICIENCY DISORDERS ASSOCIATED WITH CHRONIC LYMPHOCYTIC LEUKEMIA AND MULTIPLE MYELOMA. J Clin Invest. 1964 Dec;43:2241–2248. doi: 10.1172/JCI105098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Catalona W. J., Taylor P. T., Rabson A. S., Chretien P. B. A method for dinitrochlorobenzene contact sensitization. A clinicopathological study. N Engl J Med. 1972 Feb 24;286(8):399–402. doi: 10.1056/NEJM197202242860804. [DOI] [PubMed] [Google Scholar]
  6. Cheema A. R., Hersh E. M. Patient survival after chemotherapy and its relationship to in vitro lymphocyte blastogenesis. Cancer. 1971 Oct;28(4):851–855. doi: 10.1002/1097-0142(1971)28:4<851::aid-cncr2820280408>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  7. EPSTEIN W. L. Induction of allergic contact dermatitis in patients with the lymphoma-leukemia complex. J Invest Dermatol. 1958 Jan;30(1):39–40. doi: 10.1038/jid.1958.8. [DOI] [PubMed] [Google Scholar]
  8. Eilber F. R., Morton D. L. Impaired immunologic reactivity and recurrence following cancer surgery. Cancer. 1970 Feb;25(2):362–367. doi: 10.1002/1097-0142(197002)25:2<362::aid-cncr2820250213>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  9. Fox R. A., Scheuer P. J., Sherlock S., Sharma O., James D. G. Delayed hypersensitivity in primary biliary cirrhosis. Gut. 1968 Dec;9(6):729–729. [PubMed] [Google Scholar]
  10. GROSS L. IMMUNOLOGICAL DEFECT IN AGED POPULATION AND ITS RELATIONSHIP TO CANCER. Cancer. 1965 Feb;18:201–204. doi: 10.1002/1097-0142(196502)18:2<201::aid-cncr2820180211>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  11. Hersh E. M., Oppenheim J. J. Impaired in vitro lymphocyte transformation in Hodgkin's disease. N Engl J Med. 1965 Nov 4;273(19):1006–1012. doi: 10.1056/NEJM196511042731903. [DOI] [PubMed] [Google Scholar]
  12. Hersh E. M., Whitecar J. P., Jr, McCredie K. B., Bodey G. P., Sr, Freireich E. J. Chemotherapy, immunocompetence, immunosuppression and prognosis in acute leukemia. N Engl J Med. 1971 Nov;285(22):1211–1216. doi: 10.1056/NEJM197111252852201. [DOI] [PubMed] [Google Scholar]
  13. Hughes L. E., Mackay W. D. Suppression of the tuberculin response in malignant disease. Br Med J. 1965 Dec 4;2(5474):1346–1348. doi: 10.1136/bmj.2.5474.1346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jones J. V. Development of sensitivity oto dinitrochlorobenzene in patients with sarcoidosis. Clin Exp Immunol. 1967 Jul;2(4):477–487. [PMC free article] [PubMed] [Google Scholar]
  15. Krant M. J., Manskopf G., Brandrup C. S., Madoff M. A. Immunologic alterations in bronchogenic cancer. Sequential study. Cancer. 1968 Apr;21(4):623–631. doi: 10.1002/1097-0142(196804)21:4<623::aid-cncr2820210414>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  16. LAMB D., PILNEY F., KELLY W. D., GOOD R. A. A comparative study of the incidence of anergy in patients with carcinoma, leukemia, hodgkin's disease and other lymphomas. J Immunol. 1962 Oct;89:555–558. [PubMed] [Google Scholar]
  17. LEVIN A. G., MCDONOUGH E. F., Jr, MILLER D. G., SOUTHAM C. M. DELAYED HYPERSENSITIVITY RESPONSE TO DNFB IN SICK AND HEALTHY PERSONS. Ann N Y Acad Sci. 1964 Nov 30;120:400–409. doi: 10.1111/j.1749-6632.1964.tb34739.x. [DOI] [PubMed] [Google Scholar]
  18. ROSTENBERG A., Jr, MCCRANEY H. C., BLUEFARB S. M. Immunologic studies in the lymphoblastomas. II. The ability to develop an eczematous sensitization to a simple chemical and the ability to accept passive transfer antibody. J Invest Dermatol. 1956 Mar;26(3):209–216. [PubMed] [Google Scholar]
  19. STARR S., BERKOVICH S. EFFECTS OF MEASLES, GAMMA-GLOBULIN-MODIFIED MEASLES AND VACCINE MEASLES ON THE TUBERCULIN TEST. N Engl J Med. 1964 Feb 20;270:386–391. doi: 10.1056/NEJM196402202700802. [DOI] [PubMed] [Google Scholar]
  20. Sharma O. P., James D. G., Fox R. A. A correlation of in vivo delayed-type hypersensitivity with in vitro lymphocyte transformation in sarccidosis. Chest. 1971 Jul;60(1):35–37. doi: 10.1378/chest.60.1.35. [DOI] [PubMed] [Google Scholar]
  21. Solowey A. C., Rapaport F. T. Immunologic responses in cancer patients. Surg Gynecol Obstet. 1965 Oct;121(4):756–760. [PubMed] [Google Scholar]
  22. Turk J. L., Waters M. F. Cell-mediated immunity in patients with leprosy. Lancet. 1969 Aug 2;2(7614):243–246. doi: 10.1016/s0140-6736(69)90009-9. [DOI] [PubMed] [Google Scholar]
  23. Waldorf D. S., Willkens R. F., Decker J. L. Impaired delayed hypersensitivity in an aging population. Association with antinuclear reactivity and rheumatoid factor. JAMA. 1968 Mar 4;203(10):831–834. [PubMed] [Google Scholar]
  24. Walker J. G., Greaves M. F. Delayed hypersensitivity and lymphocyte transformation in Crohn's disease and proctocolitis. Gut. 1969 May;10(5):414–414. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES