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. 1972 Dec;12(4):489–496.

The formaldehyde stability of thymocyte immunogens

B A Bradley, A D Barnes
PMCID: PMC1553601  PMID: 4568151

Abstract

The differential effect of formaldehyde on mouse thymic and erythrocyte immunogens is studied using a simple technique. Changes are reflected in the antibody-independent properties of rabbit antisera to these treated cells. Taken as a whole all properties are quantitatively reduced in fifteen antisera but there are some notable exceptions. The five antisera with the highest lymphocytotoxic titres, having titres equal with fresh thymocyte antisera, show detectable levels of immunosuppression. This falls significantly below the level achieved with antisera to fresh thymocytes to suggest that some relevant immunogens are susceptible and some resistant to formaldehyde; the latter alone perhaps determine lymphocytotoxicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRENT L., MEDAWAR P. B., RUSZKIEWICZ M. Serological methods in the study of transplantation antigens. Br J Exp Pathol. 1961 Oct;42:464–477. [PMC free article] [PubMed] [Google Scholar]
  2. Barnes A. D., Bradley B. A. A synergistic effect of pooling individual antithymocyte sera. Transplantation. 1971 Oct;12(4):324–327. doi: 10.1097/00007890-197110000-00017. [DOI] [PubMed] [Google Scholar]
  3. Barnes A. D., Crosier C. Effect of antilymphocyte serum on the survival of ovary allografts in the mouse. Nature. 1969 Sep 6;223(5210):1059–1060. doi: 10.1038/2231059a0. [DOI] [PubMed] [Google Scholar]
  4. Bradley B. A., Barnes A. D. The heterophile reaction between anti-mouse thymocyte sera and sheep erythrocytes. Tissue Antigens. 1972;2(2):128–138. doi: 10.1111/j.1399-0039.1972.tb00127.x. [DOI] [PubMed] [Google Scholar]
  5. Greaves M. F., Tursi A., Playfair J. H., Torrigiani G., Zamir R., Roitt I. M. Immunosuppressive potency and in-vitro activity of antilymphocyte globulin. Lancet. 1969 Jan 11;1(7585):68–72. doi: 10.1016/s0140-6736(69)91089-7. [DOI] [PubMed] [Google Scholar]
  6. HEARD D. H., SEAMAN G. V. The action of lower aldehydes on the human erythrocyte. Biochim Biophys Acta. 1961 Oct 28;53:366–374. doi: 10.1016/0006-3002(61)90448-6. [DOI] [PubMed] [Google Scholar]
  7. Habeeb A. F. A study of the antigenicity of formaldehyde- and glutaraldehyde-treated bovine serum albumin and ovalbumin-bovine serum albumin conjugate. J Immunol. 1969 Feb;102(2):457–465. [PubMed] [Google Scholar]
  8. Hewitt L. F. Note on the possible mechanism of diphtheria toxoid formation. Biochem J. 1930;24(4):983–992. doi: 10.1042/bj0240983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jooste S. V., Lance E. M., Levey R. H., Medawar P. B., Ruszkiewicz M., Sharman R., Taub R. N. Notes on the preparation and assay of anti-lymphocytic serum for use in mice. Immunology. 1968 Nov;15(5):697–705. [PMC free article] [PubMed] [Google Scholar]
  10. Lance E. M., Ford P. J., Ruszkiewicz M. The use of subcellular fractions to raise anti-lymphocytic serum. Immunology. 1968 Oct;15(4):571–580. [PMC free article] [PubMed] [Google Scholar]
  11. Levey R. H., Medawar P. B. Nature and mode of action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1130–1137. doi: 10.1073/pnas.56.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MOSKOWITZ M., CARB S. Surface alteration and the agglutinability of red cells. Nature. 1957 Nov 16;180(4594):1049–1050. doi: 10.1038/1801049a0. [DOI] [PubMed] [Google Scholar]
  13. Pass F., Marcus D. M. Specificity of rabbit antibody to formaldehyde treated rabbit serum albumin. J Invest Dermatol. 1970 Jan;54(1):32–36. doi: 10.1111/1523-1747.ep12551498. [DOI] [PubMed] [Google Scholar]

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