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letter
. 1972 Jun;11(2):305–309.

The mechanism of antigen-induced electrophoretic mobility reduction of guinea-pig macrophages

E A Caspary
PMCID: PMC1553633  PMID: 5040311

Abstract

The interaction of antigen with sensitized lymphocytes produces a protein substance capable of reducing the electrophoretic mobility of guinea-pig peritoneal macrophages. Reaction with the protein slowing factor also involves some vital synthetic activities of the macrophage. A critical number of lymphocytes is required to initiate the complete reaction suggesting that above this threshold recruitment may take place. It is suggested that the mechanism of action is a close parallel to that of macrophage migration inhibition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom B. R., Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science. 1966 Jul 1;153(3731):80–82. doi: 10.1126/science.153.3731.80. [DOI] [PubMed] [Google Scholar]
  2. COULSON A. S., CHALMERS D. G. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD. Lancet. 1964 Feb 29;1(7331):468–469. doi: 10.1016/s0140-6736(64)90799-8. [DOI] [PubMed] [Google Scholar]
  3. Caspary E. A., Field E. J. Sensitization of blood lymphocytes to possible antigens in neurological disease. Eur Neurol. 1970;4(5):257–266. doi: 10.1159/000114027. [DOI] [PubMed] [Google Scholar]
  4. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  5. David J. R. Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A. 1966 Jul;56(1):72–77. doi: 10.1073/pnas.56.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diengdoh J. V., Turk J. L. Electrophoretic mobility of guinea pig peritoneal exudate cells in hypersensitivity reactions. Int Arch Allergy Appl Immunol. 1968;34(3):297–302. doi: 10.1159/000230121. [DOI] [PubMed] [Google Scholar]
  7. Field E. J., Caspary E. A. Demonstration of sensitized lymphocytes in blood. J Clin Pathol. 1971 Mar;24(2):179–181. doi: 10.1136/jcp.24.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Field E. J., Caspary E. A., Hall R., Clark F. Circulating sensitised lymphocytes in Graves' disease. Observations on its pathogenesis. Lancet. 1970 May 30;1(7657):1144–1147. doi: 10.1016/s0140-6736(70)91217-1. [DOI] [PubMed] [Google Scholar]
  9. Field E. J., Caspary E. A. Lymphocyte sensitisation: an in-vitro test for cancer? Lancet. 1970 Dec 26;2(7687):1337–1341. doi: 10.1016/s0140-6736(70)92361-5. [DOI] [PubMed] [Google Scholar]
  10. Hartveit F., Cater D. B., Mehrishi J. N. Changes in the electrophoretic mobility of mouse lymphocytes, thymocytes, macrophages and tumour cells following immunisation. Br J Exp Pathol. 1968 Dec;49(6):634–647. [PMC free article] [PubMed] [Google Scholar]
  11. Hughes D., Caspary E. A. Lymphocyte transformation in vitro measured by tritiated thymidine uptake. I. Lymphocyte culture techniques. Int Arch Allergy Appl Immunol. 1970;37(5):506–531. doi: 10.1159/000230240. [DOI] [PubMed] [Google Scholar]
  12. Knowles M., Hughes D. A technique for rapid isolation of macrophages from guinea-pig peritoneal exudates. J Clin Pathol. 1970 Dec;23(9):824–825. doi: 10.1136/jcp.23.9.824. [DOI] [PMC free article] [PubMed] [Google Scholar]

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