Abstract
A whole blood culture technique has been employed to quantitatively evaluate lymphocyte reactivity of small laboratory animals including mice, rats, guinea-pigs and rabbits. This simplified method has been used to study the blastogenic response to phytohaemagglutinin, concanavalin A, pokeweed mitogen and staphy-lococcal filtrate. Tuberculin-induced lymphocyte transformation responses have been studied in serial experiments using sensitized animals. The results of this preliminary study demonstrate that the whole blood method has numerous advantages over the conventional techniques employing leucocyte concentrates. In almost all instances the degree of lymphocyte reactivity as measured by 3H-thymidine incorporation closely approximated or exceeded previously reported results using the standard culture method.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hughes D., Caspary E. A. Lymphocyte transformation in vitro measured by tritiated thymidine uptake. I. Lymphocyte culture techniques. Int Arch Allergy Appl Immunol. 1970;37(5):506–531. doi: 10.1159/000230240. [DOI] [PubMed] [Google Scholar]
- Junge U., Hoekstra J., Wolfe L., Deinhardt F. Microtechnique for quantitative evaluation of in vitro lymphocyte transformation. Clin Exp Immunol. 1970 Sep;7(3):431–437. [PMC free article] [PubMed] [Google Scholar]
- Metcalf W. K. Some experiments on the phytohaemagglutinin culture of leucocytes from rats and other mammals. Exp Cell Res. 1965 Dec;40(3):490–498. doi: 10.1016/0014-4827(65)90228-4. [DOI] [PubMed] [Google Scholar]
- Mumford D. M., Barsales P. B., Ball K. D., Gordon H. L. Microlymphocyte transformation studies with seminal antigen. I. Technique and patterns of responsiveness to autologous and allogeneic semen from normal and infertile male subjects. J Urol. 1971 Jun;105(6):858–862. doi: 10.1016/s0022-5347(17)61646-x. [DOI] [PubMed] [Google Scholar]
- Oppenheim J. J., Wolstencroft R. A., Gell P. G. Delayed hypersensitivity in the guinea-pig to a protein-hapten conjugate and its relationship to in vitro transformation of lymph node, spleen, thymus and peripheral blood lymphocytes. Immunology. 1967 Jan;12(1):89–102. [PMC free article] [PubMed] [Google Scholar]
- Schellekens P. T., Eijsvoogel V. P. Lymphocyte transformation in vitro. I. Tissue culture conditions and quantitative measurements. Clin Exp Immunol. 1968 Jul;3(6):571–584. [PMC free article] [PubMed] [Google Scholar]