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. 1972 Aug;11(4):595–603.

Binding of 125I myelin basic protein by serum and cerebrospinal fluid

Vanda Lennon, I R Mackay
PMCID: PMC1553691  PMID: 4117459

Abstract

A sensitive gel filtration redioimmunoassay was used to test for antibodies to the basic protein of myelin, the antigen of experimental autoimmune encephalomyelitis, in serum and cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) and other diseases. Sera and CSF from patients with MS gave results similar to those for controls including subjects with various neurological diseases. Binding of 125I-basic protein by seven-fold concentrated CSF was shown to be due to α-globulin. Free basic protein, as a possible auto-immunogen, was sought in the serum and seven-fold concentrated CSF of patients with MS and controls by competitive inhibition in the radioimmunoassay, but none was demonstrable.

If an immunological mechanism is to be invoked in the initiation of destruction of the basic protein of myelin in MS, then either pathogenic `antibody' must be absorbed in vivo to the target antigen in the central nervous system or the initiating events must be mediated entirely by sensitized lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alvord E. C., Jr, Shaw C. M., Lisak R. P., Falk G. A., Kies M. W. Relationships between antibodies and experimental allergic encephalomyelitis. V. Antibodies and delayed hypersensitivity in production and prevention of experimental allergic encephalomyelitis. Int Arch Allergy Appl Immunol. 1970;38(4):403–412. doi: 10.1159/000230292. [DOI] [PubMed] [Google Scholar]
  2. BORNSTEIN M. B. A tissue-culture approach to demyelinative disorders. Natl Cancer Inst Monogr. 1963 Mar;11:197–214. [PubMed] [Google Scholar]
  3. BORNSTEIN M. B., CRAIN S. M. FUNCTIONAL STUDIES OF CULTURED BRAIN TISSUES AS RELATED TO "DEMYELINATIVE DISORDERS". Science. 1965 May 28;148(3674):1242–1244. doi: 10.1126/science.148.3674.1242. [DOI] [PubMed] [Google Scholar]
  4. Bartfeld H., Atoynatan T. In vitro delayed (cellular) hypersensitivity in multiple sclerosis to central nervous system antigens. Int Arch Allergy Appl Immunol. 1970;39(4):361–367. doi: 10.1159/000230364. [DOI] [PubMed] [Google Scholar]
  5. Carnegie P. R. Properties, structure and possible neuroreceptor role of the encephalitogenic protein of human brain. Nature. 1971 Jan 1;229(5279):25–28. doi: 10.1038/229025a0. [DOI] [PubMed] [Google Scholar]
  6. Cerf J. A., Carels G. Multiple sclerosis: serum factor producing reversible alterations in bioelectric responses. Science. 1966 May 20;152(3725):1066–1068. doi: 10.1126/science.152.3725.1066. [DOI] [PubMed] [Google Scholar]
  7. Einstein E. R., Dalal K. B., Csejtey J. Increased protease activity and changes in basic proteins and lipids in multiple sclerosis plaques. J Neurol Sci. 1970 Aug;11(2):109–121. doi: 10.1016/0022-510x(70)90121-8. [DOI] [PubMed] [Google Scholar]
  8. Falk G. A., Heinze R. G., Kies M. W., Alvord E. C., Jr Skin-fixing antibody in experimental allergic encephalomyelitis. J Immunol. 1968 Feb;100(2):321–328. [PubMed] [Google Scholar]
  9. Hallpike J. F., Adams C. W., Bayliss O. B. Histochemistry of myelin. XI. Loss of basic protein in early myelin breakdown and multiple sclerosis plaques. Histochem J. 1970 Jul;2(4):323–328. doi: 10.1007/BF01005000. [DOI] [PubMed] [Google Scholar]
  10. Herndon R. M., Johnson M. A method for the electron microscopic study of cerebrospinal fluid sediment. J Neuropathol Exp Neurol. 1970 Apr;29(2):320–330. [PubMed] [Google Scholar]
  11. Lennon V. A., Carnegie P. R. Immunopharmacological disease: a break in tolerance to receptor sites. Lancet. 1971 Mar 27;1(7700):630–633. doi: 10.1016/s0140-6736(71)91557-1. [DOI] [PubMed] [Google Scholar]
  12. Lennon V. A., Whittingham S., Carnegie P. R., McPherson T. A., Mackay I. R. Detection of antibodies to the basic protein of human myelin by radioimmunoassay and immunofluorescence. J Immunol. 1971 Jul;107(1):56–62. [PubMed] [Google Scholar]
  13. Lennon V. A., Wilks A. V., Carnegie P. R. Immunologic properties of the main encephalitogenic peptide from the basic protein of human myelin. J Immunol. 1970 Nov;105(5):1223–1230. [PubMed] [Google Scholar]
  14. Link H., Zettervall O. Multiple sclerosis: disturbed kappa: lambda chain ratio of immunoglobulin G in cerebrospinal fluid. Clin Exp Immunol. 1970 Mar;6(3):435–438. [PMC free article] [PubMed] [Google Scholar]
  15. Lisak R. P., Heinze R. G., Falk G. A., Kies M. W. Search for anti-encephalitogen antibody in human demyelinative diseases. Neurology. 1968 Jan;18(1 Pt 2):122–128. doi: 10.1212/wnl.18.1_part_2.122. [DOI] [PubMed] [Google Scholar]
  16. McPherson T. A., Carnegie P. R. Automated sample application for gel filtration. Anal Biochem. 1969 Aug;30(2):306–308. doi: 10.1016/0003-2697(69)90407-2. [DOI] [PubMed] [Google Scholar]
  17. McPherson T. A., Carnegie P. R. Radioimmunoassay with gel filtration for detecting antibody to basic proteins of myelin. J Lab Clin Med. 1968 Nov;72(5):824–831. [PubMed] [Google Scholar]
  18. McPherson T. A., Marchalonis J. J., Lennon V. Binding of encephalitogenic basic protein by serum alpha-globulins. Immunology. 1970 Dec;19(6):929–933. [PMC free article] [PubMed] [Google Scholar]
  19. Rocklin R. E., Sheremata W. A., Feldman R. G., Kies M. W., David J. R. The Guillain-Barré syndrome and multiple sclerosis. In vitro cellular responses to nervous-tissue antigens. N Engl J Med. 1971 Apr 15;284(15):803–808. doi: 10.1056/NEJM197104152841501. [DOI] [PubMed] [Google Scholar]
  20. Sandberg-Wollheim M., Zettervall O., Müller R. In vitro synthesis of IgG by cells from the cerebrospinal fluid in a patient with multiple sclerosis. Clin Exp Immunol. 1969 Apr;4(4):401–405. [PMC free article] [PubMed] [Google Scholar]
  21. Seil F. J., Falk G. A., Kies M. W., Alvord E. C., Jr The in vitro demyelinating activity of sera from guinea pigs sensitized with whole CNS and with purified encephalitogen. Exp Neurol. 1968 Dec;22(4):545–555. doi: 10.1016/0014-4886(68)90148-9. [DOI] [PubMed] [Google Scholar]
  22. Tourtellotte W. On cerebrospinal fluid immunoglobulin-G (IgG) quotients in multiple sclerosis and other diseases. A review and a new formula to estimate the amount of IgG synthesized per day by the central nervous system. J Neurol Sci. 1970 Mar;10(3):279–304. doi: 10.1016/0022-510x(70)90156-5. [DOI] [PubMed] [Google Scholar]

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