Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1973 Feb;13(2):263–278.

The antigenic and immunosuppressive properties of normal and antilymphocytic equine IgG subfractions

R A Allardyce, N F Anderson, J P Vaerman, K James
PMCID: PMC1553709  PMID: 4120853

Abstract

The antigenic and immunosuppressive properties of normal and antilympho-cytic equine globulin subfractions were investigated in the rat model in an attempt to increase the efficacy of prolonged ALG therapy by limiting the immunogenic stimulus of `inactive' subfractions.

Chromatographic separation of equine IgG components yielded an electro-phoretically slow gamma 2 fraction consisting of IgG2a and IgG2b and a more heterogeneous fast gamma 1 subfraction. Immunosuppression resulting from the administration of isolated subfractions was measured by the response to alum-BSA and skin allograft survival. Antigenicity was determined by a variety of immunological procedures. The immunosuppressive character of the ALG was confined to the gamma 2 fraction, however this fraction also proved antigenic in our system.

The administration of normal equine IgG subfractions in combination with Freund's complete adjuvant resulted in the demonstration of antigenic differences between the fast and slow IgG components.

Full text

PDF
263

Images in this article

267Fig. 1
on p.267
269Fig. 2
on p.269

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ameniya H., Kashiwagi N., Putnam C. W., Starzl T. E. Cross-reactivity studies of horse, goat and rabbit anti-lymphocyte globulin. Clin Exp Immunol. 1970 Feb;6(2):279–289. [PMC free article] [PubMed] [Google Scholar]
  2. Balner H., Yron I., Dersjant H., Zaalberg O., Betel I. ALG treatment of Rhesus monkeys. Attempts to reduce complications by the induction of tolerance. Transplantation. 1970 Nov;10(5):416–424. [PubMed] [Google Scholar]
  3. Betel I., Appelman A. W., Balner H. The localization of the immunosuppressive activity in horse antimonkey lymphocyte sera. Transplantation. 1970 Apr;9(4):431–436. [PubMed] [Google Scholar]
  4. Busch G. J., Birtch A. G., Lukl P., Jr, Kobayashi K., Galvanek E. G., Carpenter C. B. Human renal allografts. Glomerular deposits of horse immunoglobulin G and nephritis following administration of antilymphocyte globulin. Hum Pathol. 1971 Jun;2(2):299–308. doi: 10.1016/s0046-8177(71)80038-2. [DOI] [PubMed] [Google Scholar]
  5. DRESSER D. W., GOWLAND G. IMMUNOLOGICAL PARALYSIS INDUCED IN ADULT RABBITS BY SMALL AMOUNTS OF A PROTEIN ANTIGEN. Nature. 1964 Aug 15;203:733–736. doi: 10.1038/203733a0. [DOI] [PubMed] [Google Scholar]
  6. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  7. GENUTH S., FROHMAN L. A., LEBOVITZ H. E. A RADIOIMMUNOLOGICAL ASSAY METHOD FOR INSULIN USING INSULIN-125I AND GEL FILTRATION. J Clin Endocrinol Metab. 1965 Aug;25:1043–1049. doi: 10.1210/jcem-25-8-1043. [DOI] [PubMed] [Google Scholar]
  8. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  9. James K. Anti-lymphocytic antibody--a review. Clin Exp Immunol. 1967 Nov;2(6):615–631. [PMC free article] [PubMed] [Google Scholar]
  10. James K., James V. S., Pullar D. M., Wood A., Logan E. In vitro studies on the heterogeneity of equine antibodies to human lymphoid tissue. Immunochemistry. 1969 Sep;6(5):659–680. doi: 10.1016/0019-2791(67)90131-0. [DOI] [PubMed] [Google Scholar]
  11. James K., Pullar D. M., Brydon J. The immunosuppressive properties of sub-fractions of antilymphocytic IgG of equine origin. Immunology. 1971 Aug;21(2):247–257. [PMC free article] [PubMed] [Google Scholar]
  12. James K., Pullar D. M., James V. S., Wood A., Epps H. B., Rahr L. The development and distribution of antilymphocytic and other antibodies in horses immunized with human lymphoid antigens. Transplantation. 1970 Sep;10(3):208–226. doi: 10.1097/00007890-197009000-00002. [DOI] [PubMed] [Google Scholar]
  13. James K. The preparation and properties of anti-lymphocytic sera. Prog Surg. 1969;7:140–217. doi: 10.1159/000386301. [DOI] [PubMed] [Google Scholar]
  14. Kashiwagi N., Brantigan C. O., Brettschneider L., Groth C. G., Starzl T. E. Clinical reactions and serologic changes after the administration of heterologous antilymphocyte globulin to human recipients of renal homografts. Ann Intern Med. 1968 Feb;68(2):275–286. doi: 10.7326/0003-4819-68-2-275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kashiwagi N., Sherer D., Townsend C. M., Jacobs R., Ono K., Kapur B., Starzl T. E. Antibody localization in horse, rabbit, and goat antilymphocyte sera. Surgery. 1970 May;67(5):789–798. [PMC free article] [PubMed] [Google Scholar]
  16. Klinman N. R., Rockey J. H., Frauenberger G., Karush F. Equine anti-hapten antibody. 3. The comparative properties of gamma G- and gammaA-antibodies. J Immunol. 1966 Apr;96(4):587–595. [PubMed] [Google Scholar]
  17. Moberg A. W., Gewurz H., Simmons R. L., Najarian J. S. Desensitisation to horse-globulin before immunosuppressive treatment. Lancet. 1970 Jul 25;2(7665):214–214. doi: 10.1016/s0140-6736(70)92569-9. [DOI] [PubMed] [Google Scholar]
  18. Moberg A. W., Gewurz H., Simmons R. L., Najarian J. S. Pretreatment with deaggregated horse gamma-globulin. Failure to enhance tolerance to horse antilymphoblast globulin in man. Transplantation. 1970 Oct;10(4):344–346. [PubMed] [Google Scholar]
  19. Moberg A. W., Gewurz H., Simmons R. L., Najarian J. S. Pretreatment with deaggregated horse gamma-globulin. Failure to enhance tolerance to horse antilymphoblast globulin in man. Transplantation. 1970 Oct;10(4):344–346. [PubMed] [Google Scholar]
  20. Müller-Eberhard H. J. Chemistry and reaction mechanisms of complement. Adv Immunol. 1968;8:1–80. doi: 10.1016/s0065-2776(08)60464-2. [DOI] [PubMed] [Google Scholar]
  21. Najarian J. S., Simmons R. L., Gewurz H., Moberg A., Merkel F., Moore G. E. Anti-serum to cultured human lymphoblasts: preparation, purification and immunosuppressive properties in man. Ann Surg. 1969 Oct;170(4):617–632. doi: 10.1097/00000658-196910000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Newcomb R. W., Ishizaka K. Human diphtheria antitoxin in immunoglobulin classes IgG and IgA. J Immunol. 1967 Jul;99(1):40–48. [PubMed] [Google Scholar]
  23. Perper R. J., Monovich R. E., Van Gorder T. J. Analysis of the biological activity of antilymphocyte serum. 3. Concentration of immunosuppressive activity in one IgG subfraction and its inhibition by another. Immunology. 1971 Dec;21(6):883–902. [PMC free article] [PubMed] [Google Scholar]
  24. ROCKEY J. H., KLINMAN N. R., KARUSH F. EQUINE ANTIHAPTEN ANTIBODY. I. 7S BETA-2A- AND 1OS GAMMA-1- GLOBULIN COMPONENTS OF PURIFIED ANTI-BETA-LACTOSIDE ANTIBODY. J Exp Med. 1964 Oct 1;120:589–609. doi: 10.1084/jem.120.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sanders J. H., Jr A microdroplet assay for immune adherence. Clin Exp Immunol. 1972 Jan;10(1):191–197. [PMC free article] [PubMed] [Google Scholar]
  26. Starzl T. E., Penn I., Brettschneider L., Ono K., Kashiwagi N. Selected topics on antilymphocyte serum. Fed Proc. 1970 Jan-Feb;29(1):186–190. [PMC free article] [PubMed] [Google Scholar]
  27. Stockman G. D., Trentin J. J. Cyclophosphamide-induced tolerance to equine globulin and to equine-anti-mouse-thymocyte globulin in adult mice. I. Studies on antigen and drug requirements. J Immunol. 1972 Jan;108(1):112–118. [PubMed] [Google Scholar]
  28. Vaerman J. P., Querinjean P., Heremans J. F. Studies on the IgA system of the horse. Immunology. 1971 Sep;21(3):443–454. [PMC free article] [PubMed] [Google Scholar]
  29. Weksler M. E., Bull G., Schwartz G. H., Stenzel K. H., Rubin A. L. Immunologic responses of graft recipients to antilymphocyte globulin: effect of prior treatment with aggregate-free gamma globulin. J Clin Invest. 1970 Aug;49(8):1589–1595. doi: 10.1172/JCI106376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wolf R. E., Remmers A. R., Jr, Sarles H. E., Fish J. C., Lindley J. D., Murillo J. A., Ritzmann S. E. Equine anti-human lymphocyte serum and derivatives: purification and assays. J Reticuloendothel Soc. 1971 Sep;10(3):283–292. [PubMed] [Google Scholar]
  31. Wolf R. E., Remmers A. R., Jr, Sarles H. E., Fish J. C., Lindley J. D., Vyvial T. M., Ritzmann S. E. The immunogenic components of equine anti-human lymphocyte lobulin in renal allograft recipients. Clin Exp Immunol. 1972 Feb;10(2):235–241. [PMC free article] [PubMed] [Google Scholar]
  32. Woodruff M. F., Reid B. L., James K. Quantitative studies with antilymphocytic antibody. Nature. 1967 Nov 25;216(5117):758–762. doi: 10.1038/216758a0. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES