Skip to main content
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1973 Feb;13(2):327–333.

The effect of temperature and time of incubation in the preparation of antigens for detection of virus-specific IgM antibody

Margaret Haire, B McC Adair, K B Fraser
PMCID: PMC1553711  PMID: 4571214

Abstract

The success of immunofluorescent staining with virus-specific IgM varies with the temperature of incubation of virus-infected cells, as well as with cell density. Absence of intracellular staining by IgM can be due to blocking at the cell membrane or to the physical effect of cell confluence which reduces the contrast of surface staining.

Full text

PDF
327

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Haire M., Hadden D. S. Immunoglobulin responses in rubella and its complications. Br Med J. 1970 Jul 18;3(5715):130–132. doi: 10.1136/bmj.3.5715.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Haire M., Hadden D. S. Rapid diagnosis of rubella by demonstrating rubella-specific IgM antibodies in ther serum by indirect immunofluorescence. J Med Microbiol. 1972 May;5(2):237–242. doi: 10.1099/00222615-5-2-237. [DOI] [PubMed] [Google Scholar]
  3. MAYOR H. D., JORDAN L. E. Formation of poliovirus in monkey kidney tissue culture cells. Virology. 1962 Mar;16:325–333. doi: 10.1016/0042-6822(62)90254-4. [DOI] [PubMed] [Google Scholar]
  4. Millar J. H., Fraser K. B., Haire M., Connolly J. H., Shirodaria P. V., Hadden D. S. Immunoglobulin M specific for measles and mumps in multiple sclerosis. Br Med J. 1971 May 15;2(5758):378–380. doi: 10.1136/bmj.2.5758.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. RAPP F., GORDON I., BAKER R. F. Observations of measles virus infection of cultured human cells. I. A study of development and spread of virus antigen by means of immunofluorescence. J Biophys Biochem Cytol. 1960 Feb;7:43–48. doi: 10.1083/jcb.7.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES