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. 1973 Jun;14(2):219–226.

Circulating immune complexes in inflammatory bowel disease

D P Jewell, I C M MacLennan
PMCID: PMC1553792  PMID: 4197968

Abstract

Evidence for circulating immune complexes in sera of patients with ulcerative colitis or Crohn's disease is described. Such sera produced significantly greater inhibition of antibody-induced cytotoxicity mediated by lymphocytes than did sera from normal control subjects. Inhibitory activity of patients' sera showed a positive correlation with severity of disease, the height of the ESR and, in the case of ulcerative colitis, with the severity of inflammation as seen at sigmoidoscopy. Using gel filtration on Sepharose 6B, inhibitory activity was found in fractions of higher molecular weight than monomeric IgG but of lower molecular weight than IgM. These fractions were shown to contain IgG measured by immunodiffusion. Evidence that inhibition is not due to aggregated IgG molecules is provided by the fact that no correlation was found between inhibitory activity and total IgG concentration. A possible role of immune complexes in the pathogenesis of ulcerative colitis and Crohn's disease is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., SEBESTYEN M., COOPER N. S. The clearance of antigen antibody complexes from the blood by the reticuloendothelial system. J Immunol. 1959 Feb;82(2):131–137. [PubMed] [Google Scholar]
  2. Barnett I. G., MacLennan I. C. Inhibitory effect of rheumatoid sera on cell damage by lymphocytes. Ann Rheum Dis. 1972 Sep;31(5):425–425. doi: 10.1136/ard.31.5.425-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COULSON A. S., CHALMERS D. G. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD. Lancet. 1964 Feb 29;1(7331):468–469. doi: 10.1016/s0140-6736(64)90799-8. [DOI] [PubMed] [Google Scholar]
  4. Henson P. M. The adherence of leucocytes and platelets induced by fixed IgG antibody or complement. Immunology. 1969 Jan;16(1):107–121. [PMC free article] [PubMed] [Google Scholar]
  5. Huber H., Polley M. J., Linscott W. D., Fudenberg H. H., Müller-Eberhard H. J. Human monocytes: distinct receptor sites for the third component of complement and for immunoglobulin G. Science. 1968 Dec 13;162(3859):1281–1283. doi: 10.1126/science.162.3859.1281. [DOI] [PubMed] [Google Scholar]
  6. Hyslop N. E., Jr, Dourmashkin R. R., Green N. M., Porter R. R. The fixation of complement and the activated first component (C1) of complement by complexes formed between antibody and divalent hapten. J Exp Med. 1970 Apr 1;131(4):783–802. doi: 10.1084/jem.131.4.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KOFFLER D., MINKOWITZ S., ROTHMAN W., GARLOCK J. Immunocytochemical studies in ulcerative colitis and regional ileitis. Am J Pathol. 1962 Dec;41:733–745. [PMC free article] [PubMed] [Google Scholar]
  8. KUNKEL H. G., MULLER-EBERHARD H. J., FUDENBERG H. H., TOMASI T. B. Gamma globulin complexes in rheumatoid arthritis and certain other conditions. J Clin Invest. 1961 Jan;40:117–129. doi: 10.1172/JCI104224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MOELLER E. CONTACT-INDUCED CYTOTOXICITY BY LYMPHOID CELLS CONTAINING FOREIGN ISOANTIGENS. Science. 1965 Feb 19;147(3660):873–879. doi: 10.1126/science.147.3660.873. [DOI] [PubMed] [Google Scholar]
  10. MacLennan I. C. Competition for receptors for immunoglobulin on cytotoxic lymphocytes. Clin Exp Immunol. 1972 Feb;10(2):275–283. [PMC free article] [PubMed] [Google Scholar]
  11. MacLennan I. C., Howard A. Evidence for correlation between the antigenic specificity and charge of human IgG. A study of antibody-inducing lymphocyte mediated cell damage. Immunology. 1972 Jun;22(6):1043–1049. [PMC free article] [PubMed] [Google Scholar]
  12. MacLennan I. C., Loewi G., Howard A. A human serum immunoglobulin with specificity for certain homologous target cells, which induces target cell damage by normal human lymphocytes. Immunology. 1969 Dec;17(6):897–910. [PMC free article] [PubMed] [Google Scholar]
  13. Mannik M., Arend M. P., Hall A. P., Gilliland B. C. Studies on antigen-antibody complexes. I. Elimination of soluble complexes from rabbit circulation. J Exp Med. 1971 Apr 1;133(4):713–739. doi: 10.1084/jem.133.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Monteiro E., Fossey J., Shiner M., Drasar B. S., Allison A. C. Antibacterial antibodies in rectal and colonic mucosa in ulcerative colitis. Lancet. 1971 Feb 6;1(7693):249–250. doi: 10.1016/s0140-6736(71)90997-4. [DOI] [PubMed] [Google Scholar]
  15. Perlmann P., Perlmann H., Biberfeld P. Specifically cytotoxic lymphocytes produced by preincubation with antibody-complexed target cells. J Immunol. 1972 Feb;108(2):558–561. [PubMed] [Google Scholar]

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