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. 1973 Aug;14(4):481–490.

Surface immunoglobulins of leukaemic cells

A K Foulis, A J Cochran, J R Anderson
PMCID: PMC1553812  PMID: 4518234

Abstract

We have examined leukaemia cells from twenty-eight patients for the presence of surface immunoglobulins by a migration-inhibition technique. Immunoglobulins were identified on the cells of fourteen out of nineteen patients with chronic lymphatic leukaemia but were not present on cells from patients with chronic myeloid leukaemia or acute leukaemia. The results obtained with the migration technique were confirmed by identification of immunoglobulins in eluates and lysates of the leukaemia cells. Serial elution and lysis studies indicated that in some cases the immunoglobulins were produced by the leukaemia cells at a low but steady rate, possibly indicating a relationship between those cells and `B' cells. In other cases the immunoglobulins were readily removed from the cells and were present only in the initial eluates from the cells, suggesting that they had been adsorbed and might possibly be specific antibody. The immunoglobulin negative chronic lymphatic leukaemia cells may be related to `T' cells, a possibility supported by a high level of phytohaemagglutinin induced transformation in one case examined by this technique.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. L., Bach F. H., Joo P. Leukemia-associated antigens in the mixed leukocyte culture test. Science. 1969 Dec 19;166(3912):1520–1522. doi: 10.1126/science.166.3912.1520. [DOI] [PubMed] [Google Scholar]
  2. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bouroncle B. A., Clausen K. P., Aschenbrand J. F. Studies of the delayed response of phytohemagglutinin (PHA) stimulated lymphocytes in 25 chronic lymphatic leukemia patients before and during therapy. Blood. 1969 Aug;34(2):166–178. [PubMed] [Google Scholar]
  4. Brain P., Gordon J., Willetts W. A. Rosette formation by peripheral lymphocytes. Clin Exp Immunol. 1970 May;6(5):681–688. [PMC free article] [PubMed] [Google Scholar]
  5. Catovsky D., Tripp E., Hoffbrand A. V., König E., Cohnen G., Brittinger G., Douglas S. D., Fröland S. S., Stavem P. Response to phytohaemagglutinin and pokeweed mitogen in chronic lymphocytic leukemia. Lancet. 1972 Apr 8;1(7754):794–796. [PubMed] [Google Scholar]
  6. Cochran A. J., Klein E., Keissling R. Effect of immune factors on the motility of lymphoma cells. J Natl Cancer Inst. 1972 Jun;48(6):1657–1661. [PubMed] [Google Scholar]
  7. Cochran A. J. Tumour cell migration. Rev Eur Etud Clin Biol. 1971 Jan-Feb;16(1):44–47. [PubMed] [Google Scholar]
  8. Cone R. E., Marchalonis J. J., Rolley R. T. Lymphocyte membrane dynamics. Metabolic release of cell surface proteins. J Exp Med. 1971 Dec 1;134(6):1373–1384. doi: 10.1084/jem.134.6.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fridman W. H., Kourilsky F. M. Stimulation of lymphocytes by autologous leukaemic cells in acute leukaemia. Nature. 1969 Oct 18;224(5216):277–279. doi: 10.1038/224277a0. [DOI] [PubMed] [Google Scholar]
  10. Grey H. M., Rabellino E., Pirofsky B. Immunoglobulins on the surface of lymphocytes. IV. Distribution in hypogammaglobulinemia, cellular immune deficiency, and chronic lymphatic leukemia. J Clin Invest. 1971 Nov;50(11):2368–2375. doi: 10.1172/JCI106735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johansson B., Klein E. Cell surface localized IgM-kappa immunoglobulin reactivity in a case of chronic lymphocytic leukaemia. Clin Exp Immunol. 1970 Mar;6(3):421–428. [PMC free article] [PubMed] [Google Scholar]
  12. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968 Jul;28(7):1300–1310. [PubMed] [Google Scholar]
  13. Papamichail M., Brown J. C., Holborow E. J. Immunoglobulins on the surface of human lymphocytes. Lancet. 1971 Oct 16;2(7729):850–852. doi: 10.1016/s0140-6736(71)90224-8. [DOI] [PubMed] [Google Scholar]
  14. Pernis B., Forni L., Amante L. Immunoglobulins as cell receptors. Ann N Y Acad Sci. 1971 Dec 31;190:420–431. doi: 10.1111/j.1749-6632.1971.tb13552.x. [DOI] [PubMed] [Google Scholar]
  15. Rubin A. D., Havemann K., Dameshek W. Studies in chronic lymphocytic leukemia: further studies of the proliferative abnormality of the blood lymphocyte. Blood. 1969 Feb;33(2):313–328. [PubMed] [Google Scholar]
  16. Shevach E. M., Herberman R., Frank M. M., Green I. Receptors for complement and immunoglobulin on human leukemic cells and human lymphoblastoid cell lines. J Clin Invest. 1972 Aug;51(8):1933–1938. doi: 10.1172/JCI106999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith J. L., Cowling D. C., Barker C. R. Response of lymphocytes in chronic lymphocytic leukaemia to plant mitogens. Lancet. 1972 Jan 29;1(7744):229–233. doi: 10.1016/s0140-6736(72)90624-1. [DOI] [PubMed] [Google Scholar]
  18. Wilson J. D., Nossal G. J. Identification of human T and B lymphocytes in normal peripheral blood and in chronic lymphocytic leukaemia. Lancet. 1971 Oct 9;2(7728):788–791. doi: 10.1016/s0140-6736(71)92741-3. [DOI] [PubMed] [Google Scholar]
  19. Yoshida T. O., Imai K. Auto-antibody to human leukemic cell membrane as detected by immune adherence. Rev Eur Etud Clin Biol. 1970 Jan;15(1):61–65. [PubMed] [Google Scholar]
  20. van Furth R., Gorter H., Nadkarni J. S., Nadkarni J. J., Klein E., Clifford P. Synthesis of immunoglobulins by biopsied tissues and cell lines from Burkitt's lymphoma. Immunology. 1972 May;22(5):847–857. [PMC free article] [PubMed] [Google Scholar]

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