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. 1973 Jul;14(3):457–467.

Standardization of lymphocyte transformation to phytohaemagglutinin

M Yamamura
PMCID: PMC1553835  PMID: 4725399

Abstract

In contrast to the million cells used by many current workers, careful analysis of lymphocyte transformation to phytohaemagglutinin determined optimum conditions as (i) 200,000 lymphocytes in 1 ml of culture fluid in 100 × 12-mm round-bottomed glass tubes (ii) phytohaemagglutinin at 1 μg/tube (iii) 3H-thymidine at 1 μCi/tube (iv) with a convenient incorporation time 4 hr (v) starting 44 hr after adding phytohaemagglutinin. It was then found 10% autologous serum was the optimum concentration in the culture fluid. The log–normal range of transformation indices for young adults was 27–93–255.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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