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. 1973 May;14(1):57–67.

Membrane dynamics of HL-A–anti-HL-A complexes of human normal and leukaemic lymphocytes

H-D Menne, H-D Flad
PMCID: PMC1553852  PMID: 4123743

Abstract

Membrane dynamics of human lymphocytes treated with anti-HL-A antibodies were studied by means of indirect membrane fluorescence. When cells were incubated at 37°C for several hours, a striking diminution of the labelling index was observed. Furthermore, the pattern of the membrane fluorescence changed from a ring-shaped or patchy fluorescence to a polar concentration of the label (`cap formation'). `Cap formation' could be inhibited by incubation at 0°C and by 3 × 10−2 M sodium azide. Part of the labelled HL-A–anti-HL-A complexes could be detected within intracellular vesicles, which suggests uptake by endocytosis.

Lymphocytes of patients with chronic lymphocytic leukaemia (CLL) did not show a decrease of the labelling index. `Cap formation' was almost completely lacking, and uptake of HL-A–anti-HL-A complexes by endocytosis was not observed.

These findings indicate an impairment of membrane dynamics of CLL lymphocytes.

The importance of these membrane dynamics for the activation of lymphocytes is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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