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. 1973 Sep;15(1):93–101.

determination of IgG antiglobulins in rheumatoid disease using insolubilized human IgG

O D Ilter, M W Turner
PMCID: PMC1553870  PMID: 4203129

Abstract

IgG antiglobulins in human sera were measured by single radial diffusion in agar after elution from ethyl chloroformate insolubilized immunosorbents of rabbit or human IgG. With both immunosorbents seronegative and seropositive rheumatoid sera contained significantly higher levels of IgG antiglobulins than did sera from healthy adults. Attempts to measure antiglobulins interacting specifically with the pFc' half of the Fc region were not successful due to an unacceptably high degree of non-antibody binding.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G. N., Clark R. A., Vaughan J. H. Characterization of an IgA rheumatoid factor: finding properties and reactivity with the subclasses of human G globulin. Immunochemistry. 1972 Mar;9(3):301–315. doi: 10.1016/0019-2791(72)90094-8. [DOI] [PubMed] [Google Scholar]
  2. Anderson S. G., Bentzon M. W., Houba V., Krag P. International reference preparation of rheumatoid arthritis serum. Bull World Health Organ. 1970;42(2):311–318. [PMC free article] [PubMed] [Google Scholar]
  3. Avrameas S., Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J Biol Chem. 1967 Apr 10;242(7):1651–1659. [PubMed] [Google Scholar]
  4. Bianco N. E., Panush R. S., Stillman J. S., Schur P. H. Immunologic studies of juvenile rheumatoid arthritis. Arthritis Rheum. 1971 Nov-Dec;14(6):685–696. doi: 10.1002/art.1780140603. [DOI] [PubMed] [Google Scholar]
  5. Cerottini J. C., Grey H. M. Binding properties of monoclonal gammaG-antiglobulin factors with human gamma G. Ann N Y Acad Sci. 1969 Dec 10;168(1):76–83. doi: 10.1111/j.1749-6632.1969.tb43096.x. [DOI] [PubMed] [Google Scholar]
  6. Howell F. A., Chamberlain M. A., Perry R. A., Torrigiani G., Roitt I. M. IgG antiglobulin levels in patients with psoriatic arthropathy, ankylosing spondylitis, and gout. Ann Rheum Dis. 1972 Mar;31(2):129–131. doi: 10.1136/ard.31.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  8. Natvig J. B., Gaarder P. I., Turner M. W. IgG antigens of the C gamma 2 and C gamma 3 homology regions interacting with rheumatoid factors. Clin Exp Immunol. 1972 Oct;12(2):177–184. [PMC free article] [PubMed] [Google Scholar]
  9. Natvig J. B., Turner M. W. Rheumatoid anti Gm gactors with specificity for the pFc' subfragment of human immunoglobulin G. Nature. 1970 Feb 28;225(5235):855–857. doi: 10.1038/225855a0. [DOI] [PubMed] [Google Scholar]
  10. Normansell D. E. Anti- -globulins in rheumatoid arthritis sera. II. The reactivity of anti- -globulin rheumatoid factors with altered G-globulin. Immunochemistry. 1971 Jul;8(7):593–602. doi: 10.1016/0019-2791(71)90200-x. [DOI] [PubMed] [Google Scholar]
  11. Panush R. S., Bianco N. E., Schur P. H. Serum and synovial fluid IgG, IgA and IgM antigammaglobulins in rheumatoid arthritis. Arthritis Rheum. 1971 Nov-Dec;14(6):737–747. doi: 10.1002/art.1780140609. [DOI] [PubMed] [Google Scholar]
  12. Rowe D. S., Anderson S. G., Grab B. A research standard for human serum immunoglobulins IgG, IgA and IgM. Bull World Health Organ. 1970;42(4):535–552. [PMC free article] [PubMed] [Google Scholar]
  13. Rowe D. S., Grab B., Anderson S. G. An international reference preparation for human serum immunoglobulins G, A and M: content of immunoglobulins by weight. Bull World Health Organ. 1972;46(1):67–79. [PMC free article] [PubMed] [Google Scholar]
  14. Stone M. J., Metzger H. Binding properties of a Waldenström macroglobulin antibody. J Biol Chem. 1968 Nov 25;243(22):5977–5984. [PubMed] [Google Scholar]
  15. Torrigiani G., Ansell B. M., Chown E. E., Roitt I. M. Raised IgG antiglobulin factors in Still's disease. Ann Rheum Dis. 1969 Jul;28(4):424–427. doi: 10.1136/ard.28.4.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Torrigiani G., Roitt I. M. Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes. Ann Rheum Dis. 1967 Jul;26(4):334–340. doi: 10.1136/ard.26.4.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Torrigiani G., Roitt I. M., Lloyd K. N., Corbett M. Elevated IgG antiglobulins in patients with seronegative rheumatoid arthritis. Lancet. 1970 Jan 3;1(7636):14–16. doi: 10.1016/s0140-6736(70)90524-6. [DOI] [PubMed] [Google Scholar]
  18. Turner M. W., Bennich H. Subfragments from the Fc fragment of human immunoglobulin G. Isolation and physicochemical charaterization. Biochem J. 1968 Mar;107(2):171–178. doi: 10.1042/bj1070171. [DOI] [PMC free article] [PubMed] [Google Scholar]

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