Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
letter
. 1973 Oct;15(2):303–307.

Detection by membrane immunofluorescence of isoantibodies during rejection of skin allografts

G A Brazenor, D J Davies
PMCID: PMC1553875  PMID: 4586841

Abstract

Isoantibodies which react strongly and specifically with donor lymphocytes have been demonstrated by membrane immunofluorescence in the sera of mice and rabbits bearing first and second set allografts of skin. Changes in the titre of these antibodies closely paralleled the course of the rejection reaction. With other types of cells the immunofluorescent staining was less intense and the reactions were less consistent. Staining was completely inhibited by serum absorption with spleen, lymph node or thymus, but myocardium, aorta or tendon had no specific absorbing effect. The antibodies detected are probably directed against histocompatibility antigens.

Full text

PDF
303

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALTMAN B., SIMONSEN M. CYTOTOXIC ANTIBODY AND HEMAGGLUTININ IN CANINE HOMOTRANSPLANTATION. Ann N Y Acad Sci. 1964 Nov 30;120:28–35. doi: 10.1111/j.1749-6632.1964.tb34704.x. [DOI] [PubMed] [Google Scholar]
  2. BAILEY D. W., USAMA B. A rapid method of grafting skin on tails of mice. Transplant Bull. 1960 Apr;7:424–425. doi: 10.1097/00006534-196004000-00045. [DOI] [PubMed] [Google Scholar]
  3. BALLANTYNE D. L., Jr, STETSON C. A., Jr SEROLOGIC REACTIONS TO SKIN HOMOGRAFTS OF VARIOUS SIZES IN THE RAT. Ann N Y Acad Sci. 1964 Nov 30;120:7–14. doi: 10.1111/j.1749-6632.1964.tb34700.x. [DOI] [PubMed] [Google Scholar]
  4. Berah M., Hors J., Dausset J. A study of HL-A antigens in human organs. Transplantation. 1970 Mar;9(3):185–192. doi: 10.1097/00007890-197003000-00001. [DOI] [PubMed] [Google Scholar]
  5. Brazenor G. A., Davies D. J. A micromethod for membrane immunofluorescence. J Immunol Methods. 1973 Apr;2(3):325–328. doi: 10.1016/0022-1759(73)90059-8. [DOI] [PubMed] [Google Scholar]
  6. Cerottini J. C., Brunner K. T. Localization of mouse isoantigens on the cell surface as revealed by immunofluorescence. Immunology. 1967 Oct;13(4):395–403. [PMC free article] [PubMed] [Google Scholar]
  7. HOROWITZ R. E., BURROWS L., PARONETTO F., DREILLING D., KARK A. E. IMMUNOLOGIC OBSERVATIONS ON HOMOGRAFTS. II. THE CANINE KIDNEY. Transplantation. 1965 May;3:318–325. doi: 10.1097/00007890-196505000-00002. [DOI] [PubMed] [Google Scholar]
  8. McKenzie I. F., Whittingham S. Deposits of immunoglobulin and fibrin in human allografted kidneys. Lancet. 1968 Dec 21;2(7582):1313–1316. doi: 10.1016/s0140-6736(68)91815-1. [DOI] [PubMed] [Google Scholar]
  9. Porter K. A., Andres G. A., Calder M. W., Dossetor J. B., Hsu K. C., Rendall J. M., Seegal B. C., Starzl T. E. Human renal transplants. II. Immunofluorescent and immunoferritin studies. Lab Invest. 1968 Feb;18(2):159–171. [PubMed] [Google Scholar]
  10. Rolland J. M., Nairn R. C., Davies D. J. Assay of anti-lymphocyte serum by membrane immunofluorescence. J Immunol Methods. 1971 Sep;1(1):83–90. doi: 10.1016/0022-1759(71)90007-x. [DOI] [PubMed] [Google Scholar]
  11. Rossen R. D., Butler W. T., Reisberg M. A., Brooks D. K., Leachman R. D., Milam J. D., Mittal K. K., Montgomery J. R., Nora J. J., Rochelle D. G. Immunofluorescent localization of human immunoglobulin in tissues from cardiac allograft recipients. J Immunol. 1971 Jan;106(1):171–180. [PubMed] [Google Scholar]
  12. YOUNG R. M., HOPKINS J. P., ESPARZA A. HUMORAL ASPECTS OF SKIN TRANSPLANTATION IN THE RABBIT. Am J Pathol. 1963 Aug;43:227–245. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES