Abstract
The effect of an isolated calf foetal protein, foetuin, on lymphocyte cultures in vitro was studied using bovine serum albumin and lyophilized calf serum as controls. Four different lots of foetuin were found to induce blast transformation and protein and DNA synthesis of human peripheral blood lymphocytes. The peak responses were reached after 5–6 days of culture. Lymphocytes from bovine mesenteric lymph-nodes were also stimulated after 6 days of culture. The lymphocyte stimulating property associated with foetuin was stable to freeze-thawing but destroyed by heating at 80°C for 60 min. HeLa cell growth promoting factor known to be present in ammonium sulphate-precipitated foetuin preparations was excluded as being responsible for the mitogenic effect observed.
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Selected References
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- BERGMANN F. H., LEVINE L., SPIRO R. G. Fetuin: immunochemistry and quantitative estimation in serum. Biochim Biophys Acta. 1962 Mar 26;58:41–51. doi: 10.1016/0006-3002(62)90815-6. [DOI] [PubMed] [Google Scholar]
- Bloch-Shtacher N., Hirschhorn K., Uhr J. W. The response of lymphocytes from non-immunized humans to antigen-antibody complexes. Clin Exp Immunol. 1968 Nov;3(9):889–899. [PMC free article] [PubMed] [Google Scholar]
- Fisher H. W., Puck T. T., Sato G. MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS: THE ACTION OF FETUIN IN PROMOTING CELL ATTACHMENT TO GLASS. Proc Natl Acad Sci U S A. 1958 Jan;44(1):4–10. doi: 10.1073/pnas.44.1.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heilman D. H. Mitogenic activity of bacterial fractions in lymphocyte cultures. Endotoxins and other derivatives of gram-negative bacteria. Int Arch Allergy Appl Immunol. 1970;39(4):415–425. doi: 10.1159/000230369. [DOI] [PubMed] [Google Scholar]
- Hsu C. C., Leevy C. M. Inhibition of PHA-stimulated lymphocyte transformation by plasma from patients with advanced alcoholic cirrhosis. Clin Exp Immunol. 1971 May;8(5):749–760. [PMC free article] [PubMed] [Google Scholar]
- Johnson G. J., Russell P. S. Reaction of human lymphocytes in culture to components of the medium. Nature. 1965 Oct 23;208(5008):343–345. doi: 10.1038/208343a0. [DOI] [PubMed] [Google Scholar]
- Kornfeld S., Kornfeld R. Solubilization and partial characterization of a phytohemagglutinin receptor site from human erythrocytes. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1439–1446. doi: 10.1073/pnas.63.4.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIEBERMAN I., LAMY F., OVE P. Nonidentity of fetuin and protein growth (flattening) factor. Science. 1959 Jan 2;129(3340):43–44. doi: 10.1126/science.129.3340.43. [DOI] [PubMed] [Google Scholar]
- Möller G. Induction of DNA synthesis in normal human lymphocyte cultures by antigen-antibody complexes. Clin Exp Immunol. 1969 Jan;4(1):65–82. [PMC free article] [PubMed] [Google Scholar]
- Peavy D. L., Adler W. H., Smith R. T. The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. J Immunol. 1970 Dec;105(6):1453–1458. [PubMed] [Google Scholar]
- Puck T. T., Waldren C. A., Jones C. Mammalian cell growth proteins. I. Growth stimulation of fetuin. Proc Natl Acad Sci U S A. 1968 Jan;59(1):192–199. doi: 10.1073/pnas.59.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spiro R. G. Studies on fetuin, a glycoprotein of fetal serum. I. Isolation, chemical composition, and physiochemical properties. J Biol Chem. 1960 Oct;235(10):2860–2869. [PubMed] [Google Scholar]
- Vogt A., Mishell R. I., Dutton R. W. Stimulation of DNA synthesis in cultures of mouse spleen cell suspensions by bovine transferrin. Exp Cell Res. 1969 Feb;54(2):195–200. doi: 10.1016/0014-4827(69)90232-8. [DOI] [PubMed] [Google Scholar]
- Woodliff H. J., Onesti P. Blastoid transformation of lymphocytes in vitro: the stimulatory effect of foetal calf serum. Med J Aust. 1968 Jun 22;1(25):1089–1091. doi: 10.5694/j.1326-5377.1968.tb29166.x. [DOI] [PubMed] [Google Scholar]