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. 1974 Feb;16(2):213–222.

Passive sensitization of guinea-pig skin in vitro for the antigen-induced release of anaphylactic mediators

D G Jones, A B Kay
PMCID: PMC1553927  PMID: 4143274

Abstract

Guinea-pig skin fragments were passively sensitized for the antigen-induced release of histamine, SRS-A and ECF-A. Skin histamine was chemically identified by fluorimetry; SRS-A gave a characteristic dose-dependent contraction of the guinea-pig ileum; and ECF-A selectively attracted eosinophils. The antibody mediating the release of these agents was shown to be IgG1. Following antigen challenge different time courses of release were demonstrable for histamine, SRS-A and ECF-A. Skin SRS-A was resistant to treatment with the enzyme pronase and skin ECF-A had an estimated molecular size of between 500 and 1000. They were therefore comparable to similar agents released from the lung. Thus SRS-A and ECF-A join histamine as chemical mediators in cutaneous anaphylaxis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER A. R., BLOCH K. J., AUSTEN K. F. IN VITRO PASSIVE SENSITIZATION OF CHOPPED GUINEA PIG LUNG BY GUINEA PIG 7S ANTIBODIES. J Immunol. 1964 Oct;93:525–531. [PubMed] [Google Scholar]
  2. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BROCKLEHURST W. E. Slow reacting substance and related compounds. Prog Allergy. 1962;6:539–558. [PubMed] [Google Scholar]
  4. BROCKLEHURST W. E. The release of histamine and formation of a slow-reacting substance (SRS-A) during anaphylactic shock. J Physiol. 1960 Jun;151:416–435. doi: 10.1113/jphysiol.1960.sp006449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dvorak H. F., Dvorak A. M., Simpson B. A., Richerson H. B., Leskowitz S., Karnovsky M. J. Cutaneous basophil hypersensitivity. II. A light and electron microscopic description. J Exp Med. 1970 Sep 1;132(3):558–582. doi: 10.1084/jem.132.3.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FISHER J. P., COOKE R. A. Passive cutaneous anaphylaxis (PCA) in the guinea pig; an immunologic and pathologic study. J Allergy. 1957 Mar;28(2):150–169. doi: 10.1016/0021-8707(57)90021-7. [DOI] [PubMed] [Google Scholar]
  7. Greaves M. W., Fairley V. M., Yamamoto S. Release of histamine from skin during in vitro anaphylaxis. Cutaneous anaphylaxis in vitro. Int Arch Allergy Appl Immunol. 1971;41(6):932–939. doi: 10.1159/000230584. [DOI] [PubMed] [Google Scholar]
  8. Greaves M. W., Yamamoto S., Fairley V. M. IgE-mediated hypersensitivity in human skin studied using a new in vitro method. Immunology. 1972 Aug;23(2):239–248. [PMC free article] [PubMed] [Google Scholar]
  9. Kay A. B., Shin H. S., Austen K. F. Selective attraction of eosinophils and synergism between eosinophil chemotactic factor of anaphylaxis (ECF-A) and a fragment cleaved from the fifth component of complement (C5a). Immunology. 1973 Jun;24(6):969–976. [PMC free article] [PubMed] [Google Scholar]
  10. Kay A. B., Stechschulte D. J., Austen K. F. An eosinophil leukocyte chemotactic factor of anaphylaxis. J Exp Med. 1971 Mar 1;133(3):602–619. doi: 10.1084/jem.133.3.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kay A. B. Studies on eosinophil leucocyte migration. I. Eosinophil and neutrophil accumulation following antigen-antibody reactions in guinea-pig skin. Clin Exp Immunol. 1970 Jan;6(1):75–86. [PMC free article] [PubMed] [Google Scholar]
  12. MONGAR J. L., SCHILD H. O. A comparison of the effects of anaphylactic shock and of chemical histamine releasers. J Physiol. 1952 Dec;118(4):461–478. doi: 10.1113/jphysiol.1952.sp004808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OATES J. A., MARSH E., SJOERDSMA A. Studies on histamine in human urine using a fluorometric method of assay. Clin Chim Acta. 1962 Jul;7:488–497. doi: 10.1016/0009-8981(62)90089-x. [DOI] [PubMed] [Google Scholar]
  14. Stechschulte D. J., Austen K. F., Bloch K. J. Antibodies involved in antigen-induced release of slow reacting substance of anaphylaxis (SRS-A) in the guinea pig and rat. J Exp Med. 1967 Jan 1;125(1):127–147. doi: 10.1084/jem.125.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yeoh T. S., Tay C. H., Greaves M. W. Anaphylactic release of histamine from guinea pig skin in vitro. Int Arch Allergy Appl Immunol. 1972;42(3):485–490. doi: 10.1159/000230628. [DOI] [PubMed] [Google Scholar]

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