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. 1974 Mar;16(3):445–452.

Metabolism of radio-labelled C3: effects of in vivo activation in rabbits

J A Charlesworth, D G Williams, P Naish, P J Lachmann, D K Peters
PMCID: PMC1553954  PMID: 4468849

Abstract

Turnover studies were performed in rabbits using biologically screened, highly purified, radio-labelled human C3 and C3c. Experiments were also carried out using agents known to activate the complement system in vivo—cobra venom factor, human nephritic serum and nephrotoxic antibody to rabbit glomerular basement membrane. Activation of labelled C3 by cobra factor provided information regarding the metabolic behaviour of C3d.

The fractional catabolic ratio (FCR) of human C3 in normal rabbits was 2·4–2·8% of the plasma pool per hr. FCR for C3c was 4.5% per hr. Activation of C3 in vivo consistently resulted in accelerated disappearance of plasma radioactivity. Analysis of the plasma and total body radioactivity curves indicated that both hypercatabolism and extravascular sequestration of radioactivity were responsible for this phenomenon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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