Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1973 Dec;15(4):557–563.

Antigen binding and complement fixing activity of IgM molecules reassociated in the presence and absence of J chains

E Kownatzki, M Drescher
PMCID: PMC1553969  PMID: 4205038

Abstract

Monoclonal IgM with anti-Pr2 activity was purified from the serum of a patient with chronic cold agglutinin disease. After reduction with 20 mM dithiothreitol IgM molecules and J chains were separated. The subunits were reoxidized in the presence and in the absence of J chains. In both mixtures cold agglutinin activity against the Pr2 antigen was restored to the titre of the native IgM. Complement fixation as measured by the C1 fixation and transfer test was recovered in the reassociated materials no matter whether J chains were present or not. It was concluded that the presence of J chains was not required for antigen binding and complement fixing activity of IgM antibodies.

Full text

PDF
557

Images in this article

559FIG. 1
on p.559

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beale D., Feinstein A. Studies on the reduction of a human 19S immunoglobulin M. Biochem J. 1969 Apr;112(2):187–194. doi: 10.1042/bj1120187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borsos T., Rapp H. J. Hemolysin titration based on fixation of the activated first component of complement: evidence that one molecule of hemolysin suffices to sensitize an erythrocyte. J Immunol. 1965 Sep;95(3):559–566. [PubMed] [Google Scholar]
  3. Borsos T., Rapp H. J. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement. J Immunol. 1967 Aug;99(2):263–268. [PubMed] [Google Scholar]
  4. HARBOE M., DEVERILL J. IMMUNOCHEMICAL PROPERTIES OF COLD HAEMAGGLUTININS. Scand J Haematol. 1964;1:223–237. doi: 10.1111/j.1600-0609.1964.tb00019.x. [DOI] [PubMed] [Google Scholar]
  5. Halpern M. S., Coffman R. L. Polymer formation and J chain synthesis in mouse plasmacytomas. J Immunol. 1972 Oct;109(4):674–680. [PubMed] [Google Scholar]
  6. Halpern M. S., Koshland M. E. Noval subunit in secretory IgA. Nature. 1970 Dec 26;228(5278):1276–1278. doi: 10.1038/2281276a0. [DOI] [PubMed] [Google Scholar]
  7. Harboe M., Solheim B. G., Deverill J. Individual antigenic specificity of human macroglobulins: Localization of antigenic determinants and their use to demonstrate formation of hybrid molecules after reduction and reassociation. J Exp Med. 1969 Jun 1;129(6):1217–1234. doi: 10.1084/jem.129.6.1217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jerry L. M., Kunkel H. G., Adams L. Stabilization of dissociable IgA2 proteins by secretory component. J Immunol. 1972 Aug;109(2):275–283. [PubMed] [Google Scholar]
  9. Kownatzki E. Reassociation of IgM subunits in the presence and absence of J chain. Immunol Commun. 1973;2(1):105–113. doi: 10.3109/08820137309022886. [DOI] [PubMed] [Google Scholar]
  10. Kownatzki E. Studies on a fragment of human IgM macroglobulins related to "J chain". Eur J Immunol. 1971 Dec;1(6):486–491. doi: 10.1002/eji.1830010616. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. MULLER-EBERHARD H. J. A new supporting medium for preparative electrophoresis. Scand J Clin Lab Invest. 1960;12:33–37. [PubMed] [Google Scholar]
  13. MURRAY E. S., O'CONNOR J. M., GAON J. A. DIFFERENTIATION OF 19S AND 7S COMPLEMENT FIXING ANTIBODIES IN PRIMARY VERSUS RECRUDESCENT TYPHUS BY EITHER ETHANETHIOL OR HEAT. Proc Soc Exp Biol Med. 1965 May;119:291–297. doi: 10.3181/00379727-119-30161. [DOI] [PubMed] [Google Scholar]
  14. Mestecky J., Zikan J., Butler W. T. Immunoglobulin M and secretory immunoglobulin A: presence of a common polypeptide chain different from light chains. Science. 1971 Mar 19;171(3976):1163–1165. doi: 10.1126/science.171.3976.1163. [DOI] [PubMed] [Google Scholar]
  15. Plaut A. G., Cohen S., Tomasi T. B., Jr Immunoglobulin M: fixation of human complement by the Fc fragment. Science. 1972 Apr 7;176(4030):55–56. doi: 10.1126/science.176.4030.55. [DOI] [PubMed] [Google Scholar]
  16. Roelcke D. Besonderheiten der Reaktionsweise eines mit Plasmocytom-gammaA-Paraprotein identischen Kälteagglutinins. Klin Wochenschr. 1968 Feb 1;46(3):126–131. doi: 10.1007/BF01727370. [DOI] [PubMed] [Google Scholar]
  17. Roelcke D., Jungfer H. Kombinierte mu-gamma-Antigenität von Paraprotein-Immunglobulinem mit Kälteautoantikörper-Aktivität. Klin Wochenschr. 1970 Aug 1;48(15):914–918. doi: 10.1007/BF01487630. [DOI] [PubMed] [Google Scholar]
  18. Roelcke D., Uhlenbruck G. Antigen nomenclature. Vox Sang. 1970 May;18(5):478–479. doi: 10.1111/j.1423-0410.1970.tb01488.x. [DOI] [PubMed] [Google Scholar]
  19. Roelcke D., Uhlenbruck G., Bauer K. A heterogeneity of the HD-receptor, demonstrable by HD-cold antibodies: HD1-HD2-immunochemical aspects. Scand J Haematol. 1969;6(4):280–287. [PubMed] [Google Scholar]
  20. Stollar B. D., Sandberg A. L. Comparisons of antibodies reacting with DNA. I. Systemic lupus erythematosus sera and rabbit antibodies induced by DNA-methylated bovine serum albumin complexes. J Immunol. 1966 May;96(5):755–763. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES