Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1973 Dec;15(4):549–556.

Quantitative determination of hepatitis B antigen by means of radial immunodiffusion

K Madaliński, W J Brzosko, Agata Budkowska, Barbara Mikulska
PMCID: PMC1553971  PMID: 4205037

Abstract

The method of radial immunodiffusion for measurement of hepatitis B antigen (HB ag) levels in sera of twenty and urines of three HB ag positive patients is described. In patients suffering from acute hepatitis, the level of HB ag in serum was 0·15–2·12 mg/ml (mean = 1·08±SD 0·69 mg/ml). In patients suffering from chronic hepatitis the level of HB ag in serum was 0·36–3·61 mg/ml (mean 2·56±SD 1·13 mg/ml). The highest levels of HB ag in serum, 2·62–5·12 mg/ml (mean 3·91±SD 0·90 mg/ml) were observed in symptomless HB ag carriers.

The amounts of HB ag excreted in urine, found in two chronic hepatitis patients and one HB ag carrier, were 13·2 mg, 46 mg and 82 mg/1000 ml of urine respectively.

Full text

PDF
549

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D., Waterson A. P. Immune complexes in hepatitis. Lancet. 1969 Nov 8;2(7628):983–986. doi: 10.1016/s0140-6736(69)90540-6. [DOI] [PubMed] [Google Scholar]
  2. Bayer M. E., Blumberg B. S., Werner B. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis. Nature. 1968 Jun 15;218(5146):1057–1059. doi: 10.1038/2181057a0. [DOI] [PubMed] [Google Scholar]
  3. Berg R., Ringertz O., Espmark A. Australia antigen in hepatitis among Swedish track-finders. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):423–427. doi: 10.1111/j.1699-0463.1971.tb00083.x. [DOI] [PubMed] [Google Scholar]
  4. Boenisch T., Katz G. Hepatitis-associated antigen (HAA). 1. Quantitation by electrophoresis in antibody-containing agarose gel by use of a primary standard of purified HAA. Clin Chem. 1971 Jul;17(7):595–601. [PubMed] [Google Scholar]
  5. Brzosko W. J., Madalinski K., Krawczynski K., Skwarska H., Nowoslawski A. Australia antigen immune complexes in patients with different forms of hepatitis. J Infect Dis. 1971 Mar;123(3):251–256. doi: 10.1093/infdis/123.3.251. [DOI] [PubMed] [Google Scholar]
  6. Dane D. S., Cameron C. H., Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970 Apr 4;1(7649):695–698. doi: 10.1016/s0140-6736(70)90926-8. [DOI] [PubMed] [Google Scholar]
  7. Davidson C. Potassium supplements and diuretics. Lancet. 1972 Oct 21;2(7782):882–882. doi: 10.1016/s0140-6736(72)92254-4. [DOI] [PubMed] [Google Scholar]
  8. Dudley F. J., Fox R. A., Sherlock S. Relationship of hepatitis-associated antigen (H.A.A.) to acute and chronic liver injury. Lancet. 1971 Jul 3;2(7714):1–3. doi: 10.1016/s0140-6736(71)90001-8. [DOI] [PubMed] [Google Scholar]
  9. FAHEY J. L., MCKELVEY E. M. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed] [Google Scholar]
  10. Grob P. J., Jemelka H. Faecal S.H. (Australia) antigen in acute hepatitis. Lancet. 1971 Jan 30;1(7692):206–208. doi: 10.1016/s0140-6736(71)90949-4. [DOI] [PubMed] [Google Scholar]
  11. Kim C. Y. Hepatitis-associated antigen. Its biophysical properties and nucleic acid. Vox Sang. 1971 May;20(5):461–462. doi: 10.1111/j.1423-0410.1971.tb01823.x. [DOI] [PubMed] [Google Scholar]
  12. Kim C. Y., Tilles J. G. Quantitation of hepatitis-associated antigen with a modified Ouchterlony precipitin test. J Infect Dis. 1971 Nov;124(5):512–516. doi: 10.1093/infdis/124.5.512. [DOI] [PubMed] [Google Scholar]
  13. Krawczyński K., Nazarewicz T., Brzosko W. J., Nowoslawski A. Cellular localization of hepatitis-associated antigen in livers of patients with different forms of hepatitis. J Infect Dis. 1972 Oct;126(4):372–377. doi: 10.1093/infdis/126.4.372. [DOI] [PubMed] [Google Scholar]
  14. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  15. Le Bouvier G. L. The heterogeneity of Australia antigen. J Infect Dis. 1971 Jun;123(6):671–675. doi: 10.1093/infdis/123.6.671. [DOI] [PubMed] [Google Scholar]
  16. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  17. Nagington J. Chronic carriage of Australia antigen without symptoms after renal transplant. Br Med J. 1970 Nov 14;4(5732):409–410. doi: 10.1136/bmj.4.5732.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Prince A. M. An antigen detected in the blood during the incubation period of serum hepatitis. Proc Natl Acad Sci U S A. 1968 Jul;60(3):814–821. doi: 10.1073/pnas.60.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shulman N. R., Barker L. F. Virus-like antigen, antibody, and antigen-antibody complexes in hepatitis measured by complement fixation. Science. 1969 Jul 18;165(3890):304–306. doi: 10.1126/science.165.3890.304. [DOI] [PubMed] [Google Scholar]
  20. Skinhöj P., Thulstrup H. Hepatitis-associated antigen. Qualitative and quantitative determination. Scand J Clin Lab Invest. 1971 Feb;27(1):61–66. doi: 10.3109/00365517109080190. [DOI] [PubMed] [Google Scholar]
  21. Tripatzis I., Horst H. G. Detection of Australia-SH-antigen in urine. Nature. 1971 May 28;231(5300):266–267. doi: 10.1038/231266a0. [DOI] [PubMed] [Google Scholar]
  22. Vyas G. N., Shulman N. R. Hemagglutination assay for antigen and antibody associated with viral hepatitis. Science. 1970 Oct 16;170(3955):332–333. doi: 10.1126/science.170.3955.332. [DOI] [PubMed] [Google Scholar]
  23. Walsh J. H., Yalow R., Berson S. A. Detection of Australia antigen and antibody by means of radioimmunoassay techniques. J Infect Dis. 1970 May;121(5):550–554. doi: 10.1093/infdis/121.5.550. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES