Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1974 May;17(1):189–198.

In vitro spleen leucocyte migration and phytohaemagglutinin-stimulated lymphocyte transformation of immunosuppressed mice cells. II. Cells from mice treated by azathioprine and/or immune stimulation or thymectomy

A K House, J L Boak, Violet Draper
PMCID: PMC1554053  PMID: 4549569

Abstract

An in vitro study of the spleen leucocyte migration ability and the phytohaemag-glutinin (PHA) stimulated peripheral lymphocyte response was made on cells derived from mice treated by azathioprine, immune stimulation, or thymectomy and azathioprine. These responses were compared with those shown by normal mice and found to be considerably depressed. Thymectomy combined with azathioprine produced the greatest change which persisted at 40 days. These changes suggested that azathioprine in common with thymectomy, antilymphocyte globulin and radiotherapy produced some of its immunosuppression by depressing T-cell function.

Full text

PDF
189

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach J. F., Muller J. Y., Dardenne M. In vivo specific antigen recognition by rosette forming cells. Nature. 1970 Sep 19;227(5264):1251–1252. doi: 10.1038/2271251a0. [DOI] [PubMed] [Google Scholar]
  2. Bach M. A., Bach J. F. Activities of immunosuppressive agents in vitro. II. Different timing of azathioprine and methotrexate in inhibition and stimulation of mixed lymphocyte reaction. Clin Exp Immunol. 1972 May;11(1):89–98. [PMC free article] [PubMed] [Google Scholar]
  3. Doenhoff M. J. The effect of x-rays on circulating lymphocytes of mice quantitated by PHA-responsiveness. Clin Exp Immunol. 1971 Apr;8(4):603–615. [PMC free article] [PubMed] [Google Scholar]
  4. ELION G. B., BIEBER S., HITCHINGS G. H. The fate of 6-mercaptopurine in mice. Ann N Y Acad Sci. 1954 Dec 6;60(2):297–303. doi: 10.1111/j.1749-6632.1954.tb40020.x. [DOI] [PubMed] [Google Scholar]
  5. Heine K. M., Stobbe H., Klatt R., Apostoloff E., Dutz W. Lymphocyte transformation tests in patients under treatment with immunosuppressive drugs. Helv Med Acta. 1969 Nov;35(2):140–145. [PubMed] [Google Scholar]
  6. House A. K., Boak J. L., Draper V. In vitro spleen leucocyte migration and phytohaemagglutinin-stimulated lymphocyte transformation of immunosuppressed mice cells. I. Cells from mice treated by thymectomy and/or rabbit anti-mouse lymphocyte serum. Clin Exp Immunol. 1974 May;17(1):179–187. [PMC free article] [PubMed] [Google Scholar]
  7. Jenkins J. D., Stahl W. M. Lymphocyte trapping in dog popliteal lymph nodes: the influence of acute uremia and pharmacologic immunosuppression. Ann Surg. 1968 Jan;167(1):63–72. doi: 10.1097/00000658-196801000-00009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. V. Use of antilymphocyte serum to deplete animals of helper cells. Eur J Immunol. 1971 Apr;1(2):68–75. doi: 10.1002/eji.1830010204. [DOI] [PubMed] [Google Scholar]
  9. SCHWARTZ R. S., ANDRE J. Clearance of proteins from blood of normal and 6-mercaptopurine treated rabbits. Proc Soc Exp Biol Med. 1960 Jun;104:228–230. doi: 10.3181/00379727-104-25788. [DOI] [PubMed] [Google Scholar]
  10. Schwartz G. H., Stenzel K. H., Rubin A. L. Depression of lymphocyte transformation by drugs in vitro. Effects of a new immunosuppressant, Cinanserin. Transplantation. 1969 Nov;8(5):704–711. doi: 10.1097/00007890-196911000-00015. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES