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. 1974 May;17(1):151–158.

Differentiation of human bone marrow cells into T lymphocytes by in vitro incubation with thymic extracts

J L Touraine, G S Incefy, F Touraine, Y M Rho, R A Good
PMCID: PMC1554066  PMID: 4549567

Abstract

Extracts of human or calf thymus influence differentiation of human bone marrow cells in vitro. Incubation of a putative stem cell fraction of human marrow with extracts of thymus for a period of 2 hr led to the appearance of lymphocytes with surface antigens recognized by a highly specific anti-T-cell antiserum. To a lesser degree, development of lymphocytes having the capacity to form rosettes with sheep erythrocytes was observed. Treatment of the so-called stem cell fraction with thymic extracts did not yield cells responsive to mitogenic influences of phyto-haemagglutinin or allogeneic cells. This model should permit further analyses of immunodeficiency diseases and provide a useful technique for purification and analysis of thymus extracts active with human haematopoietic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bashir H., Boyle W., MacQueen M., Tiilikainen A. A simple micro cytotoxicity test. Transplantation. 1969 Mar;7(3):220–223. doi: 10.1097/00007890-196903000-00023. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F., Dardenne M., Goldstein A. L., Guha A., White A. Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2734–2738. doi: 10.1073/pnas.68.11.2734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dicke K. A., Lina P. H., van Bekkum D. W. Adaptation of albumin density gradient centrifugation to human bone marrow fractionation. Rev Eur Etud Clin Biol. 1970 Mar;15(3):305–309. [PubMed] [Google Scholar]
  4. Goldstein A. L., Guha A., Zatz M. M., Hardy M. A., White A. Purification and biological activity of thymosin, a hormone of the thymus gland. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1800–1803. doi: 10.1073/pnas.69.7.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KLEIN E., KLEIN G. ANTIGENIC PROPERTIES OF LYMPHOMAS INDUCED BY THE MOLONEY AGENT. J Natl Cancer Inst. 1964 Mar;32:547–568. [PubMed] [Google Scholar]
  7. Komuro K., Boyse E. A. In-vitro demonstration of thymic hormone in the mouse by conversion of precursor cells into lymphocytes. Lancet. 1973 Apr 7;1(7806):740–743. doi: 10.1016/s0140-6736(73)92127-2. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. MARTINEZ C., KERSEY J., PAPERMASTER B. W., GOOD R. A. Skin homograft survival in thymectomized mice. Proc Soc Exp Biol Med. 1962 Jan;109:193–196. doi: 10.3181/00379727-109-27149. [DOI] [PubMed] [Google Scholar]
  10. MILLER J. F. Immunological function of the thymus. Lancet. 1961 Sep 30;2(7205):748–749. doi: 10.1016/s0140-6736(61)90693-6. [DOI] [PubMed] [Google Scholar]
  11. OSOBA D., MILLER J. F. THE LYMPHOD TISSUES AND IMMUNE RESPONSES OF NEONATALLY THYMECTOMIZED MICE BEARING THYMUS TISSUE IN MILLIPORE DIFFUSION CHAMBERS. J Exp Med. 1964 Jan 1;119:177–194. doi: 10.1084/jem.119.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scheid M. P., Hoffmann M. K., Komuro K., Hämmerling U., Abbott J., Boyse E. A., Cohen G. H., Hooper J. A., Schulof R. S., Goldstein A. L. Differentiation of T cells induced by preparations from thymus and by nonthymic agents. J Exp Med. 1973 Oct 1;138(4):1027–1032. doi: 10.1084/jem.138.4.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wilson O. H., Bhaumick B. Les facteurs thymiques qui induisent l'antigène theta sur les cellules de moelle osseuse. Union Med Can. 1973 Apr;102(4):834–845. [PubMed] [Google Scholar]
  14. Wybran J., Carr M. C., Fudenberg H. H. The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest. 1972 Oct;51(10):2537–2543. doi: 10.1172/JCI107069. [DOI] [PMC free article] [PubMed] [Google Scholar]

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