Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1974 Jul;17(3):463–473.

Inhibition of in vitro lymphocyte blastogenesis by inhibitor produced by cultured human lymphoblasts

E M Hersh, K B McCredie, E J Freireich
PMCID: PMC1554085  PMID: 4283280

Abstract

Normal human lymphoblastoid cell lines, growing in continuous suspension culture, produce inhibitors of in vitro lymphocyte blastogenesis. The inhibitor reduces human lymphocyte blastogenic responses to phytohaemagglutinin, streptolysin-O and the mixed lymphocyte culture 90–99%, is non-cytotoxic and can inhibit both newly initiated and on-going responses. The inhibitor is heat-stable at 80°C but labile at 100°C, non-dialysable and degraded by pronase but not DNase or RNase. It is species- and tissue-specific and does not inhibit the proliferation of mouse lymphocytes, human melanoma cells or human bone marrow in vitro colony-forming cells. Inhibitor was produced only under very specific conditions of crowding. Thus, maximal inhibitor production occurred at 5 × 106 lymphocytes per cm2 culture surface area while only 0–5% of the maximal amount was produced at 106 or 5 × 107 lymphoblasts per cm2. This data is relevant to the nature of feedback control of immunological reactions and may guide the development of new classes of immunosuppressants.

Full text

PDF
463

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dumonde D. C., Wolstencroft R. A., Panayi G. S., Matthew M., Morley J., Howson W. T. "Lymphokines": non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature. 1969 Oct 4;224(5214):38–42. doi: 10.1038/224038a0. [DOI] [PubMed] [Google Scholar]
  2. Freireich E. J., Judson G., Levin R. H. Separation and collection of leukocytes. Cancer Res. 1965 Oct;25(9):1516–1520. [PubMed] [Google Scholar]
  3. Glade P. R., Hirschhorn K. Products of lymphoid cells in continuous culture. Am J Pathol. 1970 Sep;60(3):483–494. [PMC free article] [PubMed] [Google Scholar]
  4. Green J. A., Green J. R., Cooperband S. R., Kibrick S. In vitro sensitivity of human cells to lymphocyte proliferation inhibitory factor. J Natl Cancer Inst. 1972 Sep;49(3):631–638. [PubMed] [Google Scholar]
  5. Hersh E. M., Drewinko B. Specific inhibition of lymphocyte blastogenic responses to mitogens by a factor produced by cultured human malignant lymphoma cells. Cancer Res. 1974 Jan;34(1):215–220. [PubMed] [Google Scholar]
  6. Hersh E. M., Harris J. E., Rogers E. A. Influence of cell density on response of leukocytes and column-purified lymphocytes to mitogenic agents. J Reticuloendothel Soc. 1970 May;7(5):567–586. [PubMed] [Google Scholar]
  7. Houck J. C., Irausquin H., Leikin S. Lymphocyte DNA synthesis inhibition. Science. 1971 Sep 17;173(4002):1139–1141. doi: 10.1126/science.173.4002.1139. [DOI] [PubMed] [Google Scholar]
  8. Juhasz S. E. Reciprocal in toto conversion of Mycobacterium phlei--Mycobacterium smegmatis by mediation of an intermediate hybrid genome: B2h. Nature. 1967 Apr 29;214(5087):518–520. doi: 10.1038/214518a0. [DOI] [PubMed] [Google Scholar]
  9. Kiger N., Florentin I., Mathé G. Some effects of a partially purified lymphocyte-inhibiting factor from calf thymus. Transplantation. 1972 Oct;14(4):448–454. doi: 10.1097/00007890-197210000-00007. [DOI] [PubMed] [Google Scholar]
  10. Lasalvia E., Garcia-Giralt E., Macieira-Coelho A. Extraction of an inhibitor of DNA synthesis from human peripheral blood lymphocytes and bovine spleen. Rev Eur Etud Clin Biol. 1970 Aug-Sep;15(7):789–792. [PubMed] [Google Scholar]
  11. Moorhead J. F., Paraskova-Tchernozenska E., Pirrie A. J., Hayes C. Lymphoid inhibitor of human lymphocyte DNA synthesis and mitosis in vitro. Nature. 1969 Dec 20;224(5225):1207–1208. doi: 10.1038/2241207a0. [DOI] [PubMed] [Google Scholar]
  12. Morales V. H., Garcia-Giralt E. Immunosuppressive action in vitro by a bovine spleen extract. Z Naturforsch B. 1971 Dec;26(12):1339–1340. [PubMed] [Google Scholar]
  13. Pegrum G. D., Barry C. J. The effects of adding reticulo-endothelial tissue extracts to human leucocyte cultures. J Pathol Bacteriol. 1968 Jul;96(1):187–201. doi: 10.1002/path.1700960120. [DOI] [PubMed] [Google Scholar]
  14. Rytöma T., Kiviniemi K. Control of cell production in rat chloroleukemia by means of the granulocytic chalone. Nature. 1968 Oct 12;220(5163):136–137. doi: 10.1038/220136a0. [DOI] [PubMed] [Google Scholar]
  15. Rytömaa T., Kiviniemi K. Chloroma regression induced by the granulocytic chalone. Nature. 1969 Jun 7;222(5197):995–996. doi: 10.1038/222995a0. [DOI] [PubMed] [Google Scholar]
  16. Smith R. T., Bausher J. A., Adler W. H. Studies of an inhibitor of DNA synthesis and a nonspecific mitogen elaborated by human lymphoblasts. Am J Pathol. 1970 Sep;60(3):495–504. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES