Abstract
To examine the immunomodulatory effects of HTLV infection, lymphocyte subset analysis was performed on patients infected with human T cell lymphotropic virus type-I (HTLV-I, n = 6) or -II (HTLV-II, n = 12) and on normal blood donors (n = 16). The percentages of total B lymphocytes (CD19), natural killer (NK) cells (CD16), T lymphocytes and their subsets (CD2, CD3, CD4, CD5, CD7, CD8), and IL-2R (CD25) were found to be within the range found in normal donors. However, the expression of CD8+ HLA-DR+ increased significantly in patients with HTLV-I or HTLV-II infection (14.1 +/- 3.9% and 9.7 +/- 2.4% respectively; P less than 0.01) when compared with controls (3.2 +/- 1.1%). In addition, there was a significantly greater proportion of CD4+CD29+ T lymphocytes (29.3 +/- 6.1% and 31.1 +/- 9.0%; P less than 0.05) with concomitant diminution of CD4+CD45RA+ T lymphocytes (8.3 +/- 3.3% and 11.4 +/- 1.5%; P less than 0.01) in patients infected with HTLV-I or HTLV-II respectively, when compared with controls. The increased percentage of CD4+CD29+ subpopulations showed a direct correlation (rs = 0.86; P less than 0.001) with HTLV-specific antibody production. No difference in the CD8 population coexpressing CD29 and S6F1 (an epitope of LFA-1) were observed in the HTLV-infected group when compared with normal donors and functional analysis exhibited minimal cytotoxicity against lectin labelled heterologous target cells. Thus, the shift in the suppressor/cytotoxic to helper/inducer 'memory' CD4+ may be associated with immunoregulatory abnormalities often found in persons infected with HTLV-I or HTLV-II.
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- Akbar A. N., Terry L., Timms A., Beverley P. C., Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1;140(7):2171–2178. [PubMed] [Google Scholar]
- Bell E. B., Sparshott S. M. Interconversion of CD45R subsets of CD4 T cells in vivo. Nature. 1990 Nov 8;348(6297):163–166. doi: 10.1038/348163a0. [DOI] [PubMed] [Google Scholar]
- Blattner W. A., Kalyanaraman V. S., Robert-Guroff M., Lister T. A., Galton D. A., Sarin P. S., Crawford M. H., Catovsky D., Greaves M., Gallo R. C. The human type-C retrovirus, HTLV, in Blacks from the Caribbean region, and relationship to adult T-cell leukemia/lymphoma. Int J Cancer. 1982 Sep 15;30(3):257–264. doi: 10.1002/ijc.2910300302. [DOI] [PubMed] [Google Scholar]
- Byrne J. A., Butler J. L., Cooper M. D. Differential activation requirements for virgin and memory T cells. J Immunol. 1988 Nov 15;141(10):3249–3257. [PubMed] [Google Scholar]
- De B. K., Srinivasan A. Detection of human immunodeficiency virus (HIV) and human lymphotropic virus (HTLV) type I or II dual infections by polymerase chain reaction. Oncogene. 1989 Dec;4(12):1533–1535. [PubMed] [Google Scholar]
- Imamura N., Inada T., Mtasiwa D. M., Kuramoto A. Phenotype and function of Japanese adult T-cell leukaemia cells. Lancet. 1989 Jul 22;2(8656):214–214. doi: 10.1016/s0140-6736(89)90393-0. [DOI] [PubMed] [Google Scholar]
- Itoyama Y., Kira J., Fujii N., Goto I., Yamamoto N. Increases in helper inducer T cells and activated T cells in HTLV-I-associated myelopathy. Ann Neurol. 1989 Aug;26(2):257–262. doi: 10.1002/ana.410260212. [DOI] [PubMed] [Google Scholar]
- Itoyama Y., Minato S., Kira J., Goto I., Sato H., Okochi K., Yamamoto N. Altered subsets of peripheral blood lymphocytes in patients with HTLV-I associated myelopathy (HAM). Neurology. 1988 May;38(5):816–818. doi: 10.1212/wnl.38.5.816. [DOI] [PubMed] [Google Scholar]
- Jacobson S., Gupta A., Mattson D., Mingioli E., McFarlin D. E. Immunological studies in tropical spastic paraparesis. Ann Neurol. 1990 Feb;27(2):149–156. doi: 10.1002/ana.410270209. [DOI] [PubMed] [Google Scholar]
- Kalish R. S., Morimoto C., Schlossman S. F. Generation of CD8 (T8) cytotoxic cells has a preferential requirement for CD4+2H4- inducer cells. Cell Immunol. 1988 Feb;111(2):379–389. doi: 10.1016/0008-8749(88)90101-3. [DOI] [PubMed] [Google Scholar]
- Klimas N. G., Blaney N. T., Morgan R. O., Chitwood D., Milles K., Lee H., Fletcher M. A. Immune function and anti-HTLV-I/II status in anti-HIV-1-negative intravenous drug users receiving methadone. Am J Med. 1991 Feb;90(2):163–170. [PubMed] [Google Scholar]
- Lairmore M. D., Jacobson S., Gracia F., De B. K., Castillo L., Larreategui M., Roberts B. D., Levine P. H., Blattner W. A., Kaplan J. E. Isolation of human T-cell lymphotropic virus type 2 from Guaymi Indians in Panama. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8840–8844. doi: 10.1073/pnas.87.22.8840. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lal R. B., Hira S. K., Dhawan R. R., Perine P. L. Long-term preservation of whole blood samples for flow cytometry analysis in normal and HIV-infected individuals from Africa. Int J STD AIDS. 1990 Jan;1(1):38–45. doi: 10.1177/095646249000100110. [DOI] [PubMed] [Google Scholar]
- Lal R. B., Rudolph D. L., Lairmore M. D., Khabbaz R. F., Garfield M., Coligan J. E., Folks T. M. Serologic discrimination of human T cell lymphotropic virus infection by using a synthetic peptide-based enzyme immunoassay. J Infect Dis. 1991 Jan;163(1):41–46. doi: 10.1093/infdis/163.1.41. [DOI] [PubMed] [Google Scholar]
- Lal R. B., Rudolph D. L., Roberts C., Honda M. Elevated levels of soluble CD8 and soluble CD25 in patients with human T cell leukemia virus type I-associated myelopathy and adult T cell leukemia. J Infect Dis. 1991 Aug;164(2):429–430. doi: 10.1093/infdis/164.2.429. [DOI] [PubMed] [Google Scholar]
- Morimoto C., Letvin N. L., Boyd A. W., Hagan M., Brown H. M., Kornacki M. M., Schlossman S. F. The isolation and characterization of the human helper inducer T cell subset. J Immunol. 1985 Jun;134(6):3762–3769. [PubMed] [Google Scholar]
- Morimoto C., Letvin N. L., Distaso J. A., Aldrich W. R., Schlossman S. F. The isolation and characterization of the human suppressor inducer T cell subset. J Immunol. 1985 Mar;134(3):1508–1515. [PubMed] [Google Scholar]
- Morimoto C., Rudd C. E., Letvin N. L., Schlossman S. F. A novel epitope of the LFA-1 antigen which can distinguish killer effector and suppressor cells in human CD8 cells. Nature. 1987 Dec 3;330(6147):479–482. doi: 10.1038/330479a0. [DOI] [PubMed] [Google Scholar]
- Osame M., Matsumoto M., Usuku K., Izumo S., Ijichi N., Amitani H., Tara M., Igata A. Chronic progressive myelopathy associated with elevated antibodies to human T-lymphotropic virus type I and adult T-cell leukemialike cells. Ann Neurol. 1987 Feb;21(2):117–122. doi: 10.1002/ana.410210203. [DOI] [PubMed] [Google Scholar]
- Osame M., Usuku K., Izumo S., Ijichi N., Amitani H., Igata A., Matsumoto M., Tara M. HTLV-I associated myelopathy, a new clinical entity. Lancet. 1986 May 3;1(8488):1031–1032. doi: 10.1016/s0140-6736(86)91298-5. [DOI] [PubMed] [Google Scholar]
- Powrie F., Mason D. Phenotypic and functional heterogeneity of CD4+ T cells. Immunol Today. 1988 Sep;9(9):274–277. doi: 10.1016/0167-5699(88)91309-6. [DOI] [PubMed] [Google Scholar]
- Prince H. E. American blood donors seropositive for human T-lymphotropic virus types I/II exhibit normal lymphocyte subsets. Transfusion. 1990 Nov-Dec;30(9):787–790. doi: 10.1046/j.1537-2995.1990.30991048783.x. [DOI] [PubMed] [Google Scholar]
- Richardson J. H., Edwards A. J., Cruickshank J. K., Rudge P., Dalgleish A. G. In vivo cellular tropism of human T-cell leukemia virus type 1. J Virol. 1990 Nov;64(11):5682–5687. doi: 10.1128/jvi.64.11.5682-5687.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenblatt J. D., Plaeger-Marshall S., Giorgi J. V., Swanson P., Chen I. S., Chin E., Wang H. J., Canavaggio M., Hausner M. A., Black A. C. A clinical, hematologic, and immunologic analysis of 21 HTLV-II-infected intravenous drug users. Blood. 1990 Jul 15;76(2):409–417. [PubMed] [Google Scholar]
- Rudd C. E., Anderson P., Morimoto C., Streuli M., Schlossman S. F. Molecular interactions, T-cell subsets and a role of the CD4/CD8:p56lck complex in human T-cell activation. Immunol Rev. 1989 Oct;111:225–266. doi: 10.1111/j.1600-065x.1989.tb00548.x. [DOI] [PubMed] [Google Scholar]
- Sanders M. E., Makgoba M. W., Sharrow S. O., Stephany D., Springer T. A., Young H. A., Shaw S. Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29, and Pgp-1) and have enhanced IFN-gamma production. J Immunol. 1988 Mar 1;140(5):1401–1407. [PubMed] [Google Scholar]
- Shaw G. M., Broder S., Essex M., Gallo R. C. Human T-cell leukemia virus: its discovery and role in leukemogenesis and immunosuppression. Adv Intern Med. 1984;30:1–27. [PubMed] [Google Scholar]
- Sohen S., Rothstein D. M., Tallman T., Gaudette D., Schlossman S. F., Morimoto C. The functional heterogeneity of CD8+ cells defined by anti-CD45RA (2H4) and anti-CD29 (4B4) antibodies. Cell Immunol. 1990 Jun;128(1):314–328. doi: 10.1016/0008-8749(90)90028-p. [DOI] [PubMed] [Google Scholar]
- Tanaka Y., Oda S., Nagata K., Mori N., Sakamoto H., Eto S., Yamashita U. Immunological functions and phenotypes of peripheral blood lymphocytes from human T-cell leukemia virus-I carriers. J Clin Immunol. 1989 Nov;9(6):477–484. doi: 10.1007/BF00918017. [DOI] [PubMed] [Google Scholar]
- Tendler C. L., Greenberg S. J., Blattner W. A., Manns A., Murphy E., Fleisher T., Hanchard B., Morgan O., Burton J. D., Nelson D. L. Transactivation of interleukin 2 and its receptor induces immune activation in human T-cell lymphotropic virus type I-associated myelopathy: pathogenic implications and a rationale for immunotherapy. Proc Natl Acad Sci U S A. 1990 Jul;87(13):5218–5222. doi: 10.1073/pnas.87.13.5218. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomkinson B. E., Brown M. C., Ip S. H., Carrabis S., Sullivan J. L. Soluble CD8 during T cell activation. J Immunol. 1989 Apr 1;142(7):2230–2236. [PubMed] [Google Scholar]
- Yasuda K., Sei Y., Yokoyama M. M., Tanaka K., Hara A. Healthy HTLV-I carriers in Japan: the haematological and immunological characteristics. Br J Haematol. 1986 Sep;64(1):195–203. doi: 10.1111/j.1365-2141.1986.tb07587.x. [DOI] [PubMed] [Google Scholar]
- deShazo R. D., Chadha N., Morgan J. E., Shorty V. J., Rangan S. R., Kalyanaraman V. S., Hyslop N., Chapman Y., O'Dea S. Immunologic assessment of a cluster of asymptomatic HTLV-I-infected individuals in New Orleans. Am J Med. 1989 Jan;86(1):65–70. doi: 10.1016/0002-9343(89)90231-3. [DOI] [PubMed] [Google Scholar]