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. 1992 May;88(2):345–349. doi: 10.1111/j.1365-2249.1992.tb03085.x

Isolation and characterization of dendritic cells from adenoids of children with otitis media with effusion.

E B van Nieuwkerk 1, S van der Baan 1, C D Richters 1, E W Kamperdijk 1
PMCID: PMC1554297  PMID: 1572100

Abstract

Dendritic cells were enriched from adenoids of children with otitis media with effusion (OME) by density gradient centrifugation and culture techniques. An enrichment of 40-140-fold was obtained for dendritic cells. These cells were identified using morphology, enzyme cytochemistry, immunocytochemistry and functional criteria. Dendritic cells could be easily distinguished from macrophages. It appeared that the MoAb EBM11 (CD68) discriminated between dendritic cells and macrophages; in dendritic cells this activity was localized in a spot, whereas in macrophages it was found throughout the whole cytoplasm. The fractions enriched with dendritic cells showed a strong stimulatory effect on allogeneic T cells. These responses were MHC class II dependent since they could be blocked by anti-HLA-DR/DQ MoAbs. The data clearly show that dendritic cells from adenoids of children with OME still have functional capacities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  2. Betjes M. G., Haks M. C., Tuk C. W., Beelen R. H. Monoclonal antibody EBM11 (anti-CD68) discriminates between dendritic cells and macrophages after short-term culture. Immunobiology. 1991 Sep;183(1-2):79–87. doi: 10.1016/S0171-2985(11)80187-7. [DOI] [PubMed] [Google Scholar]
  3. Corradi M. P., Jelinek D. F., Ramberg J. E., Lipsky P. E. Development of a cell with dendritic morphology from a precursor of B lymphocyte lineage. J Immunol. 1987 Apr 1;138(7):2075–2081. [PubMed] [Google Scholar]
  4. Gates G. A., Avery C. A., Prihoda T. J. Effect of adenoidectomy upon children with chronic otitis media with effusion. Laryngoscope. 1988 Jan;98(1):58–63. doi: 10.1288/00005537-198801000-00013. [DOI] [PubMed] [Google Scholar]
  5. Hart D. N., McKenzie J. L. Isolation and characterization of human tonsil dendritic cells. J Exp Med. 1988 Jul 1;168(1):157–170. doi: 10.1084/jem.168.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kamperdijk E. W., Kapsenberg M. L., van den Berg M., Hoefsmit E. C. Characterization of dendritic cells, isolated from normal and stimulated lymph nodes of the rat. Cell Tissue Res. 1985;242(3):469–474. doi: 10.1007/BF00225411. [DOI] [PubMed] [Google Scholar]
  7. Kelly P. M., Bliss E., Morton J. A., Burns J., McGee J. O. Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol. 1988 May;41(5):510–515. doi: 10.1136/jcp.41.5.510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. King P. D., Katz D. R. Human tonsillar dendritic cell-induced T cell responses: analysis of molecular mechanisms using monoclonal antibodies. Eur J Immunol. 1989 Apr;19(4):581–587. doi: 10.1002/eji.1830190402. [DOI] [PubMed] [Google Scholar]
  9. Landry D., Lafontaine M., Cossette M., Barthélémy H., Chartrand C., Montplaisir S., Pelletier M. Human thymic dendritic cells. Characterization, isolation and functional assays. Immunology. 1988 Sep;65(1):135–142. [PMC free article] [PubMed] [Google Scholar]
  10. Le J., Yao J. S., Knowles D. M., 2nd, Vilcek J. Accessory function of thymic and tonsillar dendritic cells in interferon gamma production by T lymphocytes. Lymphokine Res. 1986 Summer;5(3):205–213. [PubMed] [Google Scholar]
  11. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  12. Schuler G., Steinman R. M. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J Exp Med. 1985 Mar 1;161(3):526–546. doi: 10.1084/jem.161.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sprinkle P. M., Veltri R. W. Pathophysiology of serous otitis media. Am J Otol. 1986 Mar;7(2):113–118. [PubMed] [Google Scholar]
  14. Teunissen M. B., Wormmeester J., Krieg S. R., Peters P. J., Vogels I. M., Kapsenberg M. L., Bos J. D. Human epidermal Langerhans cells undergo profound morphologic and phenotypical changes during in vitro culture. J Invest Dermatol. 1990 Feb;94(2):166–173. doi: 10.1111/1523-1747.ep12874439. [DOI] [PubMed] [Google Scholar]
  15. Tew J. G., Thorbecke G. J., Steinman R. M. Dendritic cells in the immune response: characteristics and recommended nomenclature (A report from the Reticuloendothelial Society Committee on Nomenclature). J Reticuloendothel Soc. 1982 May;31(5):371–380. [PubMed] [Google Scholar]
  16. Unanue E. R., Beller D. I., Lu C. Y., Allen P. M. Antigen presentation: comments on its regulation and mechanism. J Immunol. 1984 Jan;132(1):1–5. [PubMed] [Google Scholar]
  17. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Waalen K., Thoen J., Førre O., Hovig T., Teigland J., Natvig J. B. Rheumatoid synovial dendritic cells as stimulators in allogeneic and autologous mixed leukocyte reactions--comparison with autologous monocytes as stimulator cells. Scand J Immunol. 1986 Feb;23(2):233–241. doi: 10.1111/j.1365-3083.1986.tb01962.x. [DOI] [PubMed] [Google Scholar]
  19. de Vere Tyndall A., Knight S. C., Edwards A. J., Clarke J. B. Veiled (dendritic) cells in synovial fluid. Lancet. 1983 Feb 26;1(8322):472–473. doi: 10.1016/s0140-6736(83)91468-x. [DOI] [PubMed] [Google Scholar]
  20. van Nieuwkerk E. B., Kamperdijk E. W., Verdaasdonk M. A., van der Baan S., Hoefsmit E. C. Langerhans cells in the respiratory epithelium of the human adenoid. Eur J Cell Biol. 1991 Feb;54(1):182–186. [PubMed] [Google Scholar]
  21. van Nieuwkerk E. B., de Wolf C. J., Kamperdijk E. W., van der Baan S. Lymphoid and non-lymphoid cells in the adenoid of children with otitis media with effusion: a comparative study. Clin Exp Immunol. 1990 Feb;79(2):233–239. doi: 10.1111/j.1365-2249.1990.tb05184.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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