Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1992 Mar;87(3):461–464. doi: 10.1111/j.1365-2249.1992.tb03020.x

Effect of 12,13-phorbol dibutyrate and ionomycin on defective B cells in common variable immunodeficiency.

A Franz 1, A Bryant 1, A D Webster 1, J Farrant 1
PMCID: PMC1554328  PMID: 1544230

Abstract

Secretion of IgM and IgG in vitro by B cells from patients with common variable immunodeficiency (CVI) has been used to classify the disease into three groups. On stimulation with anti-IgM and IL-2, group A patients' cells fail to secrete IgM or IgG, group B patients' cells secrete no IgG and significantly lower levels of IgM than normal cells, and group C patients' cells produce normal levels of both isotypes. Direct activation of protein kinase C using 12,13-phorbol dibutyrate and ionomycin followed by IL-2 or IL-4 has been reported to induce immunoglobulin secretion by normal human B cells. We therefore attempted to induce B cells from group A and group B CVI patients to secrete IgM and IgG after direct activation of protein kinase C together with IL-2 or IL-4. The data show that the failure of secretion of immunoglobulin by B cells from CVI patients could not be reversed using this approach. This finding suggests that the activation channel involving protein kinase C in B cells from CVI patients is not involved in the defect in cell differentiation.

Full text

PDF
461

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bryant A., Calver N. C., Toubi E., Webster A. D., Farrant J. Classification of patients with common variable immunodeficiency by B cell secretion of IgM and IgG in response to anti-IgM and interleukin-2. Clin Immunol Immunopathol. 1990 Aug;56(2):239–248. doi: 10.1016/0090-1229(90)90145-g. [DOI] [PubMed] [Google Scholar]
  2. Farrant J., Bryant A. E., Lever A. M., Edwards A. J., Knight S. C., Webster A. D. Defective low-density cells of dendritic morphology from the blood of patients with common variable hypogammaglobulinaemia: low immunoglobulin production on stimulation of normal B cells. Clin Exp Immunol. 1985 Jul;61(1):189–194. [PMC free article] [PubMed] [Google Scholar]
  3. Farrant J., Bryant A., Almandoz F., Spickett G., Evans S. W., Webster A. D. B cell function in acquired "common-variable" hypogammaglobulinemia: proliferative responses to lymphokines. Clin Immunol Immunopathol. 1989 May;51(2):196–204. doi: 10.1016/0090-1229(89)90019-6. [DOI] [PubMed] [Google Scholar]
  4. Farrant J., Clark J. C., Lee H., Knight S. C., O'Brien J. Conditions for measuring DNA synthesis in PHA stimulated human lymphocytes in 20 microliters hanging drops with various cell concentrations and periods of culture. J Immunol Methods. 1980;33(4):301–312. doi: 10.1016/0022-1759(80)90001-0. [DOI] [PubMed] [Google Scholar]
  5. Flores-Romo L., Millsum M. J., Gillis S., Stubbs P., Sykes C., Gordon J. Immunoglobulin isotype production by cycling human B lymphocytes in response to recombinant cytokines and anti-IgM. Immunology. 1990 Mar;69(3):342–347. [PMC free article] [PubMed] [Google Scholar]
  6. Franz A., Bryant A., Farrant J. Interleukin-2-induced DNA synthesis and immunoglobulin secretion by resting human tonsillar B cells: effects of protein kinase C activation. Immunology. 1991 Jul;73(3):322–326. [PMC free article] [PubMed] [Google Scholar]
  7. Knight S. C., Balfour B. M., O'Brien J., Buttifant L., Sumerska T., Clarke J. Role of veiled cells in lymphocyte activation. Eur J Immunol. 1982 Dec;12(12):1057–1060. doi: 10.1002/eji.1830121214. [DOI] [PubMed] [Google Scholar]
  8. North M. E., Spickett G. P., Allsop J., Webster A. D., Farrant J. Defective DNA synthesis by T cells in acquired 'common-variable' hypogammaglobulinaemia on stimulation with mitogens. Clin Exp Immunol. 1989 Apr;76(1):19–23. [PMC free article] [PubMed] [Google Scholar]
  9. North M. E., Spickett G. P., Webster A. D., Farrant J. Raised serum levels of CD8, CD25 and beta 2-microglobulin in common variable immunodeficiency. Clin Exp Immunol. 1991 Nov;86(2):252–255. doi: 10.1111/j.1365-2249.1991.tb05805.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. North M. E., Webster A. D., Farrant J. Defects in proliferative responses of T cells from patients with common variable immunodeficiency on direct activation of protein kinase C. Clin Exp Immunol. 1991 Aug;85(2):198–201. doi: 10.1111/j.1365-2249.1991.tb05704.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. North M. E., Webster A. D., Farrant J. Role of interleukin-2 and interleukin-6 in the mitogen responsiveness of T cells from patients with 'common-variable' hypogammaglobulinaemia. Clin Exp Immunol. 1990 Sep;81(3):412–416. doi: 10.1111/j.1365-2249.1990.tb05348.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Primary immunodeficiency diseases. Report of a WHO sponsored meeting. Immunodefic Rev. 1989;1(2):173–205. [PubMed] [Google Scholar]
  13. Roifman C. M., Mills G. B., Stewart D., Cheung R. K., Grinstein S., Gelfand E. W. Response of human B cells to different anti-immunoglobulin isotypes: absence of a correlation between early activation events and cell proliferation. Eur J Immunol. 1987 Dec;17(12):1737–1742. doi: 10.1002/eji.1830171209. [DOI] [PubMed] [Google Scholar]
  14. Spickett G. P., Webster A. D., Farrant J. Cellular abnormalities in common variable immunodeficiency. Immunodefic Rev. 1990;2(3):199–219. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES