Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1992 Jul;89(1):38–45. doi: 10.1111/j.1365-2249.1992.tb06874.x

IgG antibodies from patients with primary Sjögren's syndrome and systemic lupus erythematosus recognize different epitopes in 60-kD SSA/Ro protein.

S Barakat 1, O Meyer 1, F Torterotot 1, P Youinou 1, J P Briand 1, M F Kahn 1, S Muller 1
PMCID: PMC1554418  PMID: 1378364

Abstract

Five synthetic peptides corresponding to the N-, the C- and a central domain in 60-kD SSA/Ro protein were prepared and tested with sera from 112 patients with systemic lupus erythematosus (SLE), 55 with primary Sjögren's syndrome (pSS) and 29 with rheumatoid arthritis. Among these five fragments, one representing residues 21-41, was recognized by antibodies in 57% of pSS patients. Interestingly, this peptide was recognized by only a few (less than or equal to 7%) of SLE sera, while 63% of pSS sera and 46% of SLE sera tested in parallel possessed antibodies reacting in ELISA with purified 60-kD SSA protein. The ELISA results were compared with the pattern of reactivity obtained in immunodiffusion and immunoblotting. The results indicate that the sensitivity of ELISA using peptide 21-41 and pSS sera was in the same range as immunoblotting and higher than immunodiffusion. Thus the peptide 21-41 proved useful for the detection of anti-SSA antibodies in the sera of patients with pSS. Furthermore, a positive ELISA using peptide 21-41 could be of potential use to discriminate pSS with systemic features from SLE. The fact that peptide 21-41 is recognized by antibodies in pSS but only by very few SLE sera implies that different mechanisms are involved in the anti-SSA immune response in these two autoimmune diseases.

Full text

PDF
38

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barakat S., Briand J. P., Abuaf N., van Regenmortel M. H., Muller S. Mapping of epitopes on U1 snRNP polypeptide A with synthetic peptides and autoimmune sera. Clin Exp Immunol. 1991 Oct;86(1):71–78. doi: 10.1111/j.1365-2249.1991.tb05776.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barakat S., Briand J. P., Weber J. C., van Regenmortel M. H., Muller S. Recognition of synthetic peptides of Sm-D autoantigen by lupus sera. Clin Exp Immunol. 1990 Aug;81(2):256–262. doi: 10.1111/j.1365-2249.1990.tb03327.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ben-Chetrit E., Chan E. K., Sullivan K. F., Tan E. M. A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med. 1988 May 1;167(5):1560–1571. doi: 10.1084/jem.167.5.1560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Briand J. P., Van Dorsselaer A., Raboy B., Muller S. Total chemical synthesis of ubiquitin using BOP reagent: biochemical and immunochemical properties of the purified synthetic product. Pept Res. 1989 Nov-Dec;2(6):381–388. [PubMed] [Google Scholar]
  5. Buyon J. P., Ben-Chetrit E., Karp S., Roubey R. A., Pompeo L., Reeves W. H., Tan E. M., Winchester R. Acquired congenital heart block. Pattern of maternal antibody response to biochemically defined antigens of the SSA/Ro-SSB/La system in neonatal lupus. J Clin Invest. 1989 Aug;84(2):627–634. doi: 10.1172/JCI114208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark G., Reichlin M., Tomasi T. B., Jr Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythmatosus. J Immunol. 1969 Jan;102(1):117–122. [PubMed] [Google Scholar]
  7. Deutscher S. L., Harley J. B., Keene J. D. Molecular analysis of the 60-kDa human Ro ribonucleoprotein. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9479–9483. doi: 10.1073/pnas.85.24.9479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Horsfall A. C., Venables P. J., Taylor P. V., Maini R. N. Ro and La antigens and maternal anti-La idiotype on the surface of myocardial fibres in congenital heart block. J Autoimmun. 1991 Feb;4(1):165–176. doi: 10.1016/0896-8411(91)90015-5. [DOI] [PubMed] [Google Scholar]
  9. Itoh Y., Rader M. D., Reichlin M. Heterogeneity of the Ro/SSA antigen and autoanti-Ro/SSA response: evidence of the four antigenically distinct forms. Clin Exp Immunol. 1990 Jul;81(1):45–51. doi: 10.1111/j.1365-2249.1990.tb05289.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meyer O., Bourgeois P., Aeschlimann A., Haim T., Mery J. P., Kahn M. F. Immunoblotting profiles in 55 systemic lupus erythematosus sera lacking precipitating antibodies to extractable nuclear antigens. Ann Rheum Dis. 1989 Jul;48(7):594–599. doi: 10.1136/ard.48.7.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meyer O., Hauptmann G., Tappeiner G., Ochs H. D., Mascart-Lemone F. Genetic deficiency of C4, C2 or C1q and lupus syndromes. Association with anti-Ro (SS-A) antibodies. Clin Exp Immunol. 1985 Dec;62(3):678–684. [PMC free article] [PubMed] [Google Scholar]
  12. Muller S., Barakat S., Watts R., Joubaud P., Isenberg D. Longitudinal analysis of antibodies to histones, Sm-D peptides and ubiquitin in the serum of patients with systemic lupus erythematosus, rheumatoid arthritis and tuberculosis. Clin Exp Rheumatol. 1990 Sep-Oct;8(5):445–453. [PubMed] [Google Scholar]
  13. Plaué S., Muller S., van Regenmortel M. H. A branched, synthetic octapeptide of ubiquitinated histone H2A as target of autoantibodies. J Exp Med. 1989 May 1;169(5):1607–1617. doi: 10.1084/jem.169.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reichlin M. Significance of the Ro antigen system. J Clin Immunol. 1986 Sep;6(5):339–348. doi: 10.1007/BF00915372. [DOI] [PubMed] [Google Scholar]
  15. Scofield R. H., Harley J. B. Autoantigenicity of Ro/SSA antigen is related to a nucleocapsid protein of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3343–3347. doi: 10.1073/pnas.88.8.3343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smeenk R., Westgeest T., Swaak T. Antinuclear antibody determination: the present state of diagnostic and clinical relevance. Scand J Rheumatol Suppl. 1985;56:78–92. doi: 10.3109/03009748509102067. [DOI] [PubMed] [Google Scholar]
  17. Tan E. M. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol. 1989;44:93–151. doi: 10.1016/s0065-2776(08)60641-0. [DOI] [PubMed] [Google Scholar]
  18. Tuaillon N., Muller S., Pasquali J. L., Bordigoni P., Youinou P., Van Regenmortel M. H. Antibodies from patients with rheumatoid arthritis and juvenile chronic arthritis analyzed with core histone synthetic peptides. Int Arch Allergy Appl Immunol. 1990;91(3):297–305. doi: 10.1159/000235131. [DOI] [PubMed] [Google Scholar]
  19. Wolin S. L., Steitz J. A. The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1996–2000. doi: 10.1073/pnas.81.7.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES