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. 1992 Aug;89(2):315–320. doi: 10.1111/j.1365-2249.1992.tb06952.x

IgA-containing immune complexes after challenge with food antigens in patients with IgA nephropathy.

S Jackson 1, Z Moldoveanu 1, K A Kirk 1, B A Julian 1, T F Patterson 1, A L Mullins 1, T Jilling 1, J Mestecky 1, J H Galla 1
PMCID: PMC1554422  PMID: 1638775

Abstract

The possibility that patients with IgA nephropathy (IgAN) might have abnormal IgA immune responses to immunogens commonly encountered at mucosal surfaces, resulting in the formation of circulating immune complexes (CIC), was examined. Since it is generally held that such increased IgA responses are characterized by detectable aberrancies in handling of IgA-containing CIC, IgAN patients and controls were given a large volume of bovine milk (after dietary deprivation of bovine antigens) and immune complex levels were measured over a period of 12 h. An assay based on binding of CIC containing C3 to solid-phase anti-C3 and subsequent development with isotype-specific antibody revealed no differences in responses of patients and controls with respect to IgG- and IgM-containing CIC. Although IgAN patients tended to have higher levels of IgA-containing CIC, there were no differences in response patterns when IgA CIC levels after ingestion of the milk stimulus were related to baseline levels. Polymorphonuclear leucocytes (PMNC), which bear surface receptors for IgA, were isolated from some subjects at the same times as the samples for CIC levels and examined by two-colour immunofluorescence for the coincident presence of IgA and milk antigens. In contrast to the data obtained in the CIC assays, these experiments revealed the simultaneous presence of IgA and two of three milk proteins in PMNC of IgAN patients but not controls. Follow-up experiments designed to assess more quantitatively the coincidental presence of IgA and milk antigens indicated no significant differences between patients and controls. However, milk proteins seemed to be more commonly associated with IgA in PMNC of IgAN patients, suggesting the presence of non-complement-fixing IgA/antigen CIC after mucosal challenge of some IgAN patients.

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Selected References

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  1. Abu-Ghazaleh R. I., Fujisawa T., Mestecky J., Kyle R. A., Gleich G. J. IgA-induced eosinophil degranulation. J Immunol. 1989 Apr 1;142(7):2393–2400. [PubMed] [Google Scholar]
  2. Barton J. C., Parmley R. T. Light microscopic, non-immunologic demonstration of iron-binding proteins in hematopoietic cells. J Histochem Cytochem. 1986 Mar;34(3):299–305. doi: 10.1177/34.3.3950383. [DOI] [PubMed] [Google Scholar]
  3. Cairns S. A., London A., Mallick N. P. Circulating immune complexes following food: delayed clearance in idiopathic glomerulonephritis. J Clin Lab Immunol. 1981 Sep;6(2):121–126. [PubMed] [Google Scholar]
  4. Coppo R., Basolo B., Rollino C., Roccatello D., Martina G., Amore A., Bongiorno G., Piccoli G. Mediterranean diet and primary IgA nephropathy. Clin Nephrol. 1986 Aug;26(2):72–82. [PubMed] [Google Scholar]
  5. Coppo R., Mazzucco G., Martina G., Roccatello D., Amore A., Novara R., Bargoni A., Piccoli G., Sena L. M. Gluten-induced experimental IgA glomerulopathy. Lab Invest. 1989 Apr;60(4):499–506. [PubMed] [Google Scholar]
  6. Czerkinsky C., Koopman W. J., Jackson S., Collins J. E., Crago S. S., Schrohenloher R. E., Julian B. A., Galla J. H., Mestecky J. Circulating immune complexes and immunoglobulin A rheumatoid factor in patients with mesangial immunoglobulin A nephropathies. J Clin Invest. 1986 Jun;77(6):1931–1938. doi: 10.1172/JCI112522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. D'Amico G. The commonest glomerulonephritis in the world: IgA nephropathy. Q J Med. 1987 Sep;64(245):709–727. [PubMed] [Google Scholar]
  8. Emancipator S. N., Gallo G. R., Lamm M. E. Experimental IgA nephropathy induced by oral immunization. J Exp Med. 1983 Feb 1;157(2):572–582. doi: 10.1084/jem.157.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Emancipator S. N., Gallo G. R., Lamm M. E. IgA nephropathy: perspectives on pathogenesis and classification. Clin Nephrol. 1985 Oct;24(4):161–179. [PubMed] [Google Scholar]
  10. Emancipator S. N., Lamm M. E. IgA nephropathy: pathogenesis of the most common form of glomerulonephritis. Lab Invest. 1989 Feb;60(2):168–183. [PubMed] [Google Scholar]
  11. Fanger M. W., Goldstine S. N., Shen L. The properties and role of receptors for IgA on human leukocytes. Ann N Y Acad Sci. 1983 Jun 30;409:552–563. doi: 10.1111/j.1749-6632.1983.tb26898.x. [DOI] [PubMed] [Google Scholar]
  12. Feehally J., Beattie T. J., Brenchley P. E., Coupes B. M., Mallick N. P., Postlethwaite R. J. Response of circulating immune complexes to food challenge in relapsing IgA nephropathy. Pediatr Nephrol. 1987 Oct;1(4):581–586. doi: 10.1007/BF00853592. [DOI] [PubMed] [Google Scholar]
  13. Feehally J. Immune mechanisms in glomerular IgA deposition. Nephrol Dial Transplant. 1988;3(4):361–378. doi: 10.1093/oxfordjournals.ndt.a091683. [DOI] [PubMed] [Google Scholar]
  14. Genin C., Laurent B., Sabatier J. C., Colon S., Berthoux F. C. IgA mesangial deposits in C3H/HeJ mice after oral immunization with ferritin or bovine serum albumin. Clin Exp Immunol. 1986 Feb;63(2):385–394. [PMC free article] [PubMed] [Google Scholar]
  15. Gregory M. C., Hammond M. E., Brewer E. D. Renal deposition of cytomegalovirus antigen in immunoglobulin-A nephropathy. Lancet. 1988 Jan 2;1(8575-6):11–14. doi: 10.1016/s0140-6736(88)91000-8. [DOI] [PubMed] [Google Scholar]
  16. Jackson S., Montgomery R. I., Julian B. A., Galla J. H., Czerkinsky C. Aberrant synthesis of antibodies directed at the Fab fragment of IgA in patients with IgA nephropathies. Clin Immunol Immunopathol. 1987 Nov;45(2):208–213. doi: 10.1016/0090-1229(87)90035-3. [DOI] [PubMed] [Google Scholar]
  17. Jessen R. H., Nedrud J. G., Emancipator S. N. A mouse model of IgA nephropathy induced by Sendai virus. Adv Exp Med Biol. 1987;216B:1609–1618. [PubMed] [Google Scholar]
  18. Kincaid-Smith P., Nicholls K., Birchall I. Polymorphs infiltrate glomeruli in mesangial IgA glomerulonephritis. Kidney Int. 1989 Dec;36(6):1108–1111. doi: 10.1038/ki.1989.308. [DOI] [PubMed] [Google Scholar]
  19. Monteiro R. C., Kubagawa H., Cooper M. D. Cellular distribution, regulation, and biochemical nature of an Fc alpha receptor in humans. J Exp Med. 1990 Mar 1;171(3):597–613. doi: 10.1084/jem.171.3.597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nagy J., Uj M., Szücs G., Trinn C., Burger T. Herpes virus antigens and antibodies in kidney biopsies and sera of IgA glomerulonephritic patients. Clin Nephrol. 1984 May;21(5):259–262. [PubMed] [Google Scholar]
  21. Paganelli R., Atherton D. J., Levinsky R. J. Differences between normal and milk allergic subjects in their immune responses after milk ingestion. Arch Dis Child. 1983 Mar;58(3):201–206. doi: 10.1136/adc.58.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Russell M. W., Brown T. A., Mestecky J. Role of serum IgA. Hepatobiliary transport of circulating antigen. J Exp Med. 1981 Apr 1;153(4):968–976. doi: 10.1084/jem.153.4.968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Russell M. W., Mestecky J., Julian B. A., Galla J. H. IgA-associated renal diseases: antibodies to environmental antigens in sera and deposition of immunoglobulins and antigens in glomeruli. J Clin Immunol. 1986 Jan;6(1):74–86. doi: 10.1007/BF00915367. [DOI] [PubMed] [Google Scholar]
  24. Russell M. W., Spotswood M. F., Julian B. A., Galla J. H., Mestecky J. Detection of food antigen-specific IgA immune complexes in human sera. Adv Exp Med Biol. 1987;216A:813–820. doi: 10.1007/978-1-4684-5344-7_94. [DOI] [PubMed] [Google Scholar]
  25. Sancho J., Egido J., Rivera F., Hernando L. Immune complexes in IgA nephropathy: presence of antibodies against diet antigens and delayed clearance of specific polymeric IgA immune complexes. Clin Exp Immunol. 1983 Oct;54(1):194–202. [PMC free article] [PubMed] [Google Scholar]
  26. Sato M., Kojima H., Takayama K., Koshikawa S. Glomerular deposition of food antigens in IgA nephropathy. Clin Exp Immunol. 1988 Aug;73(2):295–299. [PMC free article] [PubMed] [Google Scholar]
  27. Sato M., Takayama K., Wakasa M., Koshikawa S. Estimation of circulating immune complexes following oral challenge with cow's milk in patients with IgA nephropathy. Nephron. 1987;47(1):43–48. doi: 10.1159/000184455. [DOI] [PubMed] [Google Scholar]
  28. Tomana M., Kulhavy R., Mestecky J. Receptor-mediated binding and uptake of immunoglobulin A by human liver. Gastroenterology. 1988 Mar;94(3):762–770. doi: 10.1016/0016-5085(88)90252-1. [DOI] [PubMed] [Google Scholar]
  29. Tomino Y., Endoh M., Nomoto Y., Sakai H. Specificity of eluted antibody from renal tissues of patients with IgA nephropathy. Am J Kidney Dis. 1982 Mar;1(5):276–280. doi: 10.1016/s0272-6386(82)80025-5. [DOI] [PubMed] [Google Scholar]
  30. van der Woude F. J., Hoedemaeker P. J., van der Giessen M., de Graeff P. A., de Monchy J., The T. H., van der Hem G. K. Do food antigens play a role in the pathogenesis of some cases of human glomerulonephritis? Clin Exp Immunol. 1983 Mar;51(3):587–594. [PMC free article] [PubMed] [Google Scholar]

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