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. 1992 Jun;88(3):543–547. doi: 10.1111/j.1365-2249.1992.tb06484.x

Alpha-1 anti-trypsin and CD30 expression occur in parallel in activated T cells.

M S Bashir 1, D B Jones 1, D H Wright 1
PMCID: PMC1554524  PMID: 1318806

Abstract

We have performed an immunohistochemical study of immunoblastic T cell lymphoma and enteropathy-associated T cell lymphoma for alpha-1 anti-trypsin and CD30. Cytoplasmic staining for alpha 1 anti-trypsin is present in the malignant cells in both types of T cell neoplasm which also express CD30, a marker of lymphoid activation. Peripheral blood T lymphocytes on stimulation with mitogen also show granular cytoplasmic expression of alpha 1 anti-trypsin. Time course studies show that this parallels the expression of CD30. Alpha 1 anti-trypsin expression appears therefore to be associated with activation in T cells. Further studies of sub-fractionated T lymphocytes in vitro suggest that the expression of alpha 1 anti-trypsin on activation is not restricted to an individual lymphocyte subset.

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Selected References

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  1. Adams J., Ridley C. M., Collins C. M., Isaacson P. G. Cutaneous lymphoma expressing both T-cell and macrophage features. J R Soc Med. 1984;77 (Suppl 4):12–15. [PMC free article] [PubMed] [Google Scholar]
  2. Ades E. W., Hinson A., Chapuis-Cellier C., Arnaud P. Modulation of the immune response by plasma protease inhibitors. I. Alpha 2-macroglobulin and alpha 1-antitrypsin inhibit natural killing and antibody-dependent cell-mediated cytotoxicity. Scand J Immunol. 1982 Jan;15(1):109–113. doi: 10.1111/j.1365-3083.1982.tb00628.x. [DOI] [PubMed] [Google Scholar]
  3. Bata J., Martin J. P., Revillard J. P. Cell surface protease activity of human lymphocytes; its inhibition by alpha 1-antitrypsin. Experientia. 1981 May 15;37(5):518–519. doi: 10.1007/BF01986172. [DOI] [PubMed] [Google Scholar]
  4. Bhan A. K., Grand R. J., Colten H. R., Alper C. A. Liver in alpha1-antitrypsin deficiency: morphologic observations and in vitro synthesis of alpha1-antitrypsin. Pediatr Res. 1976 Jan;10(1):35–40. doi: 10.1203/00006450-197601000-00007. [DOI] [PubMed] [Google Scholar]
  5. Breit S. N., Robinson J. P., Luckhurst E., Clark P., Penny R. Immunoregulation by alpha 1 antitrypsin. J Clin Lab Immunol. 1982 Feb;7(2):127–131. [PubMed] [Google Scholar]
  6. Breit S. N., Wakefield D., Robinson J. P., Luckhurst E., Clark P., Penny R. The role of alpha 1-antitrypsin deficiency in the pathogenesis of immune disorders. Clin Immunol Immunopathol. 1985 Jun;35(3):363–380. doi: 10.1016/0090-1229(85)90097-2. [DOI] [PubMed] [Google Scholar]
  7. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  8. Cohen A. B. Interrelationships between the human alveolar macrophage and alpha-1-antitrypsin. J Clin Invest. 1973 Nov;52(11):2793–2799. doi: 10.1172/JCI107475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen S. D., Israel E., Spiess-Meier B., Wainberg M. A. Plasminogen activator is an apparent lymphocyte mitogen. J Immunol. 1981 Apr;126(4):1415–1420. [PubMed] [Google Scholar]
  10. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  11. Gadek J. E., Fells G. A., Zimmerman R. L., Rennard S. I., Crystal R. G. Antielastases of the human alveolar structures. Implications for the protease-antiprotease theory of emphysema. J Clin Invest. 1981 Oct;68(4):889–898. doi: 10.1172/JCI110344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gupta P. K., Frost J. K., Geddes S., Aracil B., Davidovski F. Morphological identification of alpha-1-antitrypsin in pulmonary macrophages. Hum Pathol. 1979 May;10(3):345–347. doi: 10.1016/s0046-8177(79)80030-1. [DOI] [PubMed] [Google Scholar]
  13. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  14. Hudig D., Haverty T., Fulcher C., Redelman D., Mendelsohn J. Inhibition of human natural cytotoxicity by macromolecular antiproteases. J Immunol. 1981 Apr;126(4):1569–1574. [PubMed] [Google Scholar]
  15. Isaacson P. G., O'Connor N. T., Spencer J., Bevan D. H., Connolly C. E., Kirkham N., Pollock D. J., Wainscoat J. S., Stein H., Mason D. Y. Malignant histiocytosis of the intestine: a T-cell lymphoma. Lancet. 1985 Sep 28;2(8457):688–691. doi: 10.1016/s0140-6736(85)92930-7. [DOI] [PubMed] [Google Scholar]
  16. Isaacson P., Jones D. B., Millward-Sadler G. H., Judd M. A., Payne S. Alpha-1-antitrypsin in human macrophages. J Clin Pathol. 1981 Sep;34(9):982–990. doi: 10.1136/jcp.34.9.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Isaacson P., Wright D. H., Jones D. B. Malignant lymphoma of true histiocytic (monocyte/macrophage) origin. Cancer. 1983 Jan 1;51(1):80–91. doi: 10.1002/1097-0142(19830101)51:1<80::aid-cncr2820510118>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  18. Isaacson P., Wright D. H., Judd M. A., Mepham B. L. Primary gastrointestinal lymphomas: a classification of 66 cases. Cancer. 1979 May;43(5):1805–1809. doi: 10.1002/1097-0142(197905)43:5<1805::aid-cncr2820430534>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  19. Isaacson P., Wright D. H., Judd M. A., Mepham B. L. Primary gastrointestinal lymphomas: a classification of 66 cases. Cancer. 1979 May;43(5):1805–1809. doi: 10.1002/1097-0142(197905)43:5<1805::aid-cncr2820430534>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  20. Lentini M., Grosso M., Carrozza G., Risitano G. Fibrohistiocytic tumors of soft tissues. An immunohistochemical study of 183 cases. Pathol Res Pract. 1986 Dec;181(6):713–717. doi: 10.1016/S0344-0338(86)80046-2. [DOI] [PubMed] [Google Scholar]
  21. Lipsky J. J., Berninger R. W., Hyman L. R., Talamo R. C. Presence of alpha-1-antitrypsin on mitogen-stimulated human lymphocytes. J Immunol. 1979 Jan;122(1):24–26. [PubMed] [Google Scholar]
  22. Mir R., Kahn L. B., Selzer G. Immunohistochemistry of primary gastrointestinal lymphomas: a study of 76 cases. Histopathology. 1986 Apr;10(4):391–403. doi: 10.1111/j.1365-2559.1986.tb02492.x. [DOI] [PubMed] [Google Scholar]
  23. Norton A. J., Isaacson P. G. An immunocytochemical study of T-cell lymphomas using monoclonal and polyclonal antibodies effective in routinely fixed wax embedded tissues. Histopathology. 1986 Dec;10(12):1243–1260. doi: 10.1111/j.1365-2559.1986.tb02568.x. [DOI] [PubMed] [Google Scholar]
  24. Perlmutter D. H., Cole F. S., Kilbridge P., Rossing T. H., Colten H. R. Expression of the alpha 1-proteinase inhibitor gene in human monocytes and macrophages. Proc Natl Acad Sci U S A. 1985 Feb;82(3):795–799. doi: 10.1073/pnas.82.3.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Redelman D., Hudig D. The mechanism of cell-mediated cytotoxicity. I. Killing by murine cytotoxic T lymphocytes requires cell surface thiols and activated proteases. J Immunol. 1980 Feb;124(2):870–878. [PubMed] [Google Scholar]
  26. Reed J. C., Nowell P. C. Soluble inhibitors of T lymphocyte proliferation: tools for dissecting pathways of T cell activation. Immunol Res. 1988;7(2):93–112. doi: 10.1007/BF02918095. [DOI] [PubMed] [Google Scholar]
  27. Sharp H. L., Bridges R. A., Krivit W., Freier E. F. Cirrhosis associated with alpha-1-antitrypsin deficiency: a previously unrecognized inherited disorder. J Lab Clin Med. 1969 Jun;73(6):934–939. [PubMed] [Google Scholar]
  28. Stein H., Mason D. Y., Gerdes J., O'Connor N., Wainscoat J., Pallesen G., Gatter K., Falini B., Delsol G., Lemke H. The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood. 1985 Oct;66(4):848–858. [PubMed] [Google Scholar]
  29. Turner R. R., Wood G. S., Beckstead J. H., Colby T. V., Horning S. J., Warnke R. A. Histiocytic malignancies. Morphologic, immunologic, and enzymatic heterogeneity. Am J Surg Pathol. 1984 Jul;8(7):485–500. [PubMed] [Google Scholar]
  30. Vischer T. L., Bretz U., Baggiolini M. In vitro stimulation of lymphocytes by neutral proteinases from human polymorphonuclear leukocyte granules. J Exp Med. 1976 Oct 1;144(4):863–872. doi: 10.1084/jem.144.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. van Furth R., Kramps J. A., Diesselhof-den Dulk M. M. Synthesis of alpha 1-anti-trypsin by human monocytes. Clin Exp Immunol. 1983 Mar;51(3):551–557. [PMC free article] [PubMed] [Google Scholar]

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