Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1992 Jun;88(3):559–562. doi: 10.1111/j.1365-2249.1992.tb06487.x

Serum-induced suppression of interferon (IFN) activity. Lack of evidence for the presence of specific autoantibodies to IFN-alpha in normal human sera.

M B Hansen 1, M Svenson 1, K Bendtzen 1
PMCID: PMC1554525  PMID: 1606741

Abstract

IgG antibodies binding to different IFN species have been described in sera of healthy and diseased individuals. Human serum immunoglobulins have also been shown to interfere with IFN bioactivity. To characterize these antibodies, human recombinant IFN-alpha 2A (rIFN-alpha) was radioiodinated, and ligand binding studies were performed in human sera as well as on the human cell line A-549 in the presence of human serum. 125I-rIFN-alpha bound to serum factors of healthy individuals. However, less than 3% of the binding was to IgG and the binding was non-saturable and therefore most likely non-specific. 125I-rIFN-alpha bound to receptors on A-549 cells, and the ligand-receptor complexes appeared to internalize. However, both cell binding and internalization of 125I-rIFN-alpha were independent of the presence of human serum. We conclude that normal human sera do not contain detectable autoantibodies to rIFN-alpha.

Full text

PDF
559

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguet M., Mogensen K. E. Interferon receptors. Interferon. 1983;5:1–22. [PubMed] [Google Scholar]
  2. Bendtzen K., Svenson M., Jønsson V., Hippe E. Autoantibodies to cytokines--friends or foes? Immunol Today. 1990 May;11(5):167–169. doi: 10.1016/0167-5699(90)90068-k. [DOI] [PubMed] [Google Scholar]
  3. Caruso A., Bonfanti C., Colombrita D., De Francesco M., De Rango C., Foresti I., Gargiulo F., Gonzales R., Gribaudo G., Landolfo S. Natural antibodies to IFN-gamma in man and their increase during viral infection. J Immunol. 1990 Jan 15;144(2):685–690. [PubMed] [Google Scholar]
  4. Fomsgaard A., Svenson M., Bendtzen K. Auto-antibodies to tumour necrosis factor alpha in healthy humans and patients with inflammatory diseases and gram-negative bacterial infections. Scand J Immunol. 1989 Aug;30(2):219–223. doi: 10.1111/j.1365-3083.1989.tb01204.x. [DOI] [PubMed] [Google Scholar]
  5. Hansen M. B., Nielsen S. E., Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 1989 May 12;119(2):203–210. doi: 10.1016/0022-1759(89)90397-9. [DOI] [PubMed] [Google Scholar]
  6. Hansen M. B., Ross C., Berg K. A sensitive antiviral neutralization bioassay for measuring antibodies to interferons. J Immunol Methods. 1990 Mar 9;127(2):241–248. doi: 10.1016/0022-1759(90)90074-6. [DOI] [PubMed] [Google Scholar]
  7. Hansen M. B., Svenson M., Bendtzen K. Human anti-interleukin 1 alpha antibodies. Immunol Lett. 1991 Sep;30(1):133–139. doi: 10.1016/0165-2478(91)90102-g. [DOI] [PubMed] [Google Scholar]
  8. Hansen M. B., Svenson M., Diamant M., Bendtzen K. Anti-interleukin-6 antibodies in normal human serum. Scand J Immunol. 1991 Jun;33(6):777–781. doi: 10.1111/j.1365-3083.1991.tb02552.x. [DOI] [PubMed] [Google Scholar]
  9. Ikeda Y., Toda G., Hashimoto N., Umeda N., Miyake K., Yamanaka M., Kurokowa K. Naturally occurring anti-interferon-alpha 2a antibodies in patients with acute viral hepatitis. Clin Exp Immunol. 1991 Jul;85(1):80–84. doi: 10.1111/j.1365-2249.1991.tb05686.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jeffes E. W., 3rd, Ininns E. K., Schmitz K. L., Yamamoto R. S., Dett C. A., Granger G. A. The presence of antibodies to lymphotoxin and tumor necrosis factor in normal serum. Arthritis Rheum. 1989 Sep;32(9):1148–1152. doi: 10.1002/anr.1780320914. [DOI] [PubMed] [Google Scholar]
  11. Mae N., Liberato D. J., Chizzonite R., Satoh H. Identification of high-affinity anti-IL-1 alpha autoantibodies in normal human serum as an interfering substance in a sensitive enzyme-linked immunosorbent assay for IL-1 alpha. Lymphokine Cytokine Res. 1991 Apr;10(1-2):61–68. [PubMed] [Google Scholar]
  12. Mogensen K. E., Uzé G. Radioiodination of human alpha interferons by the chloramine T method. Methods Enzymol. 1986;119:267–276. doi: 10.1016/0076-6879(86)19039-2. [DOI] [PubMed] [Google Scholar]
  13. Ross C., Hansen M. B., Schyberg T., Berg K. Autoantibodies to crude human leucocyte interferon (IFN), native human IFN, recombinant human IFN-alpha 2b and human IFN-gamma in healthy blood donors. Clin Exp Immunol. 1990 Oct;82(1):57–62. doi: 10.1111/j.1365-2249.1990.tb05403.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Suzuki H., Ayabe T., Kamimura J., Kashiwagi H. Anti-IL-1 alpha autoantibodies in patients with rheumatic diseases and in healthy subjects. Clin Exp Immunol. 1991 Sep;85(3):407–412. doi: 10.1111/j.1365-2249.1991.tb05740.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Suzuki H., Kamimura J., Ayabe T., Kashiwagi H. Demonstration of neutralizing autoantibodies against IL-1 alpha in sera from patients with rheumatoid arthritis. J Immunol. 1990 Oct 1;145(7):2140–2146. [PubMed] [Google Scholar]
  16. Svenson M., Bagge Hansen M., Bendtzen K. Distribution and characterization of autoantibodies to interleukin 1 alpha in normal human sera. Scand J Immunol. 1990 Dec;32(6):695–701. doi: 10.1111/j.1365-3083.1990.tb03212.x. [DOI] [PubMed] [Google Scholar]
  17. Svenson M., Kayser L., Hansen M. B., Rasmussen A. K., Bendtzen K. Interleukin-1 receptors on human thyroid cells and on the rat thyroid cell line FRTL-5. Cytokine. 1991 Mar;3(2):125–130. doi: 10.1016/1043-4666(91)90032-9. [DOI] [PubMed] [Google Scholar]
  18. Svenson M., Poulsen L. K., Fomsgaard A., Bendtzen K. IgG autoantibodies against interleukin 1 alpha in sera of normal individuals. Scand J Immunol. 1989 Apr;29(4):489–492. doi: 10.1111/j.1365-3083.1989.tb01149.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES