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. 1992 Dec;90(3):453–458. doi: 10.1111/j.1365-2249.1992.tb05867.x

Glomerular immune deposits in murine lupus models may contain histones.

T Schmiedeke 1, F Stoeckl 1, S Muller 1, Y Sugisaki 1, S Batsford 1, R Woitas 1, A Vogt 1
PMCID: PMC1554586  PMID: 1458682

Abstract

Two types of lupus mice, NZB/NZW F1 female hybrids and mice with graft-versus-host disease (GVHD), were studied. Histones H3 and H2A were detected by immunofluorescence in glomeruli of 22/22 proteinuric GVHD and 8/12 proteinuric NZB/W F1 female mice; in non-proteinuric animals, 3/5 GVHD and 2/27 NZB/W F1 female were positive. Using antibodies to histone peptides it was shown that mainly the N-terminal regions of histones H3 and H2A were exposed in glomerular deposits. Western blot analysis revealed antibodies to histone subfractions in sera of 33/34 lupus mice that developed proteinuria. This study provides evidence that histones are involved in the pathogenesis of lupus nephritis.

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Selected References

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