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. 1992 Nov;90(2):271–274. doi: 10.1111/j.1365-2249.1992.tb07941.x

Circulating antibodies to heat-shock protein 60 in Crohn's disease and ulcerative colitis.

T R Stevens 1, V R Winrow 1, D R Blake 1, D S Rampton 1
PMCID: PMC1554622  PMID: 1424286

Abstract

Heat-shock proteins (HSPs) are highly conserved immunogenic intracellular molecules that are induced by inflammatory mediators and may induce autoimmune phenomena in vivo. We have recently demonstrated the increased expression of HSP-60 in the colonocytes of patients with ulcerative colitis. To study further the role of HSP-60 in inflammatory bowel disease, we have now measured antibodies to recombinant mycobacterial HSP-65 (a member of the HSP-60 family) in patients with Crohn's disease, ulcerative colitis, healthy volunteers and, as disease controls, patients with confirmed bacterial diarrhoea. In comparison with healthy controls (n = 20; median level of 89 ELISA units; range 24-292), serum IgA HSP-60 antibodies were elevated in Crohn's disease (n = 21; 157; 57-364; P < 0.05) and active ulcerative colitis (n = 16; 188; 58-373; P < 0.01) but not bacterial diarrhoea (n = 10; 106; 51-285). Increased IgA HSP-60 antibody levels in patients with inflammatory bowel disease may occur as the result of HSP release from injured gut epithelium; alternatively, increased intestinal permeability could facilitate mucosal access of luminal antigens and the generation of cross-reactive anti-bacterial HSP antibodies.

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Selected References

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  1. Burnham W. R., Lennard-Jones J. E., Stanford J. L., Bird R. G. Mycobacteria as a possible cause of inflammatory bowel disease. Lancet. 1978 Sep 30;2(8092 Pt 1):693–696. doi: 10.1016/s0140-6736(78)92699-5. [DOI] [PubMed] [Google Scholar]
  2. Cho S. N., Brennan P. J., Yoshimura H. H., Korelitz B. I., Graham D. Y. Mycobacterial aetiology of Crohn's disease: serologic study using common mycobacterial antigens and a species-specific glycolipid antigen from Mycobacterium paratuberculosis. Gut. 1986 Nov;27(11):1353–1356. doi: 10.1136/gut.27.11.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen I. R., Holoshitz J., van Eden W., Frenkel A. T lymphocyte clones illuminate pathogenesis and affect therapy of experimental arthritis. Arthritis Rheum. 1985 Aug;28(8):841–845. doi: 10.1002/art.1780280802. [DOI] [PubMed] [Google Scholar]
  4. Cooper R., Fraser S. M., Sturrock R. D., Gemmell C. G. Raised titres of anti-klebsiella IgA in ankylosing spondylitis, rheumatoid arthritis, and inflammatory bowel disease. Br Med J (Clin Res Ed) 1988 May 21;296(6634):1432–1434. doi: 10.1136/bmj.296.6634.1432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellis R. J. The molecular chaperone concept. Semin Cell Biol. 1990 Feb;1(1):1–9. [PubMed] [Google Scholar]
  6. Hollander D., Vadheim C. M., Brettholz E., Petersen G. M., Delahunty T., Rotter J. I. Increased intestinal permeability in patients with Crohn's disease and their relatives. A possible etiologic factor. Ann Intern Med. 1986 Dec;105(6):883–885. doi: 10.7326/0003-4819-105-6-883. [DOI] [PubMed] [Google Scholar]
  7. Holoshitz J., Klajman A., Drucker I., Lapidot Z., Yaretzky A., Frenkel A., van Eden W., Cohen I. R. T lymphocytes of rheumatoid arthritis patients show augmented reactivity to a fraction of mycobacteria cross-reactive with cartilage. Lancet. 1986 Aug 9;2(8502):305–309. doi: 10.1016/s0140-6736(86)90003-6. [DOI] [PubMed] [Google Scholar]
  8. Jarjour W. N., Jeffries B. D., Davis J. S., 4th, Welch W. J., Mimura T., Winfield J. B. Autoantibodies to human stress proteins. A survey of various rheumatic and other inflammatory diseases. Arthritis Rheum. 1991 Sep;34(9):1133–1138. doi: 10.1002/art.1780340909. [DOI] [PubMed] [Google Scholar]
  9. Kaufmann S. H. Heat shock proteins and the immune response. Immunol Today. 1990 Apr;11(4):129–136. doi: 10.1016/0167-5699(90)90050-j. [DOI] [PubMed] [Google Scholar]
  10. Kobayashi K., Brown W. R., Brennan P. J., Blaser M. J. Serum antibodies to mycobacterial antigens in active Crohn's disease. Gastroenterology. 1988 Jun;94(6):1404–1411. doi: 10.1016/0016-5085(88)90679-8. [DOI] [PubMed] [Google Scholar]
  11. Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
  12. Markesich D. C., Sawai E. T., Butel J. S., Graham D. Y. Investigations on etiology of Crohn's disease. Humoral immune response to stress (heat shock) proteins. Dig Dis Sci. 1991 Apr;36(4):454–460. doi: 10.1007/BF01298874. [DOI] [PubMed] [Google Scholar]
  13. Pelham H. R. Hsp70 accelerates the recovery of nucleolar morphology after heat shock. EMBO J. 1984 Dec 20;3(13):3095–3100. doi: 10.1002/j.1460-2075.1984.tb02264.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Res P. C., Schaar C. G., Breedveld F. C., van Eden W., van Embden J. D., Cohen I. R., de Vries R. R. Synovial fluid T cell reactivity against 65 kD heat shock protein of mycobacteria in early chronic arthritis. Lancet. 1988 Aug 27;2(8609):478–480. doi: 10.1016/s0140-6736(88)90123-7. [DOI] [PubMed] [Google Scholar]
  15. Stevens T. R., James J. P., Simmonds N. J., McCarthy D. A., Laurenson I. F., Maddison P. J., Rampton D. S. Circulating von Willebrand factor in inflammatory bowel disease. Gut. 1992 Apr;33(4):502–506. doi: 10.1136/gut.33.4.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Teahon K., Smethurst P., Levi A. J., Menzies I. S., Bjarnason I. Intestinal permeability in patients with Crohn's disease and their first degree relatives. Gut. 1992 Mar;33(3):320–323. doi: 10.1136/gut.33.3.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tsoulfa G., Rook G. A., Van-Embden J. D., Young D. B., Mehlert A., Isenberg D. A., Hay F. C., Lydyard P. M. Raised serum IgG and IgA antibodies to mycobacterial antigens in rheumatoid arthritis. Ann Rheum Dis. 1989 Feb;48(2):118–123. doi: 10.1136/ard.48.2.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winfield J. B. Stress proteins, arthritis, and autoimmunity. Arthritis Rheum. 1989 Dec;32(12):1497–1504. doi: 10.1002/anr.1780321202. [DOI] [PubMed] [Google Scholar]
  19. Winrow V. R., McLean L., Morris C. J., Blake D. R. The heat shock protein response and its role in inflammatory disease. Ann Rheum Dis. 1990 Feb;49(2):128–132. doi: 10.1136/ard.49.2.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Graeff-Meeder E. R., Voorhorst M., van Eden W., Schuurman H. J., Huber J., Barkley D., Maini R. N., Kuis W., Rijkers G. T., Zegers B. J. Antibodies to the mycobacterial 65-kd heat-shock protein are reactive with synovial tissue of adjuvant arthritic rats and patients with rheumatoid arthritis and osteoarthritis. Am J Pathol. 1990 Nov;137(5):1013–1017. [PMC free article] [PubMed] [Google Scholar]

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