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. 1993 Jan;91(1):135–140. doi: 10.1111/j.1365-2249.1993.tb03368.x

Effect of chlorpromazine on kinetics of injected monoclonal antibody in MoAb-induced glomerular injury.

N Takashima 1, H Kawachi 1, T Oite 1, S Nishi 1, M Arakawa 1, F Shimizu 1
PMCID: PMC1554648  PMID: 8419074

Abstract

The effect of chlorpromazine, one of several calmodulin antagonists that inhibit cytoskeletal movement, on the local kinetics of injected proteinuria-inducing MoAb 5-1-6 was examined to test the hypothesis that proteinuria is inhibited if the antigen recognized by MoAb 5-1-6 or injected MoAb remains on the surface of epithelial foot processes. MoAb 5-1-6 was injected into both chlorpromazine-treated (5 mg/100 g body weight) and untreated rats. As a positive control for the chlorpromazine treatment, anti-Fx1A serum was also injected into other chlorpromazine-treated and untreated rats. Chlorpromazine inhibited neither the change in localization of injected MoAb 5-1-6 nor proteinuria, although it showed an inhibitory effect on redistribution of immune complex and the fixation of complement in passive Heymann glomerulonephritis induced by injection of anti-Fx1A serum. We conclude that the kinetics of bound MoAb 5-1-6 are regulated by a system different from that operating in passive Heymann glomerulonephritis.

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Selected References

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