Abstract
After 3-4 weeks culture of human bone marrow cells in medium supplemented with IL-3, macrophage- (M-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF), the firmly adherent cells exhibited the morphologic features of mononuclear phagocytes and were strongly esterase-positive. Flow cytometric analysis revealed a rather homogeneous cell population with marked autofluorescence; the large majority of the cells expressed CD14, CD11a, b, and c, Fc receptors for IgG, Fc gamma RI, II, and III, and HLA class II molecules. Interferon-gamma (IFN-gamma), bacteria, and bacterial products modulated expression of some of the surface markers, induced and/or enhanced respiratory burst, phagocytic activity, secretion of tumour necrosis factor, and tumouricidal activity; in contrast, these cells were not able to generate reactive nitrogen intermediates.
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Selected References
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- Andreoni C., Moreau I., Rigal D. Long-term culture of human bone marrow. I. characterization of adherent cells in flow cytometry. Exp Hematol. 1990 Jun;18(5):431–437. [PubMed] [Google Scholar]
- Drach J., Gattringer C., Glassl H., Schwarting R., Stein H., Huber H. Simultaneous flow cytometric analysis of surface markers and nuclear Ki-67 antigen in leukemia and lymphoma. Cytometry. 1989 Nov;10(6):743–749. doi: 10.1002/cyto.990100612. [DOI] [PubMed] [Google Scholar]
- Dransfield I., Corcoran D., Partridge L. J., Hogg N., Burton D. R. Comparison of human monocytes isolated by elutriation and adherence suggests that heterogeneity may reflect a continuum of maturation/activation states. Immunology. 1988 Mar;63(3):491–498. [PMC free article] [PubMed] [Google Scholar]
- Gartner S., Kaplan H. S. Long-term culture of human bone marrow cells. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4756–4759. doi: 10.1073/pnas.77.8.4756. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hocking W. G., Golde D. W. Long-term human bone marrow cultures. Blood. 1980 Jul;56(1):118–124. [PubMed] [Google Scholar]
- Hogg N. Human mononuclear phagocyte molecules and the use of monoclonal antibodies in their detection. Clin Exp Immunol. 1987 Sep;69(3):687–694. [PMC free article] [PubMed] [Google Scholar]
- Keller R., Gehri R., Keist R., Huf E., Kayser F. H. The interaction of macrophages and bacteria: a comparative study of the induction of tumoricidal activity and of reactive nitrogen intermediates. Cell Immunol. 1991 Apr 15;134(1):249–256. doi: 10.1016/0008-8749(91)90348-f. [DOI] [PubMed] [Google Scholar]
- Keller R., Gehri R., Keist R. The interaction of macrophages and bacteria: Escherichia coli species, bacterial lipopolysaccharide, and lipid A differ in their ability to induce tumoricidal activity and the secretion of reactive nitrogen intermediates in macrophages. Cell Immunol. 1992 Apr 15;141(1):47–58. doi: 10.1016/0008-8749(92)90126-a. [DOI] [PubMed] [Google Scholar]
- Keller R., Gustafson J. E., Keist R. The macrophage response to bacteria. Modulation of macrophage functional activity by peptidoglycan from Moraxella (Branhamella) catarrhalis. Clin Exp Immunol. 1992 Sep;89(3):384–389. doi: 10.1111/j.1365-2249.1992.tb06967.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keller R., Joller P. W., Keist R. Surface phenotype of rat bone marrow-derived mononuclear phagocytes. Cell Immunol. 1989 Apr 15;120(1):277–285. doi: 10.1016/0008-8749(89)90195-0. [DOI] [PubMed] [Google Scholar]
- Keller R., Keist R., Bazin H., Joller P., Van der Meide P. H. Binding of monomeric immunoglobulins by bone marrow-derived mononuclear phagocytes; its modulation by interferon-gamma. Eur J Immunol. 1990 Sep;20(9):2137–2140. doi: 10.1002/eji.1830200937. [DOI] [PubMed] [Google Scholar]
- Keller R., Keist R. Tumor-promoting phorbol esters induce macrophage differentiation from bone marrow precursors. Exp Cell Biol. 1982;50(5):255–265. doi: 10.1159/000163154. [DOI] [PubMed] [Google Scholar]
- Keller R., Keist R., Van der Meide P. H., Groscurth P., Aguet M., Leist T. P. Induction, maintenance, and reinduction of tumoricidal activity in bone marrow-derived mononuclear phagocytes by Corynebacterium parvum. Evidence for the involvement of a T cell- and interferon-gamma-independent pathway of macrophage activation. J Immunol. 1987 Apr 1;138(7):2366–2371. [PubMed] [Google Scholar]
- Keller R., Keist R., Wechsler A., Leist T. P., van der Meide P. H. Mechanisms of macrophage-mediated tumor cell killing: a comparative analysis of the roles of reactive nitrogen intermediates and tumor necrosis factor. Int J Cancer. 1990 Oct 15;46(4):682–686. doi: 10.1002/ijc.2910460422. [DOI] [PubMed] [Google Scholar]
- Keller R., Keist R., van der Meide P. H. Modulation of tumoricidal activity, induced in bone-marrow-derived mononuclear phagocytes by interferon gamma or Corynebacterium parvum, by interferon beta, tumor necrosis factor, prostaglandin E2, and transforming growth factor beta. Int J Cancer. 1991 Nov 11;49(5):796–800. doi: 10.1002/ijc.2910490526. [DOI] [PubMed] [Google Scholar]
- Klostergaard J., Leroux M. E. L-arginine independent macrophage tumor cytotoxicity. Biochem Biophys Res Commun. 1989 Dec 29;165(3):1262–1266. doi: 10.1016/0006-291x(89)92738-1. [DOI] [PubMed] [Google Scholar]
- Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. doi: 10.1016/0022-1759(83)90303-4. [DOI] [PubMed] [Google Scholar]
- Philip R. Cytolysis of tumor necrosis factor (TNF)-resistant tumor targets. Differential cytotoxicity of monocytes activated by the interferons, IL-2, and TNF. J Immunol. 1988 Feb 15;140(4):1345–1349. [PubMed] [Google Scholar]
- Shibata T., Inoue S. Mature T cells are part of adherent cells in human long-term bone marrow cultures. Exp Hematol. 1986 Mar;14(3):234–240. [PubMed] [Google Scholar]
- Stanley E. R., Heard P. M. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. J Biol Chem. 1977 Jun 25;252(12):4305–4312. [PubMed] [Google Scholar]
- Strobel E. S., Gay R. E., Greenberg P. L. Characterization of the in vitro stromal microenvironment of human bone marrow. Int J Cell Cloning. 1986 Sep;4(5):341–356. doi: 10.1002/stem.5530040506. [DOI] [PubMed] [Google Scholar]
- Witsell A. L., Schook L. B. Macrophage heterogeneity occurs through a developmental mechanism. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1963–1967. doi: 10.1073/pnas.88.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]