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. 1993 Mar;91(3):437–441. doi: 10.1111/j.1365-2249.1993.tb05921.x

Calreticulin synthetic peptide analogues: anti-peptide antibodies in autoimmune rheumatic diseases.

J G Routsias 1, A G Tzioufas 1, M Sakarellos-Daitsiotis 1, C Sakarellos 1, H M Moutsopoulos 1
PMCID: PMC1554729  PMID: 7680295

Abstract

Autoantibodies in sera from patients with systemic lupus erythematosus (SLE) and onchocerciasis recognize calreticulin (CaR), a calcium-binding protein, as antigen. In this study we present the immunological properties of two synthetic peptides prepared to correspond to the 1-24 and 7-24 amino acid sequence of CaR. In contrast to information previously reported for the recombinant protein, the CaR-peptide analogues appeared immunoreactive to anti-Ro/SSA autoimmune sera. Human sera from patients with SLE, Sjögren's syndrome (SS), rheumatoid arthritis (RA), as well as mixed connective tissue disease (MCTD), demonstrated a positive autoimmune response (binding of antibodies), to the CaR-peptide analogues. These findings suggest that anti-calreticulin autoantibodies are not restricted to any disease specificity.

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Selected References

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