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. 1993 Jun;92(3):397–403. doi: 10.1111/j.1365-2249.1993.tb03411.x

Expression of various MHC class II molecules and of intracellular adhesion molecule-1 (ICAM-1) on focal clusters of dendritic cells in iodine deficiency goitres

P Dimal, M Wilders-Truschnig, P Mooij, G Leb, O Eber, W Langsteger, J Hebenstreit, A Beham, C Stiegler, G Dohr, H A Drexhage
PMCID: PMC1554788  PMID: 8099855

Abstract

Thyroid sections from 18 consecutive euthyroid patients undergoing surgery for iodine deficiency goitre were investigated by means of immunohistochemistry and immunofluorescence, evaluating the expression of MHC class II antigens (HLA-DR, -DP, -DQ, and RFD1) and intercellular adhesion molecule-1 on the formerly described clusters of dendritic cells, as well as on thyrocytes. Eleven of 18 iodine deficiency goitres contained clusters of dendritic cells. These clusters appeared to express only HLA-DR in two cases; in nine of 12 cases they showed a differential expression of class II molecules in the following frequency: HLA-DR>DQ and/or -DP>RFD1. These dendritic cells also were ICAM-1+. In four of 18 iodine deficiency goitres, thyroid epithelial cells showed MHC class II expression in several combinations, but were ICAM-1-. In normal thyroids and in nodular goitres from inhabitants of the endemic area not having an actual iodine deficiency, only sparse clusters of dendritic cells were found; these cells were only HLA-DR+. Follicle lining cells were negative for the MHC class II molecules. In normal thyroids from an area with sufficient iodine supply, no clusters of dendritic cells were seen. The few dendritic cells observed were lying isolated in the interstitium and only positive for HLA-DR and ICAM-1; epithelial cells were negative for the studied markers. These data show clusters of dendritic cells in thyroids of inhabitants of an endemic area. When goitre is accompanied by iodine deficiency at the moment of operation, there appears to be activation of these dendritic cells and of thyroid epithelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aichinger G., Fill H., Wick G. In situ immune complexes, lymphocyte subpopulations, and HLA-DR-positive epithelial cells in Hashimoto thyroiditis. Lab Invest. 1985 Feb;52(2):132–140. [PubMed] [Google Scholar]
  2. Allaerts W., Denef C. Regulatory activity and topological distribution of folliculo-stellate cells in rat anterior pituitary cell aggregates. Neuroendocrinology. 1989 Apr;49(4):409–418. doi: 10.1159/000125146. [DOI] [PubMed] [Google Scholar]
  3. Baes M., Allaerts W., Denef C. Evidence for functional communication between folliculo-stellate cells and hormone-secreting cells in perifused anterior pituitary cell aggregates. Endocrinology. 1987 Feb;120(2):685–691. doi: 10.1210/endo-120-2-685. [DOI] [PubMed] [Google Scholar]
  4. Bagnasco M., Caretto A., Olive D., Pedini B., Canonica G. W., Betterle C. Expression of intercellular adhesion molecule-1 (ICAM-1) on thyroid epithelial cells in Hashimoto's thyroiditis but not in Graves' disease or papillary thyroid cancer. Clin Exp Immunol. 1991 Feb;83(2):309–313. doi: 10.1111/j.1365-2249.1991.tb05633.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bottazzo G. F., Pujol-Borrell R., Hanafusa T., Feldmann M. Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity. Lancet. 1983 Nov 12;2(8359):1115–1119. doi: 10.1016/s0140-6736(83)90629-3. [DOI] [PubMed] [Google Scholar]
  6. Brooks C. F., Moore M. Differential MHC class II expression on human peripheral blood monocytes and dendritic cells. Immunology. 1988 Feb;63(2):303–311. [PMC free article] [PubMed] [Google Scholar]
  7. Fenzi G. F., Giani C., Ceccarelli P., Bartalena L., Macchia E., Aghini-Lombardi F., Vitti P., Lari R., Ceccarelli C., Baschieri L. Role of autoimmune and familial factors in goiter prevalence. Studies performed in a moderately endemic area. J Endocrinol Invest. 1986 Apr;9(2):161–164. doi: 10.1007/BF03348088. [DOI] [PubMed] [Google Scholar]
  8. Hamilton F., Black M., Farquharson M. A., Stewart C., Foulis A. K. Spatial correlation between thyroid epithelial cells expressing class II MHC molecules and interferon-gamma-containing lymphocytes in human thyroid autoimmune disease. Clin Exp Immunol. 1991 Jan;83(1):64–68. doi: 10.1111/j.1365-2249.1991.tb05589.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanafusa T., Pujol-Borrell R., Chiovato L., Russell R. C., Doniach D., Bottazzo G. F. Aberrant expression of HLA-DR antigen on thyrocytes in Graves' disease: relevance for autoimmunity. Lancet. 1983 Nov 12;2(8359):1111–1115. doi: 10.1016/s0140-6736(83)90628-1. [DOI] [PubMed] [Google Scholar]
  10. Iwatani Y., Amino N., Miyai K. Fail-safe mechanism against autoimmunity. Lancet. 1989 May 20;1(8647):1141–1141. doi: 10.1016/s0140-6736(89)92420-3. [DOI] [PubMed] [Google Scholar]
  11. Iwatani Y., Amino N., Miyai K. Peripheral self-tolerance and autoimmunity: the protective role of expression of class II major histocompatibility antigens on non-lymphoid cells. Biomed Pharmacother. 1989;43(8):593–605. doi: 10.1016/0753-3322(89)90038-3. [DOI] [PubMed] [Google Scholar]
  12. Iwatani Y., Iitaka M., Row V. V., Volpé R. Effect of HLA-DR positive thyrocytes on in vitro thyroid autoantibody production. Clin Invest Med. 1988 Aug;11(4):279–285. [PubMed] [Google Scholar]
  13. Kabel P. J., Voorbij H. A., De Haan M., van der Gaag R. D., Drexhage H. A. Intrathyroidal dendritic cells. J Clin Endocrinol Metab. 1988 Jan;66(1):199–207. doi: 10.1210/jcem-66-1-199. [DOI] [PubMed] [Google Scholar]
  14. Keizer G. D., Te Velde A. A., Schwarting R., Figdor C. G., De Vries J. E. Role of p150,95 in adhesion, migration, chemotaxis and phagocytosis of human monocytes. Eur J Immunol. 1987 Sep;17(9):1317–1322. doi: 10.1002/eji.1830170915. [DOI] [PubMed] [Google Scholar]
  15. Lucas-Martin A., Foz-Sala M., Todd I., Bottazzo G. F., Pujol-Borrell R. Occurrence of thyrocyte HLA class II expression in a wide variety of thyroid diseases: relationship with lymphocytic infiltration and thyroid autoantibodies. J Clin Endocrinol Metab. 1988 Feb;66(2):367–375. doi: 10.1210/jcem-66-2-367. [DOI] [PubMed] [Google Scholar]
  16. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  17. Schatz H., Pschierer-Berg K., Nickel J. A., Bär R., Müller F., Bretzel R. G., Müller H., Stracke H. Assay for thyroid growth stimulating immunoglobulins: stimulation of [3H]thymidine incorporation into isolated thyroid follicles by TSH, EGF, and immunoglobulins from goitrous patients in an iodine-deficient region. Acta Endocrinol (Copenh) 1986 Aug;112(4):523–530. doi: 10.1530/acta.0.1120523. [DOI] [PubMed] [Google Scholar]
  18. Silberberg-Sinakin I., Thorbecke G. J., Baer R. L., Rosenthal S. A., Berezowsky V. Antigen-bearing langerhans cells in skin, dermal lymphatics and in lymph nodes. Cell Immunol. 1976 Aug;25(2):137–151. doi: 10.1016/0008-8749(76)90105-2. [DOI] [PubMed] [Google Scholar]
  19. Spiteri M. A., Clarke S. W., Poulter L. W. Phenotypic and functional changes in alveolar macrophages contribute to the pathogenesis of pulmonary sarcoidosis. Clin Exp Immunol. 1988 Dec;74(3):359–364. [PMC free article] [PubMed] [Google Scholar]
  20. Todd I., Pujol-Borrell R., Hammond L. J., Bottazzo G. F., Feldmann M. Interferon-gamma induces HLA-DR expression by thyroid epithelium. Clin Exp Immunol. 1985 Aug;61(2):265–273. [PMC free article] [PubMed] [Google Scholar]
  21. Valente W. A., Vitti P., Rotella C. M., Vaughan M. M., Aloj S. M., Grollman E. F., Ambesi-Impiombato F. S., Kohn L. D. Antibodies that promote thyroid growth. A distinct population of thyroid-stimulating autoantibodies. N Engl J Med. 1983 Oct 27;309(17):1028–1034. doi: 10.1056/NEJM198310273091705. [DOI] [PubMed] [Google Scholar]
  22. Vankelecom H., Carmeliet P., Van Damme J., Billiau A., Denef C. Production of interleukin-6 by folliculo-stellate cells of the anterior pituitary gland in a histiotypic cell aggregate culture system. Neuroendocrinology. 1989 Jan;49(1):102–106. doi: 10.1159/000125097. [DOI] [PubMed] [Google Scholar]
  23. Watson A. J., DeMars R., Trowbridge I. S., Bach F. H. Detection of a novel human class II HLA antigen. 1983 Jul 28-Aug 3Nature. 304(5924):358–361. doi: 10.1038/304358a0. [DOI] [PubMed] [Google Scholar]
  24. Weetman A. P., Cohen S., Makgoba M. W., Borysiewicz L. K. Expression of an intercellular adhesion molecule, ICAM-1, by human thyroid cells. J Endocrinol. 1989 Jul;122(1):185–191. doi: 10.1677/joe.0.1220185. [DOI] [PubMed] [Google Scholar]
  25. Wilders-Truschnig M. M., Drexhage H. A., Leb G., Eber O., Brezinschek H. P., Dohr G., Lanzer G., Krejs G. J. Chromatographically purified immunoglobulin G of endemic and sporadic goiter patients stimulates FRTL5 cell growth in a mitotic arrest assay. J Clin Endocrinol Metab. 1990 Feb;70(2):444–452. doi: 10.1210/jcem-70-2-444. [DOI] [PubMed] [Google Scholar]
  26. Wilders-Truschnig M. M., Kabel P. J., Drexhage H. A., Beham A., Leb G., Eber O., Hebenstreit J., Loidolt D., Dohr G., Lanzer G. Intrathyroidal dendritic cells, epitheloid cells, and giant cells in iodine deficient goiter. Am J Pathol. 1989 Jul;135(1):219–225. [PMC free article] [PubMed] [Google Scholar]
  27. Zakarija M., McKenzie J. M. Do thyroid growth-promoting immunoglobulins exist? J Clin Endocrinol Metab. 1990 Feb;70(2):308–310. doi: 10.1210/jcem-70-2-308. [DOI] [PubMed] [Google Scholar]
  28. Ziegler A., Uchańska-Ziegler B., Zeuthen J., Wernet P. HLA antigen expression at the single cell level on a K562 X B cell hybrid: an analysis with monoclonal antibodies using bacterial binding assays. Somatic Cell Genet. 1982 Nov;8(6):775–789. doi: 10.1007/BF01543018. [DOI] [PubMed] [Google Scholar]
  29. von Overbeck J., Stähli C., Gudat F., Carmann H., Lautenschlager C., Dürmüller U., Takacs B., Miggiano V., Staehelin T., Heitz P. U. Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5). Virchows Arch A Pathol Anat Histopathol. 1985;407(1):1–12. doi: 10.1007/BF00701324. [DOI] [PubMed] [Google Scholar]

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