Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1993 May;92(2):245–250. doi: 10.1111/j.1365-2249.1993.tb03387.x

Expression of CD5 and CD72 on T and B cell subsets in rheumatoid arthritis and Sjögren's syndrome.

C Jamin 1, A Lamour 1, Y L Pennec 1, M Hirn 1, P Le Goff 1, P Youinou 1
PMCID: PMC1554816  PMID: 7683586

Abstract

A minority of B cells express the CD5 marker, which is found on virtually all T cells, and CD72 has been defined as the CD5 ligand on the B cell membrane. The mean fluorescence intensity (MFI) of the CD5 molecules was shown to be higher on CD4+CD29+ than CD4+CD45RA+ in peripheral blood (PB) and synovial fluid (SF) of rheumatoid arthritis (RA) patients (P < 0.0001 and < 0.001), and PB of Sjögren's syndrome (SS) patients and normal controls (P < 0.02 and < 0.03). This MFI declined once the CD4 expressed HLA-DR in PB of SS patients (P < 0.004) and normal controls (P < 0.02) or CD25 in PB of RA (P < 0.004) and SS patients (P < 0.0004). There was a correlation between the CD5 MFI on CD4+CD45RA+ and CD4+CD29+ in RA (P < 0.001) as well as SS (P < 0.0007) PB. The CD72 MFI was impressively higher on CD5+ than CD5- B cells in PB and SF of RA patients (P < 0.0001 and P < 0.005) and PB of SS patients (P < 0.005) and normal controls (P < 0.005). Our data suggest that, in association with CD4CD29, CD5 is involved in CD5+B/CD5+ B cell interactions in non-organ-specific autoimmune diseases.

Full text

PDF
245

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson T. C., 3rd, Fox R. I., Frisman D. M., Howell F. V. Immunohistologic analysis of lymphoid infiltrates in primary Sjogren's syndrome using monoclonal antibodies. J Immunol. 1983 Jan;130(1):203–208. [PubMed] [Google Scholar]
  2. Akbar A. N., Terry L., Timms A., Beverley P. C., Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1;140(7):2171–2178. [PubMed] [Google Scholar]
  3. Arnett F. C., Edworthy S. M., Bloch D. A., McShane D. J., Fries J. F., Cooper N. S., Healey L. A., Kaplan S. R., Liang M. H., Luthra H. S. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988 Mar;31(3):315–324. doi: 10.1002/art.1780310302. [DOI] [PubMed] [Google Scholar]
  4. Caligaris-Cappio F., Gobbi M., Bofill M., Janossy G. Infrequent normal B lymphocytes express features of B-chronic lymphocytic leukemia. J Exp Med. 1982 Feb 1;155(2):623–628. doi: 10.1084/jem.155.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cush J. J., Lipsky P. E. Phenotypic analysis of synovial tissue and peripheral blood lymphocytes isolated from patients with rheumatoid arthritis. Arthritis Rheum. 1988 Oct;31(10):1230–1238. doi: 10.1002/art.1780311003. [DOI] [PubMed] [Google Scholar]
  6. Dalavanga Y. A., Drosos A. A., Moutsopoulos H. M. Labial salivary gland immunopathology in Sjögren's syndrome. Scand J Rheumatol Suppl. 1986;61:67–70. [PubMed] [Google Scholar]
  7. Dauphinée M., Tovar Z., Talal N. B cells expressing CD5 are increased in Sjögren's syndrome. Arthritis Rheum. 1988 May;31(5):642–647. doi: 10.1002/art.1780310509. [DOI] [PubMed] [Google Scholar]
  8. Emery P., Gentry K. C., Mackay I. R., Muirden K. D., Rowley M. Deficiency of the suppressor inducer subset of T lymphocytes in rheumatoid arthritis. Arthritis Rheum. 1987 Aug;30(8):849–856. doi: 10.1002/art.1780300802. [DOI] [PubMed] [Google Scholar]
  9. Fox R. I., Fong S., Sabharwal N., Carstens S. A., Kung P. C., Vaughan J. H. Synovial fluid lymphocytes differ from peripheral blood lymphocytes in patients with rheumatoid arthritis. J Immunol. 1982 Jan;128(1):351–354. [PubMed] [Google Scholar]
  10. Freedman A. S., Freeman G., Whitman J., Segil J., Daley J., Levine H., Nadler L. M. Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19(5):849–855. doi: 10.1002/eji.1830190511. [DOI] [PubMed] [Google Scholar]
  11. Gogolin-Ewens K., Meeusen E., Lee C. S., Brandon M. Expression of CD5, a lymphocyte surface antigen on the endothelium of blood vessels. Eur J Immunol. 1989 May;19(5):935–938. doi: 10.1002/eji.1830190522. [DOI] [PubMed] [Google Scholar]
  12. Janossy G., Panayi G., Duke O., Bofill M., Poulter L. W., Goldstein G. Rheumatoid arthritis: a disease of T-lymphocyte/macrophage immunoregulation. Lancet. 1981 Oct 17;2(8251):839–842. doi: 10.1016/s0140-6736(81)91107-7. [DOI] [PubMed] [Google Scholar]
  13. Kamal M., Katira A., Gordon J. Stimulation of B lymphocytes via CD72 (human Lyb-2). Eur J Immunol. 1991 Jun;21(6):1419–1424. doi: 10.1002/eji.1830210614. [DOI] [PubMed] [Google Scholar]
  14. Kristensson K., Dohlsten M., Fischer H., Ericsson P. O., Hedlund G., Sjögren H. O., Carlsson R. Phenotypical and functional differentiation of CD4+ CD45RA+ human T cells following polyclonal activation. Scand J Immunol. 1990 Sep;32(3):243–253. doi: 10.1111/j.1365-3083.1990.tb02917.x. [DOI] [PubMed] [Google Scholar]
  15. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981 Feb 1;153(2):310–323. doi: 10.1084/jem.153.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ledbetter J. A., Martin P. J., Spooner C. E., Wofsy D., Tsu T. T., Beatty P. G., Gladstone P. Antibodies to Tp67 and Tp44 augment and sustain proliferative responses of activated T cells. J Immunol. 1985 Oct;135(4):2331–2336. [PubMed] [Google Scholar]
  17. Maurer D., Felzmann T., Holter W., Petera P., Smolen J., Knapp W. Evidence for the presence of activated CD4 T cells with naive phenotype in the peripheral blood of patients with rheumatoid arthritis. Clin Exp Immunol. 1992 Mar;87(3):429–434. doi: 10.1111/j.1365-2249.1992.tb03014.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morimoto C., Letvin N. L., Boyd A. W., Hagan M., Brown H. M., Kornacki M. M., Schlossman S. F. The isolation and characterization of the human helper inducer T cell subset. J Immunol. 1985 Jun;134(6):3762–3769. [PubMed] [Google Scholar]
  19. Morimoto C., Letvin N. L., Distaso J. A., Aldrich W. R., Schlossman S. F. The isolation and characterization of the human suppressor inducer T cell subset. J Immunol. 1985 Mar;134(3):1508–1515. [PubMed] [Google Scholar]
  20. Pirruccello S. J., Collins M., Wilson J. E., McManus B. M. Age-related changes in naive and memory CD4+ T cells in healthy human children. Clin Immunol Immunopathol. 1989 Aug;52(2):341–345. doi: 10.1016/0090-1229(89)90185-2. [DOI] [PubMed] [Google Scholar]
  21. Pitzalis C., Kingsley G., Haskard D., Panayi G. The preferential accumulation of helper-inducer T lymphocytes in inflammatory lesions: evidence for regulation by selective endothelial and homotypic adhesion. Eur J Immunol. 1988 Sep;18(9):1397–1404. doi: 10.1002/eji.1830180915. [DOI] [PubMed] [Google Scholar]
  22. Pitzalis C., Kingsley G., Murphy J., Panayi G. Abnormal distribution of the helper-inducer and suppressor-inducer T-lymphocyte subsets in the rheumatoid joint. Clin Immunol Immunopathol. 1987 Nov;45(2):252–258. doi: 10.1016/0090-1229(87)90040-7. [DOI] [PubMed] [Google Scholar]
  23. Plater-Zyberk C., Maini R. N., Lam K., Kennedy T. D., Janossy G. A rheumatoid arthritis B cell subset expresses a phenotype similar to that in chronic lymphocytic leukemia. Arthritis Rheum. 1985 Sep;28(9):971–976. doi: 10.1002/art.1780280903. [DOI] [PubMed] [Google Scholar]
  24. Sanders M. E., Makgoba M. W., Sharrow S. O., Stephany D., Springer T. A., Young H. A., Shaw S. Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29, and Pgp-1) and have enhanced IFN-gamma production. J Immunol. 1988 Mar 1;140(5):1401–1407. [PubMed] [Google Scholar]
  25. Skopouli F. N., Fox P. C., Galanopoulou V., Atkinson J. C., Jaffe E. S., Moutsopoulos H. M. T cell subpopulations in the labial minor salivary gland histopathologic lesion of Sjögren's syndrome. J Rheumatol. 1991 Feb;18(2):210–214. [PubMed] [Google Scholar]
  26. Sohen S., Romain P. L., Rothstein D. M., Yamane T., Tanaka S., Anderson P., Schlossman S. F., Morimoto C. Phenotypic abnormalities in CD8+ T lymphocyte subsets in patients with rheumatoid arthritis. J Rheumatol. 1991 Nov;18(11):1649–1654. [PubMed] [Google Scholar]
  27. Sohen S., Rothstein D. M., Tallman T., Gaudette D., Schlossman S. F., Morimoto C. The functional heterogeneity of CD8+ cells defined by anti-CD45RA (2H4) and anti-CD29 (4B4) antibodies. Cell Immunol. 1990 Jun;128(1):314–328. doi: 10.1016/0008-8749(90)90028-p. [DOI] [PubMed] [Google Scholar]
  28. Spertini F., Stohl W., Ramesh N., Moody C., Geha R. S. Induction of human T cell proliferation by a monoclonal antibody to CD5. J Immunol. 1991 Jan 1;146(1):47–52. [PubMed] [Google Scholar]
  29. Taga K., Kasahara Y., Yachie A., Miyawaki T., Taniguchi N. Preferential expression of IL-2 receptor subunits on memory populations within CD4+ and CD8+ T cells. Immunology. 1991 Jan;72(1):15–19. [PMC free article] [PubMed] [Google Scholar]
  30. Thomas Y., Glickman E., DeMartino J., Wang J., Goldstein G., Chess L. Biologic functions of the OKT1 T cell surface antigen. I. The T1 molecule is involved in helper function. J Immunol. 1984 Aug;133(2):724–728. [PubMed] [Google Scholar]
  31. Van de Velde H., von Hoegen I., Luo W., Parnes J. R., Thielemans K. The B-cell surface protein CD72/Lyb-2 is the ligand for CD5. Nature. 1991 Jun 20;351(6328):662–665. doi: 10.1038/351662a0. [DOI] [PubMed] [Google Scholar]
  32. Werner-Favre C., Vischer T. L., Wohlwend D., Zubler R. H. Cell surface antigen CD5 is a marker for activated human B cells. Eur J Immunol. 1989 Jul;19(7):1209–1213. doi: 10.1002/eji.1830190709. [DOI] [PubMed] [Google Scholar]
  33. Winrow V. R., Archer J. R., Mitchell D. J. Regarding the ankylosing spondylitis/Klebsiella/HLA-B27 problem: more inconclusive proof! Arthritis Rheum. 1988 Mar;31(3):453–454. doi: 10.1002/art.1780310322. [DOI] [PubMed] [Google Scholar]
  34. Youinou P., Mackenzie L., Katsikis P., Merdrignac G., Isenberg D. A., Tuaillon N., Lamour A., Le Goff P., Jouquan J., Drogou A. The relationship between CD5-expressing B lymphocytes and serologic abnormalities in rheumatoid arthritis patients and their relatives. Arthritis Rheum. 1990 Mar;33(3):339–348. doi: 10.1002/art.1780330306. [DOI] [PubMed] [Google Scholar]
  35. Youinou P., Mackenzie L., le Masson G., Papadopoulos N. M., Jouquan J., Pennec Y. L., Angelidis P., Katsikis P., Moutsopoulos H. M., Lydyard P. M. CD5-expressing B lymphocytes in the blood and salivary glands of patients with primary Sjögren's syndrome. J Autoimmun. 1988 Apr;1(2):185–194. doi: 10.1016/0896-8411(88)90025-x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES