Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1993 Aug;93(2):286–291. doi: 10.1111/j.1365-2249.1993.tb07981.x

Surface expression of adenosine deaminase in mitogen-stimulated lymphocytes.

M Martin 1, J J Centelles 1, J Huguet 1, F Echevarne 1, D Colomer 1, J L Vives-Corrons 1, R Franco 1
PMCID: PMC1554837  PMID: 8348757

Abstract

Adenosine deaminase (ADA) expression on the surface of mitogen-stimulated lymphocytes was studied by flow cytometry. The gate for lymphocytes was located by cell size (forward scatter), cytoplasmic complexity (side scatter) and by expression of the markers CD2, CD4, CD8 and CD19. After mitogenic proliferation two populations appeared, one corresponding to non-stimulated cells, and the other consisting of larger cells which showed relatively high expression of adenosine deaminase on their surface. The increase was similar to that observed for CD71 expression, and paralleled the increase in 3H-thymidine incorporation. There was a correlation between ADA and CD71 expression (r = 0.92 for phytohaemagglutinin (PHA) and 0.97 for pokeweed mitogen (PWM)). These results suggest a role for ecto-adenosine deaminase in lymphocyte proliferation and/or triggering.

Full text

PDF
286

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal R. P., Parks R. E., Jr A possible association between the nucleoside transport system of human erythrocytes and adenosine deaminase. Biochem Pharmacol. 1975 Feb 15;24(4):547–550. doi: 10.1016/0006-2952(75)90146-x. [DOI] [PubMed] [Google Scholar]
  2. Aran J. M., Colomer D., Matutes E., Vives-Corrons J. L., Franco R. Presence of adenosine deaminase on the surface of mononuclear blood cells: immunochemical localization using light and electron microscopy. J Histochem Cytochem. 1991 Aug;39(8):1001–1008. doi: 10.1177/39.8.1856451. [DOI] [PubMed] [Google Scholar]
  3. Baganha M. F., Pêgo A., Lima M. A., Gaspar E. V., Cordeiro A. R. Serum and pleural adenosine deaminase. Correlation with lymphocytic populations. Chest. 1990 Mar;97(3):605–610. doi: 10.1378/chest.97.3.605. [DOI] [PubMed] [Google Scholar]
  4. Barankiewicz J., Dosch H. M., Cohen A. Extracellular nucleotide catabolism in human B and T lymphocytes. The source of adenosine production. J Biol Chem. 1988 May 25;263(15):7094–7098. [PubMed] [Google Scholar]
  5. Barchi J. J., Jr, Marquez V. E., Driscoll J. S., Ford H., Jr, Mitsuya H., Shirasaka T., Aoki S., Kelley J. A. Potential anti-AIDS drugs. Lipophilic, adenosine deaminase-activated prodrugs. J Med Chem. 1991 May;34(5):1647–1655. doi: 10.1021/jm00109a018. [DOI] [PubMed] [Google Scholar]
  6. Bastian J. F., Ruedi J. M., MacPherson G. A., Golembesky H. E., O'Connor R. D., Thompson L. F. Lymphocyte ecto-5'-nucleotidase activity in infancy: increasing activity in peripheral blood B cells precedes their ability to synthesize IgG in vitro. J Immunol. 1984 Apr;132(4):1767–1772. [PubMed] [Google Scholar]
  7. Cao W., Sikorski E. E., Fuchs B. A., Stern M. L., Luster M. I., Munson A. E. The B lymphocyte is the immune cell target for 2',3'-dideoxyadenosine. Toxicol Appl Pharmacol. 1990 Sep 15;105(3):492–502. doi: 10.1016/0041-008x(90)90152-k. [DOI] [PubMed] [Google Scholar]
  8. Edwards N. L., Gelfand E. W., Burk L., Dosch H. M., Fox I. H. Distribution of 5'-nucleotidase in human lymphoid tissues. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3474–3476. doi: 10.1073/pnas.76.7.3474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Franco R., Canela E. I., Bozal J. Heterogeneous localization of some purine enzymes in subcellular fractions of rat brain and cerebellum. Neurochem Res. 1986 Mar;11(3):423–435. doi: 10.1007/BF00965016. [DOI] [PubMed] [Google Scholar]
  10. Grever M. R., Coleman M. S., Balcerzak S. P. Adenosine deaminase and terminal deoxynucleotidyl transferase: biochemical markers in the management of chronic myelogenous leukemia. Cancer Res. 1983 Mar;43(3):1442–1445. [PubMed] [Google Scholar]
  11. HALL J. G. Adenosine deaminase activity in lymphoid cells during antibody production. Aust J Exp Biol Med Sci. 1963 Feb;41:93–97. doi: 10.1038/icb.1963.8. [DOI] [PubMed] [Google Scholar]
  12. Ho A. D., Knauf W., Ganeshaguru K., Hunstein W., Hoffbrand A. V. Purine degradative enzymes in circulating malignant cells of patients with chronic B cell neoplasia. Hematol Oncol. 1987 Jan-Mar;5(1):9–17. doi: 10.1002/hon.2900050103. [DOI] [PubMed] [Google Scholar]
  13. Hovi T., Smyth J. F., Allison A. C., Williams S. C. Role of adenosine deaminase in lymphocyte proliferation. Clin Exp Immunol. 1976 Mar;23(3):395–403. [PMC free article] [PubMed] [Google Scholar]
  14. Ma D. D., Sylwestrowicz T. A., Granger S., Massaia M., Franks R., Janossy G., Hoffbrand A. V. Distribution of terminal deoxynucleotidyl transferase and purine degradative and synthetic enzymes in subpopulations of human thymocytes. J Immunol. 1982 Oct;129(4):1430–1435. [PubMed] [Google Scholar]
  15. Murray J. L., Loftin K. C., Munn C. G., Reuben J. M., Mansell P. W., Hersh E. M. Elevated adenosine deaminase and purine nucleoside phosphorylase activity in peripheral blood null lymphocytes from patients with acquired immune deficiency syndrome. Blood. 1985 Jun;65(6):1318–1324. [PubMed] [Google Scholar]
  16. Murray J. L., Perez-Soler R., Bywaters D., Hersh E. M. Decreased adenosine deaminase (ADA) and 5'nucleotidase (5NT) activity in peripheral blood T cells in Hodgkin disease. Am J Hematol. 1986 Jan;21(1):57–66. doi: 10.1002/ajh.2830210108. [DOI] [PubMed] [Google Scholar]
  17. Schrader J., Berne R. M., Rubio R. Uptake and metabolism of adenosine by human erythrocyte ghosts. Am J Physiol. 1972 Jul;223(1):159–166. doi: 10.1152/ajplegacy.1972.223.1.159. [DOI] [PubMed] [Google Scholar]
  18. Sylwestrowicz T. A., Ma D. D., Murphy P. P., Massaia M., Prentice H. G., Hoffbrand A. V., Greaves M. F. 5'nucleotidase, adenosine deaminase and purine nucleoside phosphorylase activities in acute leukaemia. Leuk Res. 1982;6(4):475–482. doi: 10.1016/0145-2126(82)90004-2. [DOI] [PubMed] [Google Scholar]
  19. Thompson L. F., Ruedi J. M., O'Connor R. D., Bastian J. F. Ecto-5'-nucleotidase expression during human B cell development. An explanation for the heterogeneity in B lymphocyte ecto-5'-nucleotidase activity in patients with hypogammaglobulinemia. J Immunol. 1986 Oct 15;137(8):2496–2500. [PubMed] [Google Scholar]
  20. Thomson L. F., Ruedi J. M., Glass A., Moldenhauer G., Moller P., Low M. G., Klemens M. R., Massaia M., Lucas A. H. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens. 1990 Jan;35(1):9–19. doi: 10.1111/j.1399-0039.1990.tb01750.x. [DOI] [PubMed] [Google Scholar]
  21. Trams E. G., Lauter C. J. Adenosine deaminase of cultured brain cells. Biochem J. 1975 Dec;152(3):681–687. doi: 10.1042/bj1520681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vaitukaitis J. L. Production of antisera with small doses of immunogen: multiple intradermal injections. Methods Enzymol. 1981;73(Pt B):46–52. doi: 10.1016/0076-6879(81)73055-6. [DOI] [PubMed] [Google Scholar]
  23. Vezzoni P., Fiacchino F., Clerici L., Sghirlanzoni A., Cerrato D., Peluchetti D., Lucchini R., Raineri M., Cornelio F. Studies on terminal deoxynucleotidyl transferase and adenosine deaminase in myasthenic thymus. J Neuroimmunol. 1984 Sep-Oct;6(6):427–433. doi: 10.1016/0165-5728(84)90067-5. [DOI] [PubMed] [Google Scholar]
  24. Vezzoni P., Giardini R., Lucchini R., Lombardi L., Vezzoni M. A., Besana C., Clerici L. Adenosine deaminase and terminal deoxynucleotidyl transferase in human lymphomas: an aid to the diagnosis and subclassification of lymphoblastic lymphomas. Am J Hematol. 1985 Jul;19(3):219–227. doi: 10.1002/ajh.2830190303. [DOI] [PubMed] [Google Scholar]
  25. Vives Corrons J. L., Rozman C., Pujades M. A., Colomer D., Perez Vila E., Anegon I., Gallart T., Vives Puiggrós J., Viñolas N., Montserrat E. Combined assay of adenosine deaminase, purine nucleoside phosphorylase, and lactate dehydrogenase in the early clinical evaluation of B-chronic lymphocytic leukemia. Am J Hematol. 1988 Mar;27(3):157–162. doi: 10.1002/ajh.2830270302. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES