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. 1993 Apr;92(1):152–157. doi: 10.1111/j.1365-2249.1993.tb05962.x

Increased spontaneous secretion of rheumatoid factor by intestinal lamina propria mononuclear cells from Crohn's disease but not ulcerative colitis patients.

R P MacDermott 1, S Schreiber 1, G S Nash 1, W J Koopman 1
PMCID: PMC1554872  PMID: 8467559

Abstract

Increased levels of rheumatoid factors (RF) have been observed in the serum of Crohn's disease but not ulcerative colitis patients, and have been proposed to relate to an increased state of intestinal lymphocyte activation. We have therefore examined the spontaneous in vitro secretion of RF by intestinal lamina propria mononuclear cells (MNC) isolated from specimens from control and inflammatory bowel disease (Crohn's disease, ulcerative colitis) patients. Normal intestinal lamina propria MNC spontaneously secrete rheumatoid factors of different isotypes during 14 days of in vitro culture (9.7 ng/ml IgA RF, 11.6 ng/ml IgM RF and 64.6 ng/ml IgA anti-Fc (IgG)). In matched studies intestinal MNC isolated from normal large bowel exhibited significantly greater levels of RF synthesis and secretion in vitro than normal small bowel intestinal MNC. A large increase in spontaneous RF secretion was observed from Crohn's disease intestinal MNC (21.4 ng/ml IgA RF, 21.4 ng/ml IgM RF, and 108.15 ng/ml IgA anti-Fc (IgG)), when compared with normal controls. The amount of RF secreted was dependent on the amount of inflammatory activity of the bowel specimens, from which the MNC were isolated (198.3 ng/ml of IgA anti-Fc(IgG) from involved versus 50.0 ng/ml from matched non-involved tissue). Ulcerative colitis MNC released decreased amounts of RF (7.1 ng/ml IgA RF, 6.2 ng/ml IgM RF, and 42.3 ng/ml IgA anti-Fc(IgG)). These observations using isolated intestinal MNC may explain the findings of RF changes in the sera of inflammatory bowel disease patients. Our observations support the hypothesis of a heightened state of activation in normal intestinal lamina propria MNC, which is further increased in active Crohn's disease. The dissimilarities observed between Crohn's disease and ulcerative colitis may indicate fundamental differences in disease pathophysiology and will lead to further studies exploring intestinal immunoregulatory properties of RF.

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Selected References

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  1. Baklien K., Brandtzaeg P. Comparative mapping of the local distribution of immunoglobulin-containing cells in ulcerative colitis and Crohn's disease of the colon. Clin Exp Immunol. 1975 Nov;22(2):197–209. [PMC free article] [PubMed] [Google Scholar]
  2. Carson D. A., Chen P. P., Fox R. I., Kipps T. J., Jirik F., Goldfien R. D., Silverman G., Radoux V., Fong S. Rheumatoid factor and immune networks. Annu Rev Immunol. 1987;5:109–126. doi: 10.1146/annurev.iy.05.040187.000545. [DOI] [PubMed] [Google Scholar]
  3. Crabtree J. E., Juby L. D., Heatley R. V., Lobo A. J., Bullimore D. W., Axon A. T. Soluble interleukin-2 receptor in Crohn's disease: relation of serum concentrations to disease activity. Gut. 1990 Sep;31(9):1033–1036. doi: 10.1136/gut.31.9.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fais S., Pallone F., Squarcia O., Biancone L., Ricci F., Paoluzi P., Boirivant M. HLA-DR antigens on colonic epithelial cells in inflammatory bowel disease: I. Relation to the state of activation of lamina propria lymphocytes and to the epithelial expression of other surface markers. Clin Exp Immunol. 1987 Jun;68(3):605–612. [PMC free article] [PubMed] [Google Scholar]
  5. Kett K., Rognum T. O., Brandtzaeg P. Mucosal subclass distribution of immunoglobulin G-producing cells is different in ulcerative colitis and Crohn's disease of the colon. Gastroenterology. 1987 Nov;93(5):919–924. doi: 10.1016/0016-5085(87)90552-x. [DOI] [PubMed] [Google Scholar]
  6. Koopman W. J., Schrohenloher R. E. A sensitive radioimmunoassay for quantitation of IgM rheumatoid factor. Arthritis Rheum. 1980 Mar;23(3):302–308. doi: 10.1002/art.1780230306. [DOI] [PubMed] [Google Scholar]
  7. Koopman W. J., Schrohenloher R. E., Czerkinsky C. Differential expression of IgA- and IgM-rheumatoid factors in human disease: evidence for independent regulation of rheumatoid factor isotype production. Adv Exp Med Biol. 1987;216B:1627–1639. [PubMed] [Google Scholar]
  8. MacDermott R. P., Nash G. S., Bertovich M. J., Mohrman R. F., Kodner I. J., Delacroix D. L., Vaerman J. P. Altered patterns of secretion of monomeric IgA and IgA subclass 1 by intestinal mononuclear cells in inflammatory bowel disease. Gastroenterology. 1986 Aug;91(2):379–385. doi: 10.1016/0016-5085(86)90572-x. [DOI] [PubMed] [Google Scholar]
  9. MacDermott R. P., Nash G. S., Bertovich M. J., Seiden M. V., Bragdon M. J., Beale M. G. Alterations of IgM, IgG, and IgA Synthesis and secretion by peripheral blood and intestinal mononuclear cells from patients with ulcerative colitis and Crohn's disease. Gastroenterology. 1981 Nov;81(5):844–852. [PubMed] [Google Scholar]
  10. Mueller C., Knoflach P., Zielinski C. C. T-cell activation in Crohn's disease. Increased levels of soluble interleukin-2 receptor in serum and in supernatants of stimulated peripheral blood mononuclear cells. Gastroenterology. 1990 Mar;98(3):639–646. [PubMed] [Google Scholar]
  11. Nash G. S., Seiden M. V., Beale M. G., MacDermott R. P. A rapid miniaturized solid-phase radioimmunoassay technique for secreted immunoglobulins, employing microtiter plates, antibody bound to polyacrylamide beads, and filter strip harvesting. J Immunol Methods. 1982 Mar 26;49(3):261–269. doi: 10.1016/0022-1759(82)90126-0. [DOI] [PubMed] [Google Scholar]
  12. Pallone F., Fais S., Squarcia O., Biancone L., Pozzilli P., Boirivant M. Activation of peripheral blood and intestinal lamina propria lymphocytes in Crohn's disease. In vivo state of activation and in vitro response to stimulation as defined by the expression of early activation antigens. Gut. 1987 Jun;28(6):745–753. doi: 10.1136/gut.28.6.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pallone F., Matricardi P. M., Squarcia O., Fais S., Le Moli S., Boirivant M., Paoluzi P., D'Amelio R. Raised serum levels of IgM-rheumatoid factor and anti-F(ab')2 autoantibodies in patients with active inflammatory bowel disease. J Clin Lab Immunol. 1986 Apr;19(4):175–180. [PubMed] [Google Scholar]
  14. Peters M. G., Secrist H., Anders K. R., Nash G. S., Rich S. R., MacDermott R. P. Normal human intestinal B lymphocytes. Increased activation compared with peripheral blood. J Clin Invest. 1989 Jun;83(6):1827–1833. doi: 10.1172/JCI114088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosekrans P. C., Meijer C. J., van der Wal A. M., Cornelisse C. J., Lindeman J. Immunoglobulin containing cells in inflammatory bowel disease of the colon: a morphometric and immunohistochemical study. Gut. 1980 Nov;21(11):941–947. doi: 10.1136/gut.21.11.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schreiber S., MacDermott R. P., Raedler A., Pinnau R., Bertovich M. J., Nash G. S. Increased activation of isolated intestinal lamina propria mononuclear cells in inflammatory bowel disease. Gastroenterology. 1991 Oct;101(4):1020–1030. doi: 10.1016/0016-5085(91)90729-5. [DOI] [PubMed] [Google Scholar]
  17. Schreiber S., Raedler A., Conn A. R., Rombeau J. L., MacDermott R. P. Increased in vitro release of soluble interleukin 2 receptor by colonic lamina propria mononuclear cells in inflammatory bowel disease. Gut. 1992 Feb;33(2):236–241. doi: 10.1136/gut.33.2.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scott M. G., Nahm M. H., Macke K., Nash G. S., Bertovich M. J., MacDermott R. P. Spontaneous secretion of IgG subclasses by intestinal mononuclear cells: differences between ulcerative colitis, Crohn's disease, and controls. Clin Exp Immunol. 1986 Oct;66(1):209–215. [PMC free article] [PubMed] [Google Scholar]
  19. Stanley S. L., Jr, Bischoff J. K., Davie J. M. Antigen-induced rheumatoid factors. Protein and carbohydrate antigens induce different rheumatoid factor responses. J Immunol. 1987 Nov 1;139(9):2936–2941. [PubMed] [Google Scholar]

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