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Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1993 Sep;93(3):344–349. doi: 10.1111/j.1365-2249.1993.tb08183.x

Increased CD11/CD18 expression on the peripheral blood leucocytes of patients with HIV disease: relationship to disease severity.

S Palmer 1, A S Hamblin 1
PMCID: PMC1554900  PMID: 8103716

Abstract

In HIV disease increased adhesion between leucocytes themselves and between leucocytes and endothelium may contribute to cell loss and viral spread. Using a novel method for the preparation of blood leucocytes for flow cytometry, we report increased expression of leucocyte adhesion molecules (LeuCAMs) (CD11/CD18) on peripheral blood leucocytes of patients with HIV disease compared with normal controls. Patients were divided into two groups on the basis of CD4 T lymphocyte numbers (those with > 0.5 x 10(9)/l and those with < 0.2 x 10(9)/l), and assessed for p24 antigen expression, viral load and serum tumour necrosis factor (TNF) levels as well as LeuCAM expression. Patients with < 0.2 x 10(9)/lCD4 cells had more p24 antigen and more HIV infectious virus and more serum TNF than those with > 0.5 x 10(9)/l. Whilst the percentages of only monocytes and polymorphs expressing CD11b were significantly increased in patients with the least CD4 cells, the density of LeuCAMs, expressed as mean fluorescence intensity (MFI), was significantly increased on all leucocytes, with the most significant increases being seen on patients with the fewest CD4 T cells. Our findings are consistent with leucocyte activation by a soluble factor, although we could find no correlation between levels of TNF and LeuCAM expression. The increased expression of adhesion molecules on peripheral blood leucocytes could play a role in the cellular extravasation and aggregation seen in HIV disease.

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Selected References

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  1. Arnaout M. A. Structure and function of the leukocyte adhesion molecules CD11/CD18. Blood. 1990 Mar 1;75(5):1037–1050. [PubMed] [Google Scholar]
  2. Barrena M. J., Echaniz P., Garcia-Serrano C., Zubillaga P., Cuadrado E. Differential expression of lymphocyte function-associated antigen (LFA-1) on peripheral blood leucocytes from individuals with Down's syndrome. Clin Exp Immunol. 1992 Apr;88(1):41–44. doi: 10.1111/j.1365-2249.1992.tb03036.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beverley P. C., Callard R. E. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. doi: 10.1002/eji.1830110412. [DOI] [PubMed] [Google Scholar]
  4. Davidson B. L., Kline R. L., Rowland J., Quinn T. C. Surface markers of monocyte function and activation in AIDS. J Infect Dis. 1988 Aug;158(2):483–486. doi: 10.1093/infdis/158.2.483. [DOI] [PubMed] [Google Scholar]
  5. Fauci A. S. AIDS: immunopathogenic mechanisms and research strategies. Clin Res. 1987 Oct;35(6):503–510. [PubMed] [Google Scholar]
  6. Gendelman H. E., Orenstein J. M., Martin M. A., Ferrua C., Mitra R., Phipps T., Wahl L. A., Lane H. C., Fauci A. S., Burke D. S. Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes. J Exp Med. 1988 Apr 1;167(4):1428–1441. doi: 10.1084/jem.167.4.1428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gottlieb M. S., Schroff R., Schanker H. M., Weisman J. D., Fan P. T., Wolf R. A., Saxon A. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency. N Engl J Med. 1981 Dec 10;305(24):1425–1431. doi: 10.1056/NEJM198112103052401. [DOI] [PubMed] [Google Scholar]
  8. Greenfield S. M., Hamblin A., Punchard N. A., Thompson R. P. Expression of adhesion molecules on circulating leucocytes in patients with inflammatory bowel disease. Clin Sci (Lond) 1992 Aug;83(2):221–226. doi: 10.1042/cs0830221. [DOI] [PubMed] [Google Scholar]
  9. Hamblin A., Taylor M., Bernhagen J., Shakoor Z., Mayall S., Noble G., McCarthy D. A method of preparing blood leucocytes for flow cytometry which prevents upregulation of leucocyte integrins. J Immunol Methods. 1992 Feb 5;146(2):219–228. doi: 10.1016/0022-1759(92)90231-h. [DOI] [PubMed] [Google Scholar]
  10. Hildreth J. E., Gotch F. M., Hildreth P. D., McMichael A. J. A human lymphocyte-associated antigen involved in cell-mediated lympholysis. Eur J Immunol. 1983 Mar;13(3):202–208. doi: 10.1002/eji.1830130305. [DOI] [PubMed] [Google Scholar]
  11. Hildreth J. E., Orentas R. J. Involvement of a leukocyte adhesion receptor (LFA-1) in HIV-induced syncytium formation. Science. 1989 Jun 2;244(4908):1075–1078. doi: 10.1126/science.2543075. [DOI] [PubMed] [Google Scholar]
  12. Ho D. D., Rota T. R., Hirsch M. S. Infection of monocyte/macrophages by human T lymphotropic virus type III. J Clin Invest. 1986 May;77(5):1712–1715. doi: 10.1172/JCI112491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hogg N., MacDonald S., Slusarenko M., Beverley P. C. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology. 1984 Dec;53(4):753–767. [PMC free article] [PubMed] [Google Scholar]
  14. Hogg N., Takacs L., Palmer D. G., Selvendran Y., Allen C. The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar;16(3):240–248. doi: 10.1002/eji.1830160306. [DOI] [PubMed] [Google Scholar]
  15. Hynes R. O. Integrins: a family of cell surface receptors. Cell. 1987 Feb 27;48(4):549–554. doi: 10.1016/0092-8674(87)90233-9. [DOI] [PubMed] [Google Scholar]
  16. Koenig S., Gendelman H. E., Orenstein J. M., Dal Canto M. C., Pezeshkpour G. H., Yungbluth M., Janotta F., Aksamit A., Martin M. A., Fauci A. S. Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. Science. 1986 Sep 5;233(4768):1089–1093. doi: 10.1126/science.3016903. [DOI] [PubMed] [Google Scholar]
  17. Larson R. S., Springer T. A. Structure and function of leukocyte integrins. Immunol Rev. 1990 Apr;114:181–217. doi: 10.1111/j.1600-065x.1990.tb00565.x. [DOI] [PubMed] [Google Scholar]
  18. Lifson J. D., Reyes G. R., McGrath M. S., Stein B. S., Engleman E. G. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science. 1986 May 30;232(4754):1123–1127. doi: 10.1126/science.3010463. [DOI] [PubMed] [Google Scholar]
  19. Limb G. A., Hamblin A. S., Wolstencroft R. A., Dumonde D. C. Rapid cytokine up-regulation of integrins, complement receptor 1 and HLA-DR on monocytes but not on lymphocytes. Immunology. 1992 Sep;77(1):88–94. [PMC free article] [PubMed] [Google Scholar]
  20. Limb G. A., Hamblin A. S., Wolstencroft R. A., Dumonde D. C. Selective up-regulation of human granulocyte integrins and complement receptor 1 by cytokines. Immunology. 1991 Dec;74(4):696–702. [PMC free article] [PubMed] [Google Scholar]
  21. Lähdevirta J., Maury C. P., Teppo A. M., Repo H. Elevated levels of circulating cachectin/tumor necrosis factor in patients with acquired immunodeficiency syndrome. Am J Med. 1988 Sep;85(3):289–291. doi: 10.1016/0002-9343(88)90576-1. [DOI] [PubMed] [Google Scholar]
  22. Malhotra V., Hogg N., Sim R. B. Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur J Immunol. 1986 Sep;16(9):1117–1123. doi: 10.1002/eji.1830160915. [DOI] [PubMed] [Google Scholar]
  23. McCarthy D., Taylor M. J., Bernhagen J., Perry J. D., Hamblin A. S. Leucocyte integrin and CR1 expression on peripheral blood leucocytes of patients with rheumatoid arthritis. Ann Rheum Dis. 1992 Mar;51(3):307–312. doi: 10.1136/ard.51.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Miller L. J., Bainton D. F., Borregaard N., Springer T. A. Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface. J Clin Invest. 1987 Aug;80(2):535–544. doi: 10.1172/JCI113102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. O'Shea S., Rostron T., Hamblin A. S., Palmer S. J., Banatvala J. E. Quantitation of HIV: correlation with clinical, virological, and immunological status. J Med Virol. 1991 Sep;35(1):65–69. doi: 10.1002/jmv.1890350114. [DOI] [PubMed] [Google Scholar]
  26. Petit A. J., Terpstra F. G., Miedema F. Human immunodeficiency virus infection down-regulates HLA class II expression and induces differentiation in promonocytic U937 cells. J Clin Invest. 1987 Jun;79(6):1883–1889. doi: 10.1172/JCI113032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Phillips A. N., Lee C. A., Elford J., Janossy G., Timms A., Bofill M., Kernoff P. B. Serial CD4 lymphocyte counts and development of AIDS. Lancet. 1991 Feb 16;337(8738):389–392. doi: 10.1016/0140-6736(91)91166-r. [DOI] [PubMed] [Google Scholar]
  28. Rosenberg Z. F., Fauci A. S. The immunopathogenesis of HIV infection. Adv Immunol. 1989;47:377–431. doi: 10.1016/s0065-2776(08)60665-3. [DOI] [PubMed] [Google Scholar]
  29. Rossen R. D., Smith C. W., Laughter A. H., Noonan C. A., Anderson D. C., McShan W. M., Hurvitz M. Y., Orson F. M. HIV-1-stimulated expression of CD11/CD18 integrins and ICAM-1: a possible mechanism for extravascular dissemination of HIV-1-infected cells. Trans Assoc Am Physicians. 1989;102:117–130. [PubMed] [Google Scholar]
  30. Shakoor Z., Hamblin A. S. Increased CD11/CD18 expression on peripheral blood leucocytes of patients with sarcoidosis. Clin Exp Immunol. 1992 Oct;90(1):99–105. doi: 10.1111/j.1365-2249.1992.tb05839.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Valentin A., Lundin K., Patarroyo M., Asjö B. The leukocyte adhesion glycoprotein CD18 participates in HIV-1-induced syncytia formation in monocytoid and T cells. J Immunol. 1990 Feb 1;144(3):934–937. [PubMed] [Google Scholar]
  32. Vedder N. B., Harlan J. M. Increased surface expression of CD11b/CD18 (Mac-1) is not required for stimulated neutrophil adherence to cultured endothelium. J Clin Invest. 1988 Mar;81(3):676–682. doi: 10.1172/JCI113372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Vermot Desroches C., Rigal D. Leukocyte function-associated antigen-1 expression on peripheral blood mononuclear cell subsets in HIV-1 seropositive patients. Clin Immunol Immunopathol. 1990 Aug;56(2):159–168. doi: 10.1016/0090-1229(90)90138-g. [DOI] [PubMed] [Google Scholar]
  34. Vyakarnam A., Matear P., Meager A., Kelly G., Stanley B., Weller I., Beverley P. Altered production of tumour necrosis factors alpha and beta and interferon gamma by HIV-infected individuals. Clin Exp Immunol. 1991 Apr;84(1):109–115. doi: 10.1111/j.1365-2249.1991.tb08132.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wrightham M., Schimpf A., Pennington T. H., Walker F., Sewell H. F. HIV induces modulation of functionally important cellular antigens. Clin Exp Immunol. 1991 Jul;85(1):75–79. doi: 10.1111/j.1365-2249.1991.tb05685.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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