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. 1981 Nov;44(3):577–583.

Immunological studies of the human placenta: functional and morphological analysis of pregnancy-associated plasma protein A (PAPP-A).

J A McIntyre, B Hsi, W P Faulk, A Klopper, R Thomson
PMCID: PMC1554973  PMID: 6172371

Abstract

A blood protein, pregnancy-associated plasma protein A (PAPP-A), has been isolated from the plasma of pregnant women. An antiserum to this protein has been prepared and used to localize the protein within the placenta by immunofluorescence. It was found to be inconstantly present in the syncytiotrophoblast and intervillous fibrin. Neither antiserum nor antigen exerts a cytotoxic effect on peripheral blood lymphocytes, and neither affected blastogenic responses of lymphocytes to mitogens or in mixed lymphocyte culture reactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bischof P., Hughes G., Klopper A. Relationship of obstetric parameters to the concentration of pregnancy-associated plasma protein A. Am J Obstet Gynecol. 1980 Nov 1;138(5):494–499. doi: 10.1016/0002-9378(80)90275-6. [DOI] [PubMed] [Google Scholar]
  2. Bischof P. Purification and characterization of pregnancy associated plasma protein A (PAPP-A). Arch Gynecol. 1979;227(4):315–326. doi: 10.1007/BF02109920. [DOI] [PubMed] [Google Scholar]
  3. Faulk W. P., Hsi B. L., Stevens P. J. Transferrin and transferrin receptors in carcinoma of the breast. Lancet. 1980 Aug 23;2(8191):390–392. doi: 10.1016/s0140-6736(80)90440-7. [DOI] [PubMed] [Google Scholar]
  4. Faulk W. P., Johnson P. M. Immunological studies of human placentae: identification and distribution of proteins in mature chorionic villi. Clin Exp Immunol. 1977 Feb;27(2):365–375. [PMC free article] [PubMed] [Google Scholar]
  5. Faulk W. P., Temple A., Lovins R. E., Smith N. Antigens of human trophoblasts: a working hypothesis for their role in normal and abnormal pregnancies. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1947–1951. doi: 10.1073/pnas.75.4.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gall S. A., Halbert S. P. Antigenic constituents in pregnancy plasma which are undetectable in normal non-pregnant female or male plasma. Int Arch Allergy Appl Immunol. 1972;42(4):503–515. doi: 10.1159/000230632. [DOI] [PubMed] [Google Scholar]
  7. Hughes G., Bischof P., Wilson G., Klopper A. Assay of a placental protein to determine fetal risk. Br Med J. 1980 Mar 8;280(6215):671–673. doi: 10.1136/bmj.280.6215.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lin T. M., Halbert S. P., Kiefer D., Spellacy W. N. Three pregnancy-associated human plasma proteins: purification, monospecific antisera and immunological indentification. Int Arch Allergy Appl Immunol. 1974;47(1):35–53. doi: 10.1159/000231199. [DOI] [PubMed] [Google Scholar]
  11. Lin T. M., Halbert S. P. Placental localization of human pregnancy--associated plasma proteins. Science. 1976 Sep 24;193(4259):1249–1252. doi: 10.1126/science.60782. [DOI] [PubMed] [Google Scholar]
  12. McCormick J. N., Faulk W. P., Fox H., Fudenberg H. H. Immunohistological and elution studies of the human placenta. J Exp Med. 1971 Jan 1;133(1):1–18. doi: 10.1084/jem.133.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McIntyre J. A., Turner R. E., 3rd, LeRoy E. C. Human leukocyte antigens (HLA). South Med J. 1978 Oct;71(10):1269–1279. doi: 10.1097/00007611-197810000-00021. [DOI] [PubMed] [Google Scholar]
  14. Smith R., Bischof P., Hughes G., Klopper A. Studies on pregnancy-associated plasma protein A in the third trimester of pregnancy. Br J Obstet Gynaecol. 1979 Nov;86(11):882–887. doi: 10.1111/j.1471-0528.1979.tb10716.x. [DOI] [PubMed] [Google Scholar]
  15. Sutcliffe R. G., Kukulska-Langlands B. M., Coggins J. R., Hunter J. B., Gore C. H. Studies on human pregnancy-associated plasma protein A. Purification by affinity chromatography and structural comparisons with alpha 2-macroglobulin. Biochem J. 1980 Dec 1;191(3):799–809. doi: 10.1042/bj1910799. [DOI] [PMC free article] [PubMed] [Google Scholar]

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