Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 Dec;44(4):755–763.

The role of accessory cells and T cell-growth factor in induction of cytotoxic T lymphocytes against herpes simplex virus antigens.

D S Schmid, H S Larsen, B T Rouse
PMCID: PMC1554992  PMID: 6274793

Abstract

The roles of accessory cells and T cell-growth factor (TCGF) in the in vivo induction of herpes simplex type 1 (HSV) specific cytotoxic lymphocytes (CTL) were evaluated. Spleen cells from animals infected with HSV 4-6 weeks previously were depleted of adherent cells by passage over Sephadex G10. Unlike intact cells, such depleted spleen cells failed to respond by producing H-2 restricted virus-specific CTL upon culture for 5 days with infectious HSV. The CTL response could be restored either by adding normal genetically compatible peritoneal cells as accessory cells or by the addition of TCGF. To obtain optimum restoration accessory cells needed to be added soon after culture initiation but with TCGF addition, partial restoration was evident when added as late as 72 hr after culture. TCGF also permitted intact spleen cells to respond to heat-inactivated virus. The results are interpreted to indicate that accessory cells are essentially required for the presentation of virus to specific helper cells with such cells responding by the production of TCGF. The results also indicate that certain forms of virus may trigger the response of CTL precursors but not the response of helper cells.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Degiovanni G., Cerottini J. C., Brunner K. T. Generation of cytolytic T lymphocytes in vitro. XI. Accessory cell requirement in secondary responses to particulate alloantigen. Eur J Immunol. 1980 Jan;10(1):40–45. doi: 10.1002/eji.1830100109. [DOI] [PubMed] [Google Scholar]
  2. Farr A. G., Wechter W. J., Kiely J. M., Unanue E. R. Induction of cytocidal macrophages after in vitro interactions between Listeria-immune T cells and macrophages--role of H-2. J Immunol. 1979 Jun;122(6):2405–2412. [PubMed] [Google Scholar]
  3. Fyfe D. A., Finke J. H. Soluble helper factor(s) participates in the generation of cell mediated cytotoxicity directed against syngeneic tumor cells. J Immunol. 1979 Mar;122(3):1156–1161. [PubMed] [Google Scholar]
  4. Glasebrook A. L., Fitch F. W. Alloreactive cloned T cell lines. I. Interactions between cloned amplifier and cytolytic T cell lines. J Exp Med. 1980 Apr 1;151(4):876–895. doi: 10.1084/jem.151.4.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glaser M. Macrophage requirement for in vitro generation of specific, secondary cell-mediated cytotoxicity against SV 40-induced tumor-associated antigens in mice. Eur J Immunol. 1980 May;10(5):342–346. doi: 10.1002/eji.1830100505. [DOI] [PubMed] [Google Scholar]
  6. Hapel A. J., Bablanian R., Cole G. A. Inductive requirements for the generation of virus-specific T lymphocytes. I. The nature of the host cell-virus interaction that triggers secondary poxvirus-specific cytotoxic T lymphocyte induction. J Immunol. 1978 Aug;121(2):736–743. [PubMed] [Google Scholar]
  7. Koszinowski U. H., Gething M. J. Generation of virus-specific cytotoxic T cells in vitro. II. Induction requirements with functionally inactivated virus preparations. Eur J Immunol. 1980 Jan;10(1):30–35. doi: 10.1002/eji.1830100107. [DOI] [PubMed] [Google Scholar]
  8. Lause D. B. Suppressed lymphocyte reactivity following lymphocyte interaction with macrophages. J Reticuloendothel Soc. 1979 Dec;26(6):775–786. [PubMed] [Google Scholar]
  9. Lawman M. J., Rouse B. T., Courtney R. J., Walker R. D. Cell-mediated immunity against herpes simplex induction of cytotoxic T lymphocytes. Infect Immun. 1980 Jan;27(1):133–139. doi: 10.1128/iai.27.1.133-139.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Okada M., Klimpel G. R., Kuppers R. C., Henney C. S. The differentiation of cytotoxic T cells in vitro. I. Amplifying factor(s) in the primary response is Lyt 1 + cell dependent. J Immunol. 1979 Jun;122(6):2527–2533. [PubMed] [Google Scholar]
  11. Pang T., McKenzie I. F., Blanden R. V. Cooperation between mouse T-cell subpopulations in the cell-mediated response to a natural poxvirus pathogen. Cell Immunol. 1976 Oct;26(2):153–159. doi: 10.1016/0008-8749(76)90359-2. [DOI] [PubMed] [Google Scholar]
  12. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rouse B. T., Lawman M. J. Induction of cytotoxic T lymphocytes against herpes simplex virus type i: role of accessory cells and amplifying factor. J Immunol. 1980 May;124(5):2341–2346. [PubMed] [Google Scholar]
  14. Schwartz R. H., Yano A., Paul W. E. Interaction between antigen-presenting cells and primed T lymphocytes: an assessment of Ir gene expression in the antigen-presenting cell. Immunol Rev. 1978;40:153–180. doi: 10.1111/j.1600-065x.1978.tb00405.x. [DOI] [PubMed] [Google Scholar]
  15. Shaw J., Caplan B., Paetkau V., Pilarski L. M., Delovitch T. L., McKenzie I. F. Cellular origins of co-stimulator (IL2) and its activity in cytotoxic T lymphocyte responses. J Immunol. 1980 May;124(5):2231–2239. [PubMed] [Google Scholar]
  16. Smith K. A., Baker P. E., Gillis S., Ruscetti F. W. Functional and molecular characteristics of T-cell growth factor. Mol Immunol. 1980 May;17(5):579–589. doi: 10.1016/0161-5890(80)90156-x. [DOI] [PubMed] [Google Scholar]
  17. Smith K. A. T-cell growth factor. Immunol Rev. 1980;51:337–357. doi: 10.1111/j.1600-065x.1980.tb00327.x. [DOI] [PubMed] [Google Scholar]
  18. Swain S. L., Dutton R. W. Production of Con A-induced helper T cell replacing factor requires a T cell and an Ia-positive non-T cells. J Immunol. 1980 Jan;124(1):437–444. [PubMed] [Google Scholar]
  19. Swierkosz J. E., Rock K., Marrack P., Kappler J. W. The role of H-2 linked genes in helper T-cell function. II. Isolation on antigen-pulsed macrophages of two separate populations of F1 helper T cells each specific for antigen and one set of parental H-2 products. J Exp Med. 1978 Feb 1;147(2):554–570. doi: 10.1084/jem.147.2.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ting C. C., Rodrigues D. Immunoregulatory circuit among macrophage subsets for T cell-mediated cytotoxic response to tumor cells. J Immunol. 1980 Mar;124(3):1039–1044. [PubMed] [Google Scholar]
  21. Wagner H., Pfizenmaier K., Röllinghoff M. The role of the major histocompatibility gene complex in murine cytotoxic T cell responses. Adv Cancer Res. 1980;31:77–124. doi: 10.1016/s0065-230x(08)60657-0. [DOI] [PubMed] [Google Scholar]
  22. Wagner H., Röllinghoff M. T-T-cell interactions during the vitro cytotoxic allograft responses. I. Soluble products from activated Lyl+ T cells trigger autonomously antigen-primed Ly23+ T cells to cell proliferation and cytolytic activity. J Exp Med. 1978 Dec 1;148(6):1523–1538. doi: 10.1084/jem.148.6.1523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Woodward J. G., Fernandez P. A., Daynes R. A. Cell-mediated immune response to syngeneic UV-induced tumors. III. Requirement for an Ia+ macrophage in the in vitro differentiation of cytotoxic T lymphocytes. J Immunol. 1979 Apr;122(4):1196–1202. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES