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. 1981 Dec;44(4):773–780.

Soluble immune complexes binding to human monocytes and polymorphonuclear leucocytes.

F G Cosio, S K Ackerman, S D Douglas, P S Friend, A F Michael
PMCID: PMC1555001  PMID: 7319559

Abstract

Soluble human serum albumin anti-albumin immune complexes (IC) have been shown to bind to freshly isolated human peripheral blood monocytes and polymorphonuclear leucocytes (PMN) in vitro. Binding sites on both cell types were saturable and specifically inhibited by heat aggregated IgG1 and IgG3 subclasses. PMN contained a greater number of binding sites than monocytes although the affinity was similar for both cell types. The enhanced binding of IC by both cell types observed after incubation at low pH (pH 6.0) was a consequence of increased affinity of the PMN binding site and an increase in the number of sites in monocytes. Binding of IC by both cell types was inhibited by normal human serum. Enhanced IC binding associated with enhanced affinity and number of sites was observed in PMN and monocytes preincubated in suspension with trypsin. However, monocytes exposed to trypsin while adherent to microexudate coated flasks demonstrated a marked increase in affinity without any change in the number of sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackerman S. K., Douglas S. D. Purification of human monocytes on microexudate-coated surfaces. J Immunol. 1978 Apr;120(4):1372–1374. [PubMed] [Google Scholar]
  2. Alexander E. L., Titus J. A., Segal D. M. Human leukocyte Fc (IgG) receptors: quantitation and affinity with radiolabeled affinity cross-linked rabbit IgG. J Immunol. 1979 Jul;123(1):295–302. [PubMed] [Google Scholar]
  3. Arend W. P., Mannik M. In vitro adherence of soluble immune complexes to macrophages. J Exp Med. 1972 Sep 1;136(3):514–531. doi: 10.1084/jem.136.3.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bevilacqua M. P., Amrani D., Mosesson M. W., Bianco C. Receptors for cold-insoluble globulin (plasma fibronectin) on human monocytes. J Exp Med. 1981 Jan 1;153(1):42–60. doi: 10.1084/jem.153.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Finbloom D. S., Plotz P. H. Studies of reticuloendothelial function in the mouse with model immune complexes. I. Serum clearance and tissue uptake in normal C3H mice. J Immunol. 1979 Oct;123(4):1594–1599. [PubMed] [Google Scholar]
  6. Frank M. M., Hamburger M. I., Lawley T. J., Kimberly R. P., Plotz P. H. Defective reticuloendothelial system Fc-receptor function in systemic lupus erythematosus. N Engl J Med. 1979 Mar 8;300(10):518–523. doi: 10.1056/NEJM197903083001002. [DOI] [PubMed] [Google Scholar]
  7. Haakenstad A. O., Mannik M. Saturation of the reticuloendothelial system with soluble immune complexes. J Immunol. 1974 May;112(5):1939–1948. [PubMed] [Google Scholar]
  8. Hamburger M. I., Moutsopoulos H. M., Lawley T. J., Frank M. M. Sjögren's syndrome: a defect in reticuloendothelial system Fc-receptor-specific clearance. Ann Intern Med. 1979 Oct;91(4):534–538. doi: 10.7326/0003-4819-91-4-534. [DOI] [PubMed] [Google Scholar]
  9. Jaffe C. J., Vierling J. M., Jones E. A., Lawley T. J., Frank M. M. Receptor specific clearance by the reticuloendothelial system in chronic liver diseases. Demonstration of defective C3b-specific clearance in primary biliary cirrhosis. J Clin Invest. 1978 Nov;62(5):1069–1077. doi: 10.1172/JCI109212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurlander R. J. Reversible and irreversible loss of Fc receptor function of human monocytes as a consequence of interaction with immunoglobulin G. J Clin Invest. 1980 Oct;66(4):773–781. doi: 10.1172/JCI109915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leslie R. G. The binding of soluble immune complexes of guinea pig IgG2 to homologous peritoneal macrophages. Determination of the avidity constants at 4 degrees C. Eur J Immunol. 1980 May;10(5):317–322. doi: 10.1002/eji.1830100502. [DOI] [PubMed] [Google Scholar]
  13. Lockwood C. M., Worlledge S., Nicholas A., Cotton C., Peters D. K. Reversal of impaired splenic function in patients with nephritis or vasculitis (or both) by plasma exchange. N Engl J Med. 1979 Mar 8;300(10):524–530. doi: 10.1056/NEJM197903083001003. [DOI] [PubMed] [Google Scholar]
  14. Marucci A. A., DiStefano H. S., Dougherty R. M. Preparation and use of soluble ferritin-antiferritin complexes as a specific marker for immunoelectron microscopy. J Histochem Cytochem. 1974 Jan;22(1):35–39. doi: 10.1177/22.1.35. [DOI] [PubMed] [Google Scholar]
  15. Segal D. M., Hurwitz E. Binding of affinity cross-linked oligomers of IgG to cells bearing Fc receptors. J Immunol. 1977 Apr;118(4):1338–1337. [PubMed] [Google Scholar]
  16. Williams B. D., Pussell B. A., Lockwood C. M., Cotton C. Defective reticuloendothelial system function in rheumatoid arthritis. Lancet. 1979 Jun 23;1(8130):1311–1314. doi: 10.1016/s0140-6736(79)91947-0. [DOI] [PubMed] [Google Scholar]
  17. Yagawa K., Onoue K., Aida Y. Structural studies of Fc receptors. I. Binding properties, solubilization, and partial characterization of fc receptors of macrophages. J Immunol. 1979 Jan;122(1):366–373. [PubMed] [Google Scholar]
  18. Zuckerman S. H., Douglas S. D. Dynamics of the macrophage plasma membrane. Annu Rev Microbiol. 1979;33:267–307. doi: 10.1146/annurev.mi.33.100179.001411. [DOI] [PubMed] [Google Scholar]

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