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. 1981 Jul;43(3):483–491.

The growth-promoting effect of bacterial iron for serum-exposed bacteria.

M W Mellencamp, M A McCabe, I Kochan
PMCID: PMC1555044  PMID: 6454656

Abstract

Bacterial ability to obtain iron in bovine serum or in media containing transferrin (Tr) or conalbumin (Ca) was investigated by using serum-resistant (virulent) and serum-sensitive (avirulent) strains of Escherichia coli and Salmonella typhimurium. Bacteria growing in bovine serum enriched with radioactive iron-saturated Tr or with radioactive iron-saturated enterobactin (E) did not acquire radioactive iron. It has been found that the passage of siderophore (Si)-iron complexes into bacteria is blocked in serum by Tr and in Ca-containing medium by Ca. The investigation of bacterial ability to take iron in synthetic media showed that bacteria take in Si-bound but no Tr-bound radioactive iron. In the absence of free iron, the growth of serum-exposed virulent bacteria was supported by their stored iron. Virulent bacteria passaged in medium void of usable iron became depleted in stored iron and did not grow in animal sera unless sera were enriched by the addition of exogenous iron. Experiments with serum-exposed avirulent bacteria showed that their growth in Si-enriched serum should not be attributed to the iron-providing activity of Si but to the stimulating effect of Si which facilitates the use of stored iron. As distinct from avirulent bacteria, virulent bacteria used stored iron without the stimulating activity of extracellular Si.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arceneaux J. E., Byers B. R. Ferric hydroxamate transport without subsequent iron utilization in Bacillus megaterium. J Bacteriol. 1976 Sep;127(3):1324–1330. doi: 10.1128/jb.127.3.1324-1330.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bullen J. J., Rogers H. J., Griffiths E. Role of iron in bacterial infection. Curr Top Microbiol Immunol. 1978;80:1–35. doi: 10.1007/978-3-642-66956-9_1. [DOI] [PubMed] [Google Scholar]
  3. Fitzgerald S. P., Rogers H. J. Bacteriostatic effect of serum: role of antibody to lipopolysaccharide. Infect Immun. 1980 Feb;27(2):302–308. doi: 10.1128/iai.27.2.302-308.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forsberg C. M., Bullen J. J. The effect of passage and iron on the virulence of Pseudomonas aeruginosa. J Clin Pathol. 1972 Jan;25(1):65–68. doi: 10.1136/jcp.25.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Golden C. A., Kochan I., Spriggs D. R. Role of mycobactin in the growth and virulence of tubercle bacilli. Infect Immun. 1974 Jan;9(1):34–40. doi: 10.1128/iai.9.1.34-40.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kochan I., Cahall D. L., Golden C. A. Employment of tuberculostasis in serum-agar medium for the study of production and activity of Mycobactin. Infect Immun. 1971 Aug;4(2):130–137. doi: 10.1128/iai.4.2.130-137.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kochan I., Kvach J. T., Wiles T. I. Virulence-associated acquisition of iron in mammalian serum by Escherichia coli. J Infect Dis. 1977 Apr;135(4):623–632. doi: 10.1093/infdis/135.4.623. [DOI] [PubMed] [Google Scholar]
  8. Kochan I. The role of iron in bacterial infections, with special consideration of host-tubercle bacillus interaction. Curr Top Microbiol Immunol. 1973;60:1–30. doi: 10.1007/978-3-642-65502-9_1. [DOI] [PubMed] [Google Scholar]
  9. Kochan I., Wasynczuk J., McCabe M. A. Effects of injected iron and siderophores on infections in normal and immune mice. Infect Immun. 1978 Nov;22(2):560–567. doi: 10.1128/iai.22.2.560-567.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kvach J. T., Wiles T. I., Mellencamp M. W., Kochan I. Use of transferrin-iron enterobactin complexes as the source of iron by serum-exposed bacteria. Infect Immun. 1977 Nov;18(2):439–445. doi: 10.1128/iai.18.2.439-445.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leffell M. S., Spitznagel J. K. Fate of human lactoferrin and myeloperoxidase in phagocytizing human neutrophils: effects of immunoglobulin G subclasses and immune complexes coated on latex beads. Infect Immun. 1975 Oct;12(4):813–820. doi: 10.1128/iai.12.4.813-820.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Liu P. V., Shokrani F. Biological activities of pyochelins: iron-chelating agents of Pseudomonas aeruginosa. Infect Immun. 1978 Dec;22(3):878–890. doi: 10.1128/iai.22.3.878-890.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miles A. A., Khimji P. L. Enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity. J Med Microbiol. 1975 Nov;8(4):477–490. doi: 10.1099/00222615-8-4-477. [DOI] [PubMed] [Google Scholar]
  14. Moore D. G., Yancey R. J., Lankford C. E., Earhart C. F. Bacteriostatic enterochelin-specific immunoglobulin from normal human serum. Infect Immun. 1980 Feb;27(2):418–423. doi: 10.1128/iai.27.2.418-423.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Payne S. M., Finkelstein R. A. Pathogenesis and immunology of experimental gonococcal infection: role of iron in virulence. Infect Immun. 1975 Dec;12(6):1313–1318. doi: 10.1128/iai.12.6.1313-1318.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Payne S. M., Finkelstein R. A. The critical role of iron in host-bacterial interactions. J Clin Invest. 1978 Jun;61(6):1428–1440. doi: 10.1172/JCI109062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rogers H. J. Iron-Binding Catechols and Virulence in Escherichia coli. Infect Immun. 1973 Mar;7(3):445–456. doi: 10.1128/iai.7.3.445-456.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Snow G. A. Mycobactins: iron-chelating growth factors from mycobacteria. Bacteriol Rev. 1970 Jun;34(2):99–125. doi: 10.1128/br.34.2.99-125.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weinberg E. D. Nutritional immunity. Host's attempt to withold iron from microbial invaders. JAMA. 1975 Jan 6;231(1):39–41. doi: 10.1001/jama.231.1.39. [DOI] [PubMed] [Google Scholar]
  21. Williams P. H. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. doi: 10.1128/iai.26.3.925-932.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yancey R. J., Breeding S. A., Lankford C. E. Enterochelin (enterobactin): virulence factor for Salmonella typhimurium. Infect Immun. 1979 Apr;24(1):174–180. doi: 10.1128/iai.24.1.174-180.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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