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. 1981 Jul;43(3):563–572.

Characterization of lymphocyte subpopulations which exhibit enhanced DNA synthesis in vitro in DNFB contact sensitive guinea-pigs.

R J Scheper, L Polak
PMCID: PMC1555050  PMID: 6972912

Abstract

Different lymphocyte subpopulations were prepared from lymph nodes and spleen obtained from 2.4-dinitrofluorobenzene/Freund's complete adjuvant (DNFB/FCA) sensitized guinea-pigs. The capacity to react in vitro to secondary antigenic (dinitrophenyl-modified syngeneic macrophages, DNP-MO) stimulation by an increased DNA synthesis, appeared to be restricted to those T cells which lack receptors for IgG-Fc (T gamma -). We confirmed the existence in this species of an IgG-Fc receptor-positive T-cell subset, which is particularly represented in the spleen (up to 56%). These T gamma + cells apparently are not of monocyte lineage as they show strong PHA-responsiveness and a lack of non-specific esterase staining, whereas the majority of these cells form RRBC-rosettes. Neither B nor T gamma + cells appeared to act as suppressor cells in the secondary DNP-MO-driven proliferative response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantor H., Boyse E. A. Regulation of cellular and humoral immune responses by T-cell subclasses. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):23–32. doi: 10.1101/sqb.1977.041.01.006. [DOI] [PubMed] [Google Scholar]
  2. Claman H. N., Miller S. D., Sy M. S., Moorhead J. W. Suppressive mechanisms involving sensitization and tolerance in contact allergy. Immunol Rev. 1980;50:105–132. doi: 10.1111/j.1600-065x.1980.tb00309.x. [DOI] [PubMed] [Google Scholar]
  3. Gupta S., Good R. A. Human T cell subpopulations as defined by Fc receptors. Thymus. 1979 Nov;1(3):135–149. [PubMed] [Google Scholar]
  4. Heijnen C. J., UytdeHaag F., Gmelig-Meyling F. H., Ballieux R. E. Localization of human antigen-specific helper and suppressor function in distinct T-cell subpopulations. Cell Immunol. 1979 Mar 15;43(2):282–292. doi: 10.1016/0008-8749(79)90173-4. [DOI] [PubMed] [Google Scholar]
  5. Katz S. I., Parker D., Turk J. L. B-cell suppression of delayed hypersensitivity reactions. Nature. 1974 Oct 11;251(5475):550–551. doi: 10.1038/251550a0. [DOI] [PubMed] [Google Scholar]
  6. Kaufmann S. H., Simon M. M., Hahn H. Specific Lyt 123 cells are involved in protection against Listeria monocytogenes and in delayed-type hypersensitivity to listerial antigens. J Exp Med. 1979 Oct 1;150(4):1033–1038. doi: 10.1084/jem.150.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuribayashi K., Takabayashi A., Miyama M., Yamada J., Masuda T. Immunological properties of Fc receptor on lymphocytes. 7. Suppression of mixed lymphocyte reaction by Fc receptor-bearing splenic lymphocytes. Cell Immunol. 1979 Sep 1;46(2):373–383. doi: 10.1016/0008-8749(79)90424-6. [DOI] [PubMed] [Google Scholar]
  8. Larsson E. L., Andersson J., Coutinho A. Functional consequences of sheep red blood cell rosetting for human T cells: gain of reactivity to mitogenic factors. Eur J Immunol. 1978 Oct;8(10):693–696. doi: 10.1002/eji.1830081005. [DOI] [PubMed] [Google Scholar]
  9. Lipsky P. E., Rosenthal A. S. The induction and regulation of guinea pig B-lymphocyte proliferation in vitro. J Immunol. 1976 Nov;117(5 Pt 1):1594–1602. [PubMed] [Google Scholar]
  10. Loos J. A., Roos D. Ficoll-isopaque gradients for the determination of density distributions of human blood lymphocytes and other reticulo-endothelial cells. Exp Cell Res. 1974 Jun;86(2):333–341. doi: 10.1016/0014-4827(74)90721-6. [DOI] [PubMed] [Google Scholar]
  11. Moretta L., Ferrarini M., Mingari M. C., Moretta A., Webb S. R. Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol. 1976 Dec;117(6):2171–2174. [PubMed] [Google Scholar]
  12. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mullink H., Von Blomberg M., Wilders M. M., Drexhage H. A., Alons C. L. A simple cytochemical method for distinguishing EAC rosettes formed by lymphocytes and monocytes. J Immunol Methods. 1979;29(2):133–137. doi: 10.1016/0022-1759(79)90062-0. [DOI] [PubMed] [Google Scholar]
  14. Parker D., Turk J. L., Scheper R. J. Central and peripheral action of suppressor cells in contact sensitivity in the guinea-pig. Immunology. 1976 Apr;30(4):593–597. [PMC free article] [PubMed] [Google Scholar]
  15. Phanuphak P., Moorhead J. W., Claman H. N. Tolerance and contact sensitivity to DNFB in mice. I. In vivo detection by ear swelling and correlation with in vitro cell stimulation. J Immunol. 1974 Jan;112(1):115–123. [PubMed] [Google Scholar]
  16. Reinherz E. L., Moretta L., Roper M., Breard J. M., Mingari M. C., Cooper M. D., Schlossman S. F. Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med. 1980 Apr 1;151(4):969–974. doi: 10.1084/jem.151.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reinherz E. L., Schlossman S. F. Current concepts in immunology: Regulation of the immune response--inducer and suppressor T-lymphocyte subsets in human beings. N Engl J Med. 1980 Aug 14;303(7):370–373. doi: 10.1056/NEJM198008143030704. [DOI] [PubMed] [Google Scholar]
  18. Samarut C., Revillard J. P. Modulation of lymphocyte receptors for IgG inhibits responding cells in the mixed lymphocyte reaction. Transplantation. 1979 Apr;27(4):283–285. doi: 10.1097/00007890-197904000-00016. [DOI] [PubMed] [Google Scholar]
  19. Scheper R. J., van Maarsseveen T. C., van Dinther-Janssen A. C. Comparative migration of guinea pig T and B lymphocytes from capillary tubes. Cell Immunol. 1980 Jul 15;53(1):19–28. doi: 10.1016/0008-8749(80)90422-0. [DOI] [PubMed] [Google Scholar]
  20. Stingl G., Pichler W. J., Shevach E. M. T-Zell-subpopulationen im Meerschweinchen. Wien Klin Wochenschr. 1978 Oct 27;90(20):741–744. [PubMed] [Google Scholar]
  21. Stout R. D., Waksal S. D., Herzenberg L. A. The Fc receptor on thymus-derived lymphocytes. III. Mixed lymphocyte reactivity and cell-mediated lympholytic activity of Fc- and Fc+ T lymphocytes. J Exp Med. 1976 Jul 1;144(1):54–68. doi: 10.1084/jem.144.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Turk J. L., Polak L., Parker D. Control mechanisms in delayed-type hypersensitivity. Br Med Bull. 1976 May;32(2):165–170. doi: 10.1093/oxfordjournals.bmb.a071350. [DOI] [PubMed] [Google Scholar]
  23. Wilson A. B., Gurner B. W. Increased affinity of guinea pig thymocytes and thymus-dependent lymphocytes for papain-treated rabbit erythrocytes compared to untreated erythrocytes. J Immunol Methods. 1975 Jun;7(2-3):163–168. doi: 10.1016/0022-1759(75)90013-7. [DOI] [PubMed] [Google Scholar]
  24. Wilson A. B., Prichard-Thomas S., Coombs R. R. Receptors for activated C3 on thymus-dependent (T) lymphocytes of normal guinea-pigs. Immunology. 1979 Jun;37(2):377–384. [PMC free article] [PubMed] [Google Scholar]
  25. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  26. Zembala M., Asherson G. L., Noworolski J., Mayhew B. Contact sensitivity to picryl chloride: the occurrence of B suppressor cells in the lymph nodes and spleen of immunized mice. Cell Immunol. 1976 Aug;25(2):266–278. doi: 10.1016/0008-8749(76)90117-9. [DOI] [PubMed] [Google Scholar]
  27. Zembala M., Asherson G. L. The role of T cells in the passive transfer of contact sensitivity and their occurrence in the bone marrow. Eur J Immunol. 1973 Nov;3(11):667–680. doi: 10.1002/eji.1830031105. [DOI] [PubMed] [Google Scholar]
  28. Zeylemaker W. P., Roos M. T., Meyer C. J., Schellekens P. T., Eijsvoogel V. P. Separation of human lymphocyte subpopulations. I. Transformation characteristics of rosette-forming cells. Cell Immunol. 1974 Dec;14(3):346–358. doi: 10.1016/0008-8749(74)90184-1. [DOI] [PubMed] [Google Scholar]
  29. von Blomberg M., Scheper R. J. Hapten-cell conjugates in DNCB contact sensitivity. In vitro stimulation with DNP-conjugates optimally inducing contact sensitivity in vivo. Immunology. 1980 Feb;39(2):291–299. [PMC free article] [PubMed] [Google Scholar]

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