Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 May;43(1):101–110.

Comparative studies on antibodies to poly(ADP-ribose) in rabbits and patients with systemic lupus erythematosus.

Y Kanai, T Sugimura
PMCID: PMC1555186  PMID: 7251045

Abstract

Immunochemical studies were made on the antibodies induced in rabbits against poly(ADP-ribose) and naturally-occurring antibodies in patients with systemic lupus erythematosus. Antibodies against poly(ADP-ribose) could also be induced in rabbits by oligo(ADP-ribose) associated with rat liver histones and by a complex of poly(ADP-ribose) with methylated bovine serum albumin (MBSA). The two types of antibody were inhibited to the same extent by poly(ADP-ribose). However, the antibody induced by oligo(ADP-ribose) associated with histones was inhibited by oligo(ADP-ribose) with an average chain length if 4 ADP-ribosyl units and by phosphoribosyl adenosine monophosphate (PR-AMP) but not by mono ADP-ribose, whereas that induced by poly(ADP-ribose) was practically not inhibited by these related compounds even in excess amounts. The sera of ten cases of systemic lupus erythematosus showing high antibody activity against poly(ADP-ribose) were also examined immunochemically. It was found that the antibodies of three patients showed a similar inhibitory pattern to that of antibody induced in rabbits by oligo(ADP-ribose) associated with histones, those of three patients showed a similar pattern to that of antibody produced in rabbits by poly(ADP-ribose), and the remainder did not show either pattern. These findings suggest that oligo(ADP-ribose) associated with histones may serve as antigen to elicit naturally-occuring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus.

Full text

PDF
101

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burzio L. O., Riquelme P. T., Koide S. S. ADP ribosylation of rat liver nucleosomal core histones. J Biol Chem. 1979 Apr 25;254(8):3029–3037. [PubMed] [Google Scholar]
  2. Ferro A. M., Minaga T., Piper W. N., Kun E. Analysis of larger than tetrameric poly(adenosine diphosphoribose) by a radioimmunoassay in nuclei separated in organic solvents. Biochim Biophys Acta. 1978 Jul 24;519(2):291–305. doi: 10.1016/0005-2787(78)90083-7. [DOI] [PubMed] [Google Scholar]
  3. Hayaishi O., Ueda K. Poly(ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem. 1977;46:95–116. doi: 10.1146/annurev.bi.46.070177.000523. [DOI] [PubMed] [Google Scholar]
  4. Hilz H., Stone P. Poly(ADP-ribose) and ADP-ribosylation of proteins. Rev Physiol Biochem Pharmacol. 1976;76:1-58, 177. doi: 10.1007/BFb0027686. [DOI] [PubMed] [Google Scholar]
  5. Hughes G. R., Cohen S. A., Christian C. L. Anti-DNA activity in systemic lupus erythematosus. A diagnostic and therapeutic guide. Ann Rheum Dis. 1971 May;30(3):259–264. doi: 10.1136/ard.30.3.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kanai Y., Kawaminami Y., Miwa M., Matsushima T., Sugimura T. Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus. Nature. 1977 Jan 13;265(5590):175–177. doi: 10.1038/265175a0. [DOI] [PubMed] [Google Scholar]
  7. Kanai Y., Miwa M., Matsushima T., Sugimura T. Comparative studies on antibody and antibody production to poly(ADP-ribose) in mice. Immunology. 1978 Mar;34(3):501–508. [PMC free article] [PubMed] [Google Scholar]
  8. Kanai Y., Miwa M., Matsushima T., Sugimura T. Studies on anti-poly(adenosine diphosphate ribose) antibody. Biochem Biophys Res Commun. 1974 Jul 10;59(1):300–306. doi: 10.1016/s0006-291x(74)80206-8. [DOI] [PubMed] [Google Scholar]
  9. Kanai Y., Sugimura T., Matsushima T. Induction of specific antibodies to poly(ADP-ribose) in rabbits by double-stranded RNA, poly(A)-poly(U). Nature. 1978 Aug 24;274(5673):809–812. doi: 10.1038/274809a0. [DOI] [PubMed] [Google Scholar]
  10. Kidwell W. R., Mage M. G. Changes in poly(adenosine diphosphate-ribose) and poly(adenosine diphosphate-ribose) polymerase in synchronous HeLa cells. Biochemistry. 1976 Mar 23;15(6):1213–1217. doi: 10.1021/bi00651a006. [DOI] [PubMed] [Google Scholar]
  11. Koffler D., Carr R., Agnello V., Thoburn R., Kunkel H. G. Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease. J Exp Med. 1971 Jul 1;134(1):294–312. doi: 10.1084/jem.134.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Merchant B., Inman J. K. Hapten-specific hemolytic plaque assays usually fail to detect most of the diversity in the anti-hapten response. J Exp Med. 1977 Feb 1;145(2):372–389. doi: 10.1084/jem.145.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Minaga T., Romaschin A. D., Kirsten E., Kun E. The in vivo distribution of immunoreactive larger than tetrameric polyadenosine diphosphoribose in histone and non-histone protein fractions of rat liver. J Biol Chem. 1979 Oct 10;254(19):9663–9668. [PubMed] [Google Scholar]
  14. Mäkelä O. Single lymph node cells producing heteroclitic bacteriophage antibody. J Immunol. 1965 Aug;95(2):378–386. [PubMed] [Google Scholar]
  15. Okayama H., Ueda K., Hayaishi O. Purification of ADP-ribosylated nuclear proteins by covalent chromatography on dihydroxyboryl polyacrylamide beads and their characterization. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1111–1115. doi: 10.1073/pnas.75.3.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Okolie E. E., Shall S. The significance of antibodies to poly(adenosine diphosphate-ribose) in systemic lupus erythematosus. Clin Exp Immunol. 1979 Apr;36(1):151–164. [PMC free article] [PubMed] [Google Scholar]
  17. Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
  18. Papalian M., Lafer E., Wong R., Stollar B. D. Reaction of systemic lupus erythematosus antinative DNA antibodies with native DNA fragments from 20 to 1,200 base pairs. J Clin Invest. 1980 Feb;65(2):469–477. doi: 10.1172/JCI109690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Riquelme P. T., Burzio L. O., Koide S. S. ADP ribosylation of rat liver lysine-rich histone in vitro. J Biol Chem. 1979 Apr 25;254(8):3018–3028. [PubMed] [Google Scholar]
  20. Sakura H., Miwa M., Kanai Y., Matsushima T., Sugimura T. Formation and characterization of antibody against 2'-(5"-phosphoribosyl)-5' AMP, the monomer form of poly(adenosine diphosphate ribose). Nucleic Acids Res. 1978 Nov;5(11):4025–4038. doi: 10.1093/nar/5.11.4025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sakura H., Miwa M., Tanaka M., Kanai Y., Shimada T., Matsushima T., Sugimura T. Natural occurence of a biopolymer, poly (adenosine diphosphate ribose). Nucleic Acids Res. 1977 Aug;4(8):2903–2915. doi: 10.1093/nar/4.8.2903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schur P. H., Sandson J. Immunologic factors and clinical activity in systemic lupus erythematosus. N Engl J Med. 1968 Mar 7;278(10):533–538. doi: 10.1056/NEJM196803072781004. [DOI] [PubMed] [Google Scholar]
  23. Sugimura T., Yoshimura N., Miwa M., Nagai H., Nagao M. Studies on poly(adenosine diphosphate-ribose). XI. Purification of poly(adenosine diphosphate-ribose) on a hydroxylapatite column. Arch Biochem Biophys. 1971 Dec;147(2):660–665. doi: 10.1016/0003-9861(71)90425-5. [DOI] [PubMed] [Google Scholar]
  24. Tan E. M., Schur P. H., Carr R. I., Kunkel H. G. Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus. J Clin Invest. 1966 Nov;45(11):1732–1740. doi: 10.1172/JCI105479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tanaka M., Miwa M., Hayashi K., Kubota K., Matsushima T. Separation of oligo(adenosine diphosphate ribose) fractions with various chain lengths and terminal structures. Biochemistry. 1977 Apr 5;16(7):1485–1489. doi: 10.1021/bi00626a037. [DOI] [PubMed] [Google Scholar]
  26. Walters C. S., Wigzell H. Heteroclitic cells in anti-hapten systems: some studies at the cellular and serum level. Cell Immunol. 1972 Dec;5(4):570–578. doi: 10.1016/0008-8749(72)90107-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES